Changes of TGF-β2, MMP-2, and TIMP-2 levels in the vitreous of patients with high myopia

  • Hong Zhuang
  • Rong Zhang
  • Qinmeng Shu
  • Rui Jiang
  • Qing Chang
  • Xin Huang
  • Chunhui Jiang
  • Gezhi Xu
Basic Science

DOI: 10.1007/s00417-014-2768-2

Cite this article as:
Zhuang, H., Zhang, R., Shu, Q. et al. Graefes Arch Clin Exp Ophthalmol (2014) 252: 1763. doi:10.1007/s00417-014-2768-2

Abstract

Purpose

To investigate the concentrations of transforming growth factor (TGF)-β2, matrix metalloproteinase (MMP)-2, and tissue inhibitor of metalloproteinase (TIMP)-2 in the vitreous of patients with high myopia.

Methods

Twenty-six patients with high myopia (HM) who received vitrectomy for macular retinoschisis or macular hole were enrolled in this prospective study. Twenty-six patients with idiopathic macular hole or macular epiretinal membrane were chosen as a control group. Vitreous samples were obtained during the vitrectomy surgery. The levels of TGF-β2、MMP-2、TIMP-2 in the vitreous samples were measured by enzyme-linked immunosorbent assay. The MMP activity was determined by a fluorometric assay.

Results

There was no significant difference in the vitreous level of TGF-β2 between HM (1.64 ± 0.38 ng/ml) and the control group (1.56 ± 0.32 ng/ml, p = 0.56). The vitreous levels of MMP-2 in HM (32.40 ± 14.90 ng/ml) were significantly higher than in the control group (21.42 ± 6.74 ng/ml, p < 0.01). The ratio of MMP-2/TIMP-2 was significantly elevated in the vitreous samples from HM (0.61 ± 0.19), compared to the control group (0.48 ± 0.11, p < 0.05). The MMP activity was also significantly elevated in the vitreous samples from HM (4,030.8 ± 1,257.3 FIU), compared to the control group (3,245.8 ± 835.6 FIU, p < 0.05).

Conclusions

The elevated MMP/TIMP ratio and MMP activity may play a role in the pathogenesis of human high myopia. Large prospective studies are needed to further investigate the effect of MMPs in the pathogenesis of human high myopia.

Keywords

Transforming growth factor β2Matrix metalloproteinase 2Tissue inhibitor of metalloproteinase 2VitreousHigh myopia

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Hong Zhuang
    • 1
    • 3
  • Rong Zhang
    • 2
    • 3
  • Qinmeng Shu
    • 1
    • 3
  • Rui Jiang
    • 1
  • Qing Chang
    • 1
  • Xin Huang
    • 1
  • Chunhui Jiang
    • 1
  • Gezhi Xu
    • 1
    • 3
  1. 1.Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical CollegeFudan UniversityShanghaiChina
  2. 2.Research Center, Eye and ENT Hospital, Shanghai Medical CollegeFudan UniversityShanghaiChina
  3. 3.Shanghai Key Laboratory of Visual Impairment and RestorationFudan UniversityShanghaiChina