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Eccentric localization of catalase to protect chromosomes from oxidative damages during meiotic maturation in mouse oocytes

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Abstract

The maintenance of genomic integrity and stability is essential for the survival of every organism. Unfortunately, DNA is vulnerable to attack by a variety of damaging agents. Oxidative stress is a major cause of DNA damage because reactive oxygen species (ROS) are produced as by-products of normal cellular metabolism. Cells have developed eloquent antioxidant defense systems to protect themselves from oxidative damage along with aerobic metabolism. Here, we show that catalase (CAT) is present in mouse oocytes to protect the genome from oxidative damage during meiotic maturation. CAT was expressed in the nucleus to form unique vesicular structures. However, after nuclear envelope breakdown, CAT was redistributed in the cytoplasm with particular focus at the chromosomes. Inhibition of CAT activity increased endogenous ROS levels, but did not perturb meiotic maturation. In addition, CAT inhibition produced chromosomal defects, including chromosome misalignment and DNA damage. Therefore, our data suggest that CAT is required not only to scavenge ROS, but also to protect DNA from oxidative damage during meiotic maturation in mouse oocytes.

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Abbreviations

ROS:

Reactive oxygen species

DDR:

DNA damage response

CAT:

Catalase

SOD:

Superoxide dismutase

GPX:

Glutathione peroxidase

3-AT:

3-amino-1,2,4-triazole

PMSG:

Pregnant mare’s serum gonadotropin

IBMX:

3-Isobutyl-1-methylxanthine

DHR-123:

Dihydrorhodamine 123

DSB:

DNA double-strand break

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Acknowledgments

This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HI12C0737), and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013R1A1A1004766).

Author’s contributions

JO conceived and designed the experiments; Y.P, S.Y and S.C performed the experiments; Y.P and J.O analyzed the data; H. J, S.L, D.C and J.K contributed reagents, materials, analysis and tools; and J.O wrote the manuscript.

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Authors and Affiliations

  1. Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, Gyeonggi-do, 440-746, Korea

    Yong Seok Park, Seung Yeop You, Sungrae Cho, Hyuk-Joon Jeon, Sukchan Lee & Jeong Su Oh

  2. Department of East–West Medical Science, Graduate School of East–West Medical Science, Kyung Hee University, Yongin, South Korea

    Dong-Hyung Cho

  3. Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea

    Jae-Sung Kim

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  1. Yong Seok Park
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Correspondence to Jeong Su Oh.

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Park, Y.S., You, S.Y., Cho, S. et al. Eccentric localization of catalase to protect chromosomes from oxidative damages during meiotic maturation in mouse oocytes. Histochem Cell Biol 146, 281–288 (2016). https://doi.org/10.1007/s00418-016-1446-3

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