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Driver Gene Mutations of Non-Small-Cell Lung Cancer are Rare in Primary Carcinoids of the Lung: NGS Study by Ion Torrent

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Abstract

Lung carcinoids are rare neuroendocrine tumors of the lung. Very little is known about the genetic background of these tumors. We applied Ion Torrent Ampliseq next-generation technology to study hotspot mutations of 22 lung cancer-related genes from typical and atypical lung carcinoid tumors. DNA isolated from 25 formalin-fixed, paraffin-embedded carcinoid tumors were amplified to prepare barcoded libraries covering 507 mutations included in 90 amplicons. The libraries were pooled, purified, enriched, and sequenced on ion personal genome machine. The sequences were aligned and checked for known and novel variations using Torrent Suite Software v.4.0.2. One out of 25 patients had mutations in the targeted regions sequenced. This patient had mutations in BRAF, SMAD4, PIK3CA, and KRAS. All these mutations were confirmed as somatic and are previously known mutations. In summary, mutations in genes commonly mutated in non-small-cell lung cancer are not common in lung carcinoids.

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Acknowledgments

The authors thank Tiina Wirtanen for her assistance in sequencing reactions. The study was supported by Sigrid Jusélius Foundation, Cancer Society of Finland, and The Finnish Work Environment Fund.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical Approval and Informed Consent

All procedures performed in these studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. Unit of Biological Anthropology, Department of Animal Biology, Plant Biology and Ecology, Universitat Autònoma de Barcelona, Barcelona, Catalonia, Spain

    Gemma Armengol

  2. Department of Pathology, Haartman Institute, University of Helsinki, Haartmaninkatu 3, P.O.Box PL 21, 00014, Helsinki, Finland

    Gemma Armengol, Virinder Kaur Sarhadi, Milja Tikkanen & Sakari Knuutila

  3. Department of Pathology, HUSLAB, Helsinki University Central Hospital, Helsinki, Finland

    Mikko Rönty

  4. Department of Pulmonary Medicine, Heart and Lung Center, Helsinki University Central Hospital, Helsinki, Finland

    Aija Knuuttila

Authors
  1. Gemma Armengol
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  2. Virinder Kaur Sarhadi
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  3. Mikko Rönty
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  4. Milja Tikkanen
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  5. Aija Knuuttila
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  6. Sakari Knuutila
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Corresponding author

Correspondence to Sakari Knuutila.

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Armengol, G., Sarhadi, V.K., Rönty, M. et al. Driver Gene Mutations of Non-Small-Cell Lung Cancer are Rare in Primary Carcinoids of the Lung: NGS Study by Ion Torrent. Lung 193, 303–308 (2015). https://doi.org/10.1007/s00408-015-9690-1

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  • DOI: https://doi.org/10.1007/s00408-015-9690-1

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