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Micro-CT features of intermediate gunshot wounds covered by textiles

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Abstract

The analysis of gunshot residue (GSR) on the clothing and the underlying skin of the victim may play an important role in the reconstruction of the shooting incident. The aim of the present study was to test micro-computed tomography (micro-CT) for the analysis of firearm wounds experimentally produced on human skin covered by textiles. Firing trials were performed on 60 sections of human calves enveloped by a single layer of fabric (cotton or jeans or leather or nylon) and 15 controls consisting of bare calves. Experimental firings were conducted in a ballistic laboratory at three different muzzle-to-target distances (5, 15, and 30 cm), using a .32 ACP pistol (Beretta Mod. 81) loaded with full-jacketed bullets coming from the same production lot (7.65 × 17 mm, Browning SR). The visual inspection revealed the classic pattern of GSR distribution on the fabrics and the skin of control samples, while only a dark ring around the entrance lesion was identified on the skin beneath the fabrics. Micro-CT analysis showed the presence of radiopaque material on all entrance wounds, with a statistically significant difference between cases and controls. No differences were found among specimens covered by fabrics, with regard to the firing distance and the type of clothing. No GSR-like deposits were detected in exit wounds. Our results suggest that micro-CT analysis may be a useful screening tool for differentiating entry from exit gunshot wounds when the covering textiles are contaminated, damaged, or missing.

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References

  1. Karger B, Billeb E, Koops E, Brinkmann B (2002) Autopsy features relevant for discrimination between suicidal and homicidal gunshot injuries. Int J Leg Med 116:273–278

    Article  CAS  Google Scholar 

  2. Grosse Perdekamp M, Nadjem H, Merkel J, Braunwarth R, Pollak S, Thierauf A (2011) Two-gun suicide by simultaneous shots to the head: interdisciplinary reconstruction on the basis of scene investigation, autopsy findings, GSR analysis and examination of firearms, bullets and cartridge cases. Int J Leg Med 125:479–485. doi:10.1007/s00414-010-0517-6

    Article  Google Scholar 

  3. Viero A, Giraudo C, Cecchetto G, Muscovich C, Favretto D, Puglisi M, Fais P, Viel G (2014) An unusual case of “dyadic-death” with a single gunshot. Forensic Sci Int 244:e1–e5. doi:10.1016/j.forsciint.2014.08.001

    Article  Google Scholar 

  4. Di Maio VJM (2015) Gunshot wounds: practical aspects of firearms, ballistics, and forensic techniques. CRC Press, Boca Raton

    Google Scholar 

  5. Vinokurov A, Zelkowicz A, Wolf EU, Zeichner A (2010) The influence of a possible contamination of the victim’s clothing by gunpowder residue on the estimation of shooting distance. Forensic Sci Int 194:72–76. doi:10.1016/j.forsciint.2009.10.011

    Article  CAS  Google Scholar 

  6. Rutty GN, Brough A, Biggs MJ, Robinson C, Lawes SD, Hainsworth SV (2013) The role of micro-computed tomography in forensic investigations. Forensic Sci Int 225:60–66. doi:10.1016/j.forsciint.2012.10.030

    Article  CAS  Google Scholar 

  7. Cecchetto G, Giraudo C, Amagliani A, Viel G, Fais P, Cavarzeran F, Feltrin G, Ferrara SD, Montisci M (2011) Estimation of the firing distance through micro-CT analysis of gunshot wounds. Int J Legal Med 125:245–251. doi:10.1007/s00414-010-0533-6

    Article  Google Scholar 

  8. Cecchetto G, Amagliani A, Giraudo C, Fais P, Cavarzeran F, Montisci M, Feltrin G, Viel G, Ferrara SD (2012) MicroCT detection of gunshot residue in fresh and decomposed firearm wounds. Int J Legal Med 126:377–383. doi:10.1007/s00414-011-0648-4

    Article  Google Scholar 

  9. Fais P, Giraudo C, Boscolo-Berto R, Amagliani A, Miotto D, Feltrin G, Viel G, Ferrara SD, Cecchetto G (2013) Micro-CT features of intermediate gunshot wounds severely damaged by fire. Int J Legal Med 127:419–425. doi:10.1007/s00414-012-0775-6

    Article  Google Scholar 

  10. Fais P, Giraudo C, Viero A, Amagliani A, Viel G, Montisci M, Miotto D, Cecchetto G (2015) Identification of bullet entrance in different type of intermediate firearm wounds through micro-computed tomography analysis. J Forensic Radiol Imag 3:147–152

    Article  Google Scholar 

  11. Dettmeyer RB, Verhoff MA, Shütz HF (2014) Forensic medicine. Fundamentals and perspective. Springer, Berlin

    Book  Google Scholar 

  12. Grosse Perdekamp M, Vennemann B, Mattern D, Serr A, Pollak S (2005) Tissue defect at the gunshot entrance wound: what happens to the skin? Int J Leg Med 119:217–222

    Article  CAS  Google Scholar 

  13. Tugcu H, Yorulmaz C, Karslioglu Y, Uner HB, Koc S, Ozdemir C, Ozaslan A, Celasun B (2006) Image analysis as an adjunct to sodium rhodizonate test in the evaluation of gunshot residues: an experimental study. Am J Forensic Med Pathol 27:296–299

    Article  Google Scholar 

  14. Carr D, Kieser J, Mabbott A, Mott C, Champion S, Girvan E (2014) Damage to apparel layers and underlying tissue due to hand-gun bullets. Int J Legal Med 128:83–93. doi:10.1007/s00414-013-0856-1

    Article  Google Scholar 

  15. Lepik D, Vasiliev V (2005) Comparison of injuries caused by the pistols Tokarev, Makarov and Glock 19 at firing distances of 10, 15 and 25cm. Forensic Sci Int 151:1–10

