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May glutathione S-transferase M1 positive genotype afford protection against primary open-angle glaucoma?

  • Clinical Investigation
  • Published:
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Abstract

Purpose

To find out whether the polymorphism at GSTM1, GSTT1 and GSTP1 loci is associated with increased susceptibility to glaucoma.

Methods

We genotyped 153 primary open angle patients and 159 healthy controls. Genomic DNA from peripheral blood was examined using polymerase chain reaction and defined for the genetic polymorphisms of glutathione S-transferase.

Results

The frequency of the GSTM1 null genotype individuals among the glaucoma patients was significanlty higher than in controls (54.9 vs 40.9%) with odds ratio of 1.64 (95% CI: 1.10–2.59). The frequency of the GSTT1 and GSTP1 in both groups were not statistically different.

Conclusion

The present study suggests that the GSTM1 null genotype may be a genetic risk factor for development of primary open angle glaucoma. Further associations studies in other polymorphic genes for xenobiotic–metabolizing enzymes are needed to elucidate the environmental-genetic interaction in the underlying cause of primary open angle glaucoma.

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References

  1. Alward WL, Fingert JH, Coote MA, Johnson AT, Lerner SF, Junqua D, Durcan FJ, McCartney PJ, Mackey DA, Sheffield VC, Stone EM (1998) Clinical features associated with mutations in the choromosome 1 open-angle glaucoma gene (GLC1A). N Engl J Med 338:1022–1027

    Article  CAS  PubMed  Google Scholar 

  2. Augusteyn RC (1981) Protein modification in cataract: possible oxidative mechanisms. In: Duncan G (ed) Mechanisms of cataract formation in the human lens. Academic, London, pp 71–116

    Google Scholar 

  3. Beatty S, Koh H, Phil M, Henson D, Boulton M (2000) The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv Ophthalmol 45:115–134

    Article  CAS  PubMed  Google Scholar 

  4. Behndig A, Svensson B, Marklund SL, Karlsson K (1998) Superoxide dismutase isoenzymes in the human eye. Invest Ophthalmol Vis Sci 39:471–475

    CAS  PubMed  Google Scholar 

  5. Berman ER (1991) Biochemistry of the eye. Plenum, New York

    Google Scholar 

  6. Board PG, Baker RT, Chelvanayagama G, Jermiin L (1997) Zeta, a novel class of glutathione transferases in a range of species from plants to humans. Biochem J 328:929–935

    CAS  PubMed  Google Scholar 

  7. Çamdeviren H, Ozkan M, Baspinar E (2003) Biyoistatistik el kitabi. In: Çamdeviren H (ed) Tibbi Araştirma Tipleri ve Sebep-Sonuç Ilişkileri. Lojistik Regresyon Analizi, Fersa Matbaacilik Ankara, pp 88–94

    Google Scholar 

  8. Coca PM, Escribano J, Ortego J (1999) Differential gene expression in the human ciliary epithelium. Prog Retin Eye Res 18:403–429

    Article  PubMed  Google Scholar 

  9. Deakin M, Elder J, Hendrickse C, Peckham D, Baldwin D, Pantin C, Wild N, Leopard P, Bell DA, Jones P, Duncan H, Branigan K, Alldersea J, Fryer AA, Strange RC (1996) Glutathione S-transferase GSTT1 genotypes and susceptibility to cancer: studies of interactions with GSTM1 in lung, oral, gastric and colorectal cancers. Carcinogenesis 17:881–884

    CAS  PubMed  Google Scholar 

  10. Duncan H, Swan CH, Green J, Jones P, Brannigan K, Alldersea J, Fryer AA, Strange R (1995) Susceptibility to ulcerative colitis and Crohn’s disease: interaction between glutathione S-transferase GSTM1 and GSTT1 genotypes. Clin Chim Acta 240:53–61

    Article  CAS  PubMed  Google Scholar 

  11. Fingert JH, Heon E, Liebmann JM, Yamamoto T, Craig JE, Rait J, Kawase K, Hoh ST, Buys YM, Dickinson J, Hockey RR, Williams-Lyn D, Trope G, Kitazawa Y, Ritch R, Mackey DA, Alward WL, Sheffield VC, Stone EM (1999) Analysis of myocilin mutations in 1703 glaucoma patients from five different populations. Hum Mol Genet 8:899–905

