Radiation and Environmental Biophysics

, Volume 52, Issue 3, pp 375–387

Effects of gamma radiation on hard dental tissues of albino rats: investigation by light microscopy

  • Nabil El-Faramawy
  • Reham Ameen
  • Khaled El-Haddad
  • Medhat El-Zainy
Original Paper

DOI: 10.1007/s00411-013-0469-6

Cite this article as:
El-Faramawy, N., Ameen, R., El-Haddad, K. et al. Radiat Environ Biophys (2013) 52: 375. doi:10.1007/s00411-013-0469-6

Abstract

The present work aims at studying the effect of gamma radiation on the hard dental tissues. Eighty adult male albino rats with weights of about 250 g were used. The rats were irradiated at 0.2, 0.5, 1.0, 2.0, 4.0 and 6.0 Gy whole-body gamma doses. The effects on hard dental tissue samples were investigated after 48 h in histological and ground sections using light microscopy. Areas of acid phosphatase activity were detected using tartrate-resistant acid phosphatase (TRAP) stains. Observation of histological sections revealed disturbance in predentin thickness and odontoblastic layer as the irradiation dose increased. In cementum, widened cementocytes lacunae were occasionally detected even with low irradiated doses. On the other hand, relatively homogenous enamel was detected with darkened areas in enamel surface at doses over than 0.5 Gy. TRAP-positive cells were detected on the surface of the dentin of irradiated groups as well as cementum surface. Minimal detectable changes were observed in ground sections.

Keywords

Gamma radiationDental tissuesLight microscopeDental histology

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Nabil El-Faramawy
    • 1
  • Reham Ameen
    • 2
  • Khaled El-Haddad
    • 2
  • Medhat El-Zainy
    • 2
  1. 1.Physics Department, Faculty of ScienceAin Shams UniversityAbbassia, CairoEgypt
  2. 2.Oral Biology Department, Faculty of DentistryAin Shams UniversityCairoEgypt