    Article  Google Scholar 

  16. Alakija P, Dowling GP, Gunn B (1998) Stellate clothing defects with different firearms, projectiles, ranges, and fabrics. J Forensic Sci 43:1148–1152

    Article  Google Scholar 

  17. Bailey JA, Casanova RS, Bufkin K (2006) A method for enhancing gunshot residue patterns on dark and multicolored fabrics compared with the modified Griess test. J Forensic Sci 51:812–814

    Article  Google Scholar 

  18. Zeichner A (2003) Recent developments in methods of chemical analysis in investigations of firearm-related events. Anal Bioanal Chem 376:1178–1191

    Article  CAS  Google Scholar 

  19. Sharma SP, Lahiri SC (2009) A preliminary investigation into the use of FTIR microscopy as a probe for the identification of bullet entrance holes and the distance of firing. Sci Justice 49:197–204. doi:10.1016/j.scijus.2008.07.002

    Article  CAS  Google Scholar 

  20. Berendes A, Neimke D, Schumacher R, Barth M (2006) A versatile technique for the investigation of gunshot residue patterns on fabrics and other surfaces: m-XRF. J Forensic Sci 51:1085–1090

    Article  CAS  Google Scholar 

  21. Glattstein B, Vinokurov A, Levin N, Zeichner A (2000) Improved method for shooting distance estimation. Part 1. Bullet holes in clothing items. J Forensic Sci 45:801–806

    PubMed  CAS  Google Scholar 

  22. Brazeau J, Wong RK (1997) Analysis of gunshot residues on human tissues and clothing by X-ray microfluorescence. J Forensic Sci 42:424–428

    Article  CAS  Google Scholar 

  23. Atwater CS, Durina ME, Durina JP, Blackledge RD (2006) Visualization of gunshot residue patterns on dark clothing. J Forensic Sci 51:1091–1095

    Article  Google Scholar 

  24. Bueno J, Lednev IK (2014) Attenuated total reflectance-FT-IR imaging for rapid and automated detection of gunshot residue. Anal Chem 86:3389–3396. doi:10.1021/ac4036718

    Article  CAS  Google Scholar 

  25. Kersh KL, Childers JM, Justice D, Karim G (2014) Detection of gunshot residue on dark-colored clothing prior to chemical analysis. J Forensic Sci 59:754–762. doi:10.1111/1556-4029.12409

    Article  Google Scholar 

  26. Vennemann B, Dautel F, Braunwarth R, Strassburger E, Hunzinger M, Pollak S, Grosse Perdekamp M (2008) Textile fibres along the bullet path—experimental study on a skin-gelatine composite model. Int J Legal Med 122:213–218. doi:10.1007/s00414-007-0221-3

    Article  CAS  Google Scholar 

  27. Grosse Perdekamp M, Kneubuehl BP, Serr A, Vennemann B, Pollak S (2006) Gunshot-related transport of micro-organisms from the skin of the entrance region into the bullet path. Int J Legal Med 120:257–264

    Article  CAS  Google Scholar 

  28. Vennemann B, Grosse Perdekamp M, Kneubuehl BP, Serr A, Pollak S (2006) Gunshot-related displacement of skin particles and bacteria from the exit region back into the bullet path. Int J Legal Med 121:105–111

    Article  Google Scholar 

  29. Kieser DC, Carr DJ, Girvan L, Leclair SC, Horsfall I, Theis JC, Swain MV, Kieser JA (2013) Identifying the source of bullet wipe: a randomised blind trial. Int J Legal Med 127:951–955. doi:10.1007/s00414-013-0874-z

    Article  CAS  Google Scholar 

  30. Amadasi A, Brandone A, Rizzi A, Mazzarelli D, Cattaneo C (2012) The survival of metallic residues from gunshot wounds in cremated bone: a SEM-EDX study. Int J Legal Med 126:525–531. doi:10.1007/s00414-011-0661-7

    Article  Google Scholar 

  31. Viel G, Cecchetto G, Fabbri LD, Furlan C, Ferrara SD, Montisci M (2009) Forensic application of ESEM and XRF-EDS techniques to a fatal case of sodium phosphate enema intoxication. Int J Legal Med 123:345–350. doi:10.1007/s00414-009-0344-9

    Article  CAS  Google Scholar 

  32. Udey RN, Hunter BC, Smith RW (2011) Differentiation of bullet type based on the analysis of gunshot residue using inductively coupled plasma mass spectrometry. J Forensic Sci 56:1268–1276. doi:10.1111/j.1556-4029.2011.01836.x

    Article  CAS  Google Scholar 

  33. Poppa P, Porta D, Gibelli D, Mazzucchi A, Brandone A, Grandi M, Cattaneo C (2011) Detection of blunt, sharp force and gunshot lesions on burnt remains: a cautionary note. Am J Forensic Med Pathol 32:275–279. doi:10.1097/PAF.0b013e3182198761

    Article  Google Scholar 

  34. Gibelli D, Brandone A, Andreola S, Porta D, Giudici E, Grandi MA, Cattaneo C (2010) Macroscopic, microscopic, and chemical assessment of gunshot lesions on decomposed pig skin. J Forensic Sci 55:1092–1097. doi:10.1111/j.1556-4029.2010.01378.x

    Article  Google Scholar 

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Correspondence to Giovanni Cecchetto.

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The study was approved by the Ethical Committee of the University Hospital of Padova (protocol no. 2013P).

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Giraudo, C., Fais, P., Pelletti, G. et al. Micro-CT features of intermediate gunshot wounds covered by textiles. Int J Legal Med 130, 1257–1264 (2016). https://doi.org/10.1007/s00414-016-1403-7

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  • DOI: https://doi.org/10.1007/s00414-016-1403-7

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