    Article  CAS  PubMed  Google Scholar 

  12. Garte S, Gaspari L, Alexandrie AK, Ambrosone C, Autrup H, Autrup JL, Baranova H, Bathum L, Benhamou S, Boffetta P, Bouchardy C, Breskvar K, Brockmoller J, Cascorbi I, Clapper ML, Coutelle C, Daly A, Dell’Omo M, Dolzan V, Dresler CM, Fryer A, Haugen A, Hein DW, Hildesheim A, Hirvonen A, Hsieh LL, Ingelman-Sundberg M, Kalina I, Kang D, Kihara M, Kiyohara C, Kremers P, Lazarus P, Le Marchand L, Lechner MC, van Lieshout EM, London S, Manni JJ, Maugard CM, Morita S, Nazar-Stewart V, Noda K, Oda Y, Parl FF, Pastorelli R, Persson I, Peters WH, Rannug A, Rebbeck T, Risch A, Roelandt L, Romkes M, Ryberg D, Salagovic J, Schoket B, Seidegard J, Shields PG, Sim E, Sinnet D, Strange RC, Stucker I, Sugimura H, To-Figueras J, Vineis P, Yu MC, Taioli E (2001) Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomarkers Prev 10:1239–1248

    CAS  PubMed  Google Scholar 

  13. Goldberg I (2000) How common is glaucoma worldwide? In: Weinreb RN, Kitazawa Y, Krieglstein GK (eds) Glaucoma in the 21st Century Harcourt Health Communications, Barcelona, pp 3–8

  14. Hayes JD, Pulford DJ (1995) The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 30:445–600

    CAS  PubMed  Google Scholar 

  15. Hayes JD, Strange RC (1995) Potential contribution of the glutathione S-transferase supergene family to resistance to oxidative stress. Free Radic Res 22:193–207

    CAS  PubMed  Google Scholar 

  16. Hiller R, Kahn HA (1975) Blindness from glaucoma. Am J Ophthalmol 80:62–69

    CAS  PubMed  Google Scholar 

  17. Izzotti A, Sacca SC, Cartiglio C, De Flora S (2003) Oxidative deoxyribonucleic acid damage in the eyes of glaucoma patients. Am J Med 114:638–646

    Article  CAS  PubMed  Google Scholar 

  18. Jahsson M, Rada A, Tomic L, Larsson LI, Wadelius C (2003) Analysis of the glutathione S-transferase M1 gene using pyrosequencing and multiplex PCR-no evidence of association to glaucoma. Exp Eye Res 77:239–243

    Article  PubMed  Google Scholar 

  19. Juronen E, Tasa G, Veromann S, Parts L, Tiddla A, Pulges R, Panov A, Soovere L, Koka K, Mikelsaar AV (2000) Polymorphic glutathione S-tranferase M1 is a risk factor of primary open-angle glaucoma among Estonians. Exp Eye Res 71:447–452

    Article  CAS  PubMed  Google Scholar 

  20. Kato S, Bowrnan ED, Harrington AM, Blömeke B, Shields PG (1995) Human lung carcinogen-DNA adduct levels mediated by genetic polymorphisms in vivo. J Natl Cancer Res 87:902–907

    CAS  Google Scholar 

  21. Ketterer B, Meyer DJ, Taylor JB, Pemble S, Coles B, Fraser G (1990) GSTs and protection against oxidative stress. In: Hayes JD, Pickett CB, Mantle TJ (eds) Glutathione S-transferases and drug resistance. Taylor & Francis, London, pp 97–109

    Google Scholar 

  22. Ko Y, Koch B, Harth V, Schinidis A, Thier R, Vetter H, Bolt HM, Brüning T (2000) Rapid analysis of GSTM1, GSTT1 and GSTP1 polymorphisms using real-time polymerase chain reaction. Pharmacogenetics 10:1–4

    Article  Google Scholar 

  23. Lafuente A, Pujol F, Carretero P, Villa JP, Cuchi A (1993) Human glutathione S-transferase μ (GSTμ) deficiency as a marker for the susceptibility to bladder and larynx cancer among smokers. Cancer Lett 68:49–54

    Article  CAS  PubMed  Google Scholar 

  24. Mannervik B, Awasthi YC, Board PG, Hayes JD, Di Ilio C, Ketterer B, Listowsky I, Morgenstern R, Muramatsu M, Pearson WR, Pickett CB, Sato K, Widersten M, Wolf CR (1992) Nomenclature for human glutathione transferases. Biochem J 282:305–308

    CAS  PubMed  Google Scholar 

  25. McWilliams JE, Sanderson BJS, Harris EL, Richert-Boe KE, Henner WD (1995) Glutathione S-transferase M1 (GSTM1) deficiency and lung cancer risk. Cancer Epidemiol Biomarkers Prev 4:589–594

    CAS  PubMed  Google Scholar 

  26. Meyer J, Coles B, Pemble SE, Gilmore KS, Fraser GM, Ketterer B (1991) Theta, a new class of glutathione transferases purified from rat and man. Biochem J 274:409–414

    CAS  PubMed  Google Scholar 

  27. Morita S, Yano M, Tsujinaka T, Akiyama Y, Taniguchi M, Kaneko K, Miki H, Fujii T, Yoshino K, Kusuoka H, Monden M (1999) Genetic polymorphism of drug-metabolizing enzymes and susceptibility to head and neck squamous cell carcinoma. Int J Cancer 80:685–688

    Article  CAS  PubMed  Google Scholar 

  28. Nguyen KP, Weiss H, Karageuzian LN (1985) Glutathione reductase of calf trabecular meshwork. Invest Ophthalmol Vis Sci 26:887–890

    CAS  PubMed  Google Scholar 

  29. Pemble S, Schroeder KR, Spencer SR, Meyer DJ, Hallier E, Bolt HM, Ketterer B, Taylor JB (1994) Human glutathione S-transferase (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem J 300:271–276

    CAS  PubMed  Google Scholar 

  30. Quigley HA (1998) The search for glaucoma genes-implications for pathogenesis and disease detection. N Engl J Med 338:1063–1064

    Article  CAS  PubMed  Google Scholar 

  31. Raymond V (1997) Molecular genetics of the glaucomas: mapping of the first five “GLC” loci. Am J Hum Genet 60:272–277

    CAS  PubMed  Google Scholar 

  32. Ryberg D, Skaug V, Hewer A, Phillips DH, Harries LW, Wolf CR, Ogreid D, Ulvik A, Vu P, Haugen A (1997) Genotypes of glutathione transferase M1 and P1 and their significance for lung DNA adduct levels and cancer risk. Carcinogenesis 18:1285–1289

    Article  CAS  PubMed  Google Scholar 

  33. Seidegard J, Pero RW (1988) The genetic variation and the expression of human glutathione transferase mu. Klin Wochenschr 66:25–26

    Google Scholar 

  34. Sekine Y, Hommura S, Harada S (1995) Frequency of glutathione S-tranferase 1 gene deletion and its possible correlation with cataract formation. Exp Eye Res 60:159–163

    CAS  PubMed  Google Scholar 

  35. Stewart WC (1995) The effect of lifestyle on the relative risk to develop open-angle glaucoma. Curr Opin Ophthalmol 6:3–9

    CAS  Google Scholar 

  36. Stone EM, Fingert JH, Alward WL, Nguyen TD, Polansky JR, Sunden SL, Nishimura D, Clark AF, Nystuen A, Nichols BE, Mackey DA, Ritch R, Kalenak JW, Craven ER, Sheffield VC (1997) Identification of a gene that causes primary open angle glaucoma. Science 275:668–670

    Google Scholar 

  37. Watson MA, Stewart RK, Smith GB, Massey TE, Bell DA (1998) Human glutathione S-transferase P1 polymorphism: relationship to lung tissue enzyme activity and population frequency distribution. Carcinogenesis 19:275–280

    Article  CAS  PubMed  Google Scholar 

  38. Wilson R, Martone J (1996) Epidemiology of chronic open-angle glaucoma. In: Ritch R, Shields M, Krupin T (eds) The glaucomas. Mosby, St Louis, pp 753–768

    Google Scholar 

  39. Wolfs RCW, Klaver CW, Ramrattan RS, Van Dujin CM, Hofman A, de Jong PTVM (1998) Genetic risk of primary open angle glaucoma. Arch Ophthalmol 116:1640–1645

    CAS  PubMed  Google Scholar 

  40. Yang J, Tezel G, Patil RV, Romano C, Wax MB (2001) Serum autoantibody against glutathione S-transferase in patients with glaucoma. Invest Ophthalmol Vis Sci 42:1273–1276

    CAS  PubMed  Google Scholar 

  41. Zimniak P, Nanduri B, Pikula S, Bandorowicz-Pikula J, Singhal SS, Srivastava SK, Awasthi S, Awasthi YC (1994) Naturally occurring human glutathione S-transferase GSTP1-1 isoforms with isoleucin and valine in position 104 differ in enzymic properties. Eur J Biochem 224:893–899

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Ophthalmology, Mersin Üniversitesi Tip Fakültesi, Mersin, Turkey

    Özlem Yildirim, Özay Öz & Ayça Yilmaz

  2. Department of Medical Biology and Genetic, Mersin Üniversitesi Tip Fakültesi, Mersin, Turkey

    Nurcan Aras Ateş

  3. Department of Biochemistry, Mersin Üniversitesi Tip Fakültesi, Mersin, Turkey

    Lülüfer Tamer & Uĝur Atik

  4. Department of Biostatistics, Mersin Üniversitesi Tip Fakültesi, Mersin, Turkey

    Handan Çamdeviren

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  1. Özlem Yildirim
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  2. Nurcan Aras Ateş
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Correspondence to Özlem Yildirim.

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Yildirim, Ö., Ateş, N.A., Tamer, L. et al. May glutathione S-transferase M1 positive genotype afford protection against primary open-angle glaucoma?. Graefe's Arch Clin Exp Ophthalmol 243, 327–333 (2005). https://doi.org/10.1007/s00417-004-1013-9

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  • DOI: https://doi.org/10.1007/s00417-004-1013-9

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