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UreA and cagA genes of Helicobacter pylori in Egyptian patients with laryngeal squamous cell carcinoma and benign laryngeal polyps: a cohort study

  • Laryngology
  • Published:
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Abstract

This work aims to estimate the prevalence of Helicobacter pylori ureA gene and evaluate cagA gene-positive strains in both patients of laryngeal squamous cell carcinoma (LSCC) and those with benign laryngeal polyps. This study included 49 patients confirmed pathologically to have LSCC and 15 patients with benign laryngeal polyps over a period from June 2013 to March 2015. Samples of laryngeal tissue were collected during direct laryngoscope under general anesthesia to be pathologically evaluated followed by analysis for H. pylori detection. Each laryngeal tissue sample was divided into three parts; one for bacteriological examination, the second for pathological examination and the third for PCR to detect both ureA and cagA genes. Out of 49 LSCC samples, 31 (64.6 %) was positive for ureA by PCR. Out of them, 29 samples (93.5 %) were cagA positive. Only three cases (20 %) of the benign laryngeal polyp were ureA positive by PCR and one of them was cagA positive by PCR. By the bacteriological culture, only eight samples (25.8 %) gave growth. All of them were ureA positive and only seven of them were cagA positive. There was a significant association between presence of H. pylori and LSCC as compared to benign laryngeal polyp which may contribute in the pathogenesis of laryngeal carcinoma. These results should be confirmed by further studies over larger number of cases.

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Abbreviations

GERD:

Gastroesophageal reflux

H. pylori :

Helicobacter pylori

LSCC:

Laryngeal squamous cell carcinoma

PCR:

Polymerase chain reaction

References

  1. Tominaga S (1999) Major avoidable risk factors of cancer. Cancer Lett 143(Suppl 1):S19–S23

    Article  CAS  PubMed  Google Scholar 

  2. Burduk PK (2006) The role of Helicobacter pylori infection in carcinoma of the larynx. Otolaryngol Pol 60(4):1–3

    Google Scholar 

  3. Grbesa I, Marinkovic M, Ivkic M, Kruslin B, Novak-Kujundzic R, Pegan B, Bogdanovic O, Bedekovic V, Gall-Troselj K (2008) Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and Helicobacter pylori infection in laryngeal squamous cell carcinoma. J Mol Med (Berl) 86(9):1057–1066

    Article  Google Scholar 

  4. Gong H, Shi Y, Zhou L, Tao L, Shi Y, Cao W, Cheng L (2012) Helicobacter pylori infection of the larynx may be an emerging risk factor for laryngeal squamous cell carcinoma. Clin Transl Oncol 14(12):905–910

    Article  PubMed  Google Scholar 

  5. Amizadeh M, Shamsadini A, Arabzadeh A, Jazayeri S (2015) Association of cagA positive Helicobacter pylori infection and laryngeal squamous cell carcinoma: a PCR approach. Indian J Otolaryngol Head Neck Surg 67(Suppl 1):51–55

    Article  PubMed  Google Scholar 

  6. Rouve P, Chakarski I, Doskov D, Dimov G, Staykova E (2005) Laryngopharyngeal symptoms and gastroesophageal reflux disease. J Voice 19:476–480

    Article  Google Scholar 

  7. Cekin E, Ozyurt M, Erkul E, Ergunay K, Cincik H, Kapucu B, Gungor A (2012) The association between Helicobacter pylori and laryngopharyngeal reflux in laryngeal pathologies. Ear Nose Throat J 91:E6–E9

    PubMed  Google Scholar 

  8. Youssef TF, Ahmed MR (2010) Treatment of clinically diagnosed laryngopharyngeal reflux disease. Arch Otolaryngol Head Neck Surg 136:1089–1092

    Article  PubMed  Google Scholar 

  9. Toros SZ, Toros AB, Yüksel OD, Ozel L, Akkaynak C, Naiboglu B (2009) Association of laryngopharyngeal manifestations and gastroesophageal reflux. Eur Arch Otorhinolaryngol 266:403–409

    Article  PubMed  Google Scholar 

  10. Forman D, Newell DG, Fullerton F, Yarnell JW, Stacey AR, Wald N, Sitas F (1991) Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation. BMJ 302(6788):1302–1305

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Eidt S, Stolte M (1995) The significance of Helicobacter pylori in relation to gastric cancer and lymphoma. Eur J Gastroenterol Hepatol 7(4):318–321

    CAS  PubMed  Google Scholar 

  12. Uemura N, Okamoto S (2000) Effect of Helicobacter pylori eradication on subsequent development of cancer after endoscopic resection of early gastric cancer in Japan. Gastroenterol Clin North Am 29(4):819–827

    Article  CAS  PubMed  Google Scholar 

  13. Patel SK, Pratap CB, Jain AK, Gulati AK, Nath G (2014) Diagnosis of Helicobacter pylori: what should be the gold standard? World J Gastroenterol 20(36):12847–12859

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Eurohepygast Study Group (2002) Risk factors for atrophic chronic gastritis in a European population: results of the Eurohepygast study. Gut 50:779–785

    Article  Google Scholar 

  15. Blaser MJ, Perez-Perez GI, Kleanthous H, Cover TL, Peek RM, Chyou PH, Stemmermann GN, Nomura A (1995) Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 55:2111–2115

    CAS  PubMed  Google Scholar 

  16. Ozyurt M, Gungor A, Ergunay K, Cekin E, Erkul E, Haznedaroglu T (2009) Real-time PCR detection of Helicobacter pylori and virulence-associated cagA in nasal polyps and laryngeal disorders. Otolaryngol Head Neck Surg 141(1):131–135

    Article  PubMed  Google Scholar 

  17. Titiz A, Ozcakir O, Ceyhan S, Yilmaz YF, Unal A, Akyon Y (2008) The presence of Helicobacter pylori in the larynx pathologies. Auris Nasus Larynx 35(4):534–538

    Article  PubMed  Google Scholar 

  18. Eshraghian A (2014) Epidemiology of Helicobacter pylori infection among the healthy population in Iran and countries of the Eastern Mediterranean Region: a systematic review of prevalence and risk factors. World J Gastroenterol 20(46):17618–17625

    Article  PubMed  PubMed Central  Google Scholar 

  19. Sabah AA, Gneidy MR, Saleh NM (2015) Prevalence of Helicobacter pylori infection among adult patients with different gastrointestinal parasites in Tanta City district. J Egypt Soc Parasitol 45(1):101–106

    Article  PubMed  Google Scholar 

  20. El-Sharouny E, El-Shazli H, Olama Z (2015) Detection of Helicobacter pylori DNA in some Egyptian water systems and its incidence of transmission to individuals. Iran J Public Health 44(2):203–210

    PubMed  PubMed Central  Google Scholar 

  21. Mohammad MA, Hussein L, Coward A, Jackson SJ (2008) Prevalence of Helicobacter pylori infection among Egyptian children: impact of social background and effect on growth. Public Health Nutr 11:230–236

    Article  PubMed  Google Scholar 

  22. Clayton CL, Kleanthous H, Coates PJ, Morgan DD, Tabaqchali S (1992) Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol 30:192–200

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Izadi F, Ahmadi A, Ghourchian S, Daneshi A, Memari F, Khadivi E, Mohammadi S (2012) Detection of helicobacter pylori in benign laryngeal lesions by polymerase chain reaction: a cross sectional study. Infect Agent Cancer 7(1):10

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Amer FA, El-Sokkary RH, Elahmady M, Gheith T, Abdelbary EH, Elnagar Y, Abdalla WM (2013) Helicobacter pylori genotypes among patients in a university hospital in Egypt: identifying the determinant of disease severity. J Microbiol Infect Dis 3:109–115

    Article  Google Scholar 

  25. Nurgalieva ZZ, Graham DY, Dahlstrom KR, Wei Q, Sturgis EM (2005) A pilot study of Helicobacter pylori infection and risk of laryngopharyngeal cancer. Head Neck 27(1):22–27

    Article  PubMed  Google Scholar 

  26. Rubin JS, Benjamin E, Prior A, Lavy J (2003) The prevalence of Helicobacter pylori infection in malignant and premalignant conditions of the head and neck. J Laryngol Otol 117(2):118–121

    Article  CAS  PubMed  Google Scholar 

  27. Masoud N, Manouchehr K, Najmeh D, Monireh H (2008) Lack of association between Helicobacter pylori and laryngeal carcinoma. Asian Pac J Cancer Prev 9(1):81–82

    PubMed  Google Scholar 

  28. Genç R, Çağlı S, Yüce İ, Vural A, Okuducu H, Patıroğlu T, Güney E (2013) The role of H. pylori in the development of laryngeal squamous cell carcinoma. Dis Markers 35(5):447–9

  29. Akbayir N, Bas¸ak T, Seven H, Sungun A, Erdem L (2005) Investigation of Helicobacter pylori colonization in laryngeal neoplasia. Eur Arch Oto-Rhino-Laryngol 262(3):170–172

    Article  Google Scholar 

  30. Batch AJG (1998) Globus