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Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars

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Abstract

This study was designed to examine developing acellular cementum in rat molars by immunohistochemistry, to elucidate (1) how Hertwig’s epithelial root sheath disintegrates and (2) whether epithelial sheath cells transform into cementoblasts through epithelial–mesenchymal transition (EMT). Initial acellular cementogenesis was divided into three developmental stages, which can be seen in three different portions of the root: portion 1, where the epithelial sheath is intact; portion 2, where the epithelial sheath becomes fragmented; and portion 3, where acellular cementogenesis begins. Antibodies against three kinds of matrix proteinases, which degrade epithelial sheath-maintaining factors, including basement membrane and desmosomes, were used to investigate proteolytic activity of the epithelial sheath. Tissue non-specific alkaline phosphatase (TNALP) and keratin were used to investigate EMT. Epithelial sheath cells showed immunoreactivity for all three enzymes at fragmentation, which suggests that epithelial sheath disintegration is enzymatically mediated. Dental follicle cells and cementoblasts showed intense immunoreactivity for TNALP, and from portion 1 through to 3, the reaction extended from the alveolar bone-related zone to the root-related zone. Cells possessing keratin/TNALP double immunoreactivity were virtually absent. Keratin-positive epithelial sheath cells showed negligible immunoreactivity for TNALP, and epithelial cells did not appear to migrate to the dental follicle. Together, these findings suggest that a transition phenotype between epithelial cells and cementoblasts does not exist in the developing dental follicle and hence that epithelial sheath cells do not undergo EMT during initial acellular cementogenesis. In brief, this study supports the notion that cementoblasts derive from the dental follicle.

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Acknowledgments

This study was supported by a grant from the Japanese Society for the Promotion of Science to T. Yamamoto (No. 22592028).

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Authors and Affiliations

  1. Department of Developmental Biology of Hard Tissue, Hokkaido University Graduate School of Dental Medicine, Kita 13 Nishi 7, Kita-ku, Sapporo, 060-8586, Japan

    Tsuneyuki Yamamoto, Tomomaya Yamamoto, Tamaki Yamada, Tomoka Hasegawa, Hiromi Hongo & Norio Amizuka

  2. Division of Biochemistry, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Kimimitsu Oda

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  1. Tsuneyuki Yamamoto
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  2. Tomomaya Yamamoto
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  3. Tamaki Yamada
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  4. Tomoka Hasegawa
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  5. Hiromi Hongo
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  6. Kimimitsu Oda
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  7. Norio Amizuka
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Correspondence to Tsuneyuki Yamamoto.

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Yamamoto, T., Yamamoto, T., Yamada, T. et al. Hertwig’s epithelial root sheath cell behavior during initial acellular cementogenesis in rat molars. Histochem Cell Biol 142, 489–496 (2014). https://doi.org/10.1007/s00418-014-1230-1

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