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Impact of microsatellite alteration in surgical margins on local recurrence in oral cavity cancer patients

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Abstract

The aim of this study was to investigate the association between microsatellite alteration in the surgical margins and local recurrence of oral cavity squamous cell carcinoma patients. Surgical specimens confirmed by pathological examination and corresponding surgical margins were collected from 120 oral cavity squamous cell carcinoma patients. Ten microsatellite markers were examined in the tumor specimens and paired surgical margins, which proved to be negative on pathological assessment. The specimens and surgical margins were amplified by polymerase chain reaction followed by computerized analysis. Forty-two specimens (35.0 %) with microsatellite instability (MSI) in at least one marker were found, and more than half of the specimens (n = 73, 60.8 %) had loss of heterozygosity (LOH) in at least one marker. Although MSI and LOH were not associated with the prognosis of oral cavity squamous cell carcinoma patients, presence of MSI in the tumor-free surgical margins increased the risk of local recurrence (hazard ratio: 9.549; 95 % confidence interval: 4.143–22.01). Genetic analysis of tumor-free surgical margins is a useful tool for identifying oral cavity squamous cell carcinoma patients who are vulnerable to local recurrence.

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Acknowledgments

The authors thank the Biostatistics Task Force of Taichung Veterans General Hospital for assisting with the statistical analysis.

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Authors and Affiliations

  1. Department of Radiation Oncology, Taichung Veterans General Hospital, Taichung, Taiwan

    Jin-Ching Lin

  2. Department of Otolaryngology, Taichung Veterans General Hospital, No. 1650, Sec 4, Taiwan Boulevard, Taichung, Taiwan

    Chen-Chi Wang, Rong-San Jiang & Shih-An Liu

  3. Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan

    Jin-Ching Lin, Chen-Chi Wang & Shih-An Liu

  4. Department of Nursing, HungKuang University, Taichung, Taiwan

    Wen-Yi Wang

  5. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

    Shih-An Liu

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  1. Jin-Ching Lin
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  2. Chen-Chi Wang
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Corresponding author

Correspondence to Shih-An Liu.

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All authors declare that they have no conflict of interest.

Funding

The study was funded by the National Science Council, Taiwan, Republic of China (NSC 101-2314-B- 075A-005-MY3) and the Ministry of Health and Welfare, Taiwan, Republic of China (MOHW104-TDU-B-211-124-004).

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

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Lin, JC., Wang, CC., Jiang, RS. et al. Impact of microsatellite alteration in surgical margins on local recurrence in oral cavity cancer patients. Eur Arch Otorhinolaryngol 274, 431–439 (2017). https://doi.org/10.1007/s00405-016-4215-y

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  • DOI: https://doi.org/10.1007/s00405-016-4215-y

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