Skip to main content

Advertisement

SpringerLink
Log in

In Situ Evidence of Pulmonary Endothelial Activation in Patients with Granulomatosis with Polyangiitis and Systemic Sclerosis

  • Published:
Lung Aims and scope Submit manuscript

Abstract

Introduction

The aim of this study was to investigate the in situ pulmonary endothelial activation in lung lesions of granulomatosis with polyangiitis (GPA) and systemic sclerosis (SScl).

Methods

We examined the endothelial expression of ICAM-1, VCAM-1, and E-selectin using immunohistochemistry on formalin-fixed, paraffin-embedded sections of lung lesions of GPA, interstitial lung disease associated with SScl and controls.

Results

A significantly enhanced expression of ICAM-1 and E-selectin was observed in GPA and SScl pulmonary endothelium compared to controls. VCAM-1 was more pronouncedly expressed in GPA compared to SScl.

Conclusion

These observations are an evidence of in situ pulmonary vascular endothelial activation in lesions of GPA and SScl, adding information to the pathogenic knowledge of both diseases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Katsumoto TR, Whitfield ML, Connolly MK (2011) The pathogenesis of systemic sclerosis. Annu Rev Pathol 6:509–537. doi:10.1146/annurev-pathol-011110-130312

    Article  CAS  PubMed  Google Scholar 

  2. Kallenberg CG, Heeringa P, Stegeman CA (2006) Mechanisms of disease: pathogenesis and treatment of ANCA-associated vasculitides. Nat Clin Pract Rheumatol 2(12):661–670

    Article  CAS  PubMed  Google Scholar 

  3. Jonker ND, Peters AM, Gaskin G et al (1992) A retrospective study of radiolabeled granulocyte kinetics in patients with systemic vasculitis. J Nucl Med 33(4):491–497

    CAS  PubMed  Google Scholar 

  4. Arrizabalaga P, Solé M, Abellana R et al (2008) Renal expression of adhesion molecules in anca-associated disease. J Clin Immunol 28(5):411–419. doi:10.1007/s10875-008-9215-2

    Article  CAS  PubMed  Google Scholar 

  5. Mayet WJ, Schwarting A, Orth T et al (1996) Antibodies to proteinase 3 mediate expression of vascular cell adhesion molecule-1 (VCAM-1). Clin Exp Immunol 103(2):259–267

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. De Bandt M, Meyer O, Hakim J et al (1997) Antibodies to proteinase-3 mediate expression of intercellular adhesion molecule-1 (ICAM-1, CD 54). Br J Rheumatol 36(8):839–846

    Article  PubMed  Google Scholar 

  7. Koch AE, Kronfeld-Harrington LB, Szekanecz Z et al (1993) In situ expression of cytokines and cellular adhesion molecules in the skin of patients with systemic sclerosis. Their role in early and late disease. Pathobiology 61(5–6):239–246

    Article  CAS  PubMed  Google Scholar 

  8. Claman HN, Giorno RC, Seibold JR (1991) Endothelial and fibroblastic activation in scleroderma. The myth of the “uninvolved skin”. Arthritis Rheum 34(12):1495–1501

    Article  CAS  PubMed  Google Scholar 

  9. Tognarelli S, Gayet J, Lambert M et al (2014) Tissue-specific microvascular endothelial cells show distinct capacity to activate NK cells: implications for the pathophysiology of granulomatosis with polyangiitis. J Immunol 192(7):3399–3408. doi:10.4049/jimmunol.1301508

    Article  CAS  PubMed  Google Scholar 

  10. Leavitt RYJC, Fauci AS, Bloch DA et al (1990) The American College of Rheumatology 1990 criteria for the classification of Wegener’s granulomatosis. Arthritis Rheum 33(8):1101

    Article  CAS  PubMed  Google Scholar 

  11. van den Hoogen F, Khanna D, Fransen J et al (2013) 2013 classification criteria for systemic sclerosis: an American College of Rheumatology/European League against Rheumatism collaborative initiative. Arthritis Rheum 65(11):2737–2747. doi:10.1002/art.38098

    Article  PubMed Central  PubMed  Google Scholar 

  12. Katzenstein A-L (2006) Katzenstein and Askin´s surgical lung pathology of non-neoplastic lung disease, 4th edn. Saunders Elsevier, Philadelphia

    Google Scholar 

  13. Gundersen HJ, Bendtsen TF, Korbo L et al (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96(5):379–394

    Article  CAS  PubMed  Google Scholar 

  14. Carlos TM, Harlan JM (1994) Leukocyte-endothelial adhesion molecules. Blood 84(7):2068–2101

    CAS  PubMed  Google Scholar 

  15. Franco de Carvalho E, Parra ER, de Souza R et al (2008) Parenchymal and vascular interactions in the pathogenesis of nonspecific interstitial pneumonia in systemic sclerosis and idiopathic interstitial pneumonia. Respiration 76(2):146–153

    Article  PubMed  Google Scholar 

  16. Iannone F, Riccardi MT, Guiducci S et al (2008) Bosentan regulates the expression of adhesion molecules on circulating T cells and serum soluble adhesion molecules in systemic sclerosis-associated pulmonary arterial hypertension. Ann Rheum Dis 67(8):1121–1126

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We are particularly grateful for the assistance given by Esmeralda Miristeni Eher and Sandra de Morais Fernezlian with the immunohistochemical procedures. We also wish to acknowledge the help provided by Maristela Peres Rangel and Bruno Guedes Baldi in data acquisition and data analysis, respectively.

Conflict of interest

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Pulmonary Division, Heart Institute (InCor), University of São Paulo Medical School, Av Dr Eneas de Carvalho Aguiar, 44 – 5º andar, Bloco II (secretaria de pneumologia), São Paulo, SP, 05403-900, Brazil

    Leticia Kawano-Dourado, Carla Valeri & Carmen Sílvia Valente Barbas

  2. Pathology Division, University of São Paulo Medical School, Av. Dr. Arnaldo, 455 - Cerqueira César, São Paulo, SP, 01246-903, Brazil

    Alexandre M. Ab’Saber & Vera Luiza Capelozzi

Authors
  1. Leticia Kawano-Dourado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alexandre M. Ab’Saber
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Vera Luiza Capelozzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Carla Valeri
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carmen Sílvia Valente Barbas
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Leticia Kawano-Dourado.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kawano-Dourado, L., Ab’Saber, A.M., Capelozzi, V.L. et al. In Situ Evidence of Pulmonary Endothelial Activation in Patients with Granulomatosis with Polyangiitis and Systemic Sclerosis. Lung 193, 355–359 (2015). https://doi.org/10.1007/s00408-015-9718-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00408-015-9718-6

Keywords

Search

Navigation