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Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation

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Abstract

Ca2+ sensitivity of smooth muscle (SM) contraction is determined by CPI-17, an inhibitor protein for myosin light chain phosphatase (MLCP). CPI-17 is highly expressed in mature SM cells, but the expression level varies under pathological conditions. Here, we determined the expression of CPI-17 in embryonic SM tissues and arterial neointimal lesions using immunohistochemistry. As seen in adult animals, the predominant expression of CPI-17 was detected at SM tissues on mouse embryonic sections, whereas MLCP was ubiquitously expressed. Compared with SM α-actin, CPI-17 expression doubled in arterial SM from embryonic day E10 to E14. Like SM α-actin and other SM marker proteins, CPI-17 was expressed in embryonic heart, and the expression was down-regulated at E17. In adult rat, CPI-17 expression level was reduced to 30% in the neointima of injured rat aorta, compared with the SM layers, whereas the expression of MLCP was unchanged in both regions. Unlike other SM proteins, CPI-17 was detected at non-SM organs in the mouse embryo, such as embryonic neurons and epithelium. Thus, CPI-17 expression is reversibly controlled in response to the phenotype transition of SM cells that restricts the signal to differentiated SM cells and particular cell types.

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Acknowledgments

We thank Dr. Marion J. Siegman for critical reading and valuable discussions, and Dr. Shiu-Ying Ho for the technical support. This work was supported by grants from NHLBI HL83261, PA C.U.R.E. grant (to M.E.), NHLBI HL48807 (to M.E. and A.V.S.), and Korea Research Foundation (KRF-2007-357-E00004) funded by Korean Government (MOEHRD) (to J.I.K.)

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Authors and Affiliations

  1. Department of Molecular Physiology and Biophysics, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA

    Jee In Kim, Garbo D. Young & Masumi Eto

  2. Department of Molecular Physiology and Biological Physics, The University of Virginia, 1300 Jefferson Park Avenue, Charlottesville, VA, 22908, USA

    Li Jin & Avril V. Somlyo

  3. Kimmel Cancer Center, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA

    Masumi Eto

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  1. Jee In Kim
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  2. Garbo D. Young
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  3. Li Jin
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  4. Avril V. Somlyo
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  5. Masumi Eto
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Correspondence to Masumi Eto.

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Kim, J.I., Young, G.D., Jin, L. et al. Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation. Histochem Cell Biol 132, 191–198 (2009). https://doi.org/10.1007/s00418-009-0604-2

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  • DOI: https://doi.org/10.1007/s00418-009-0604-2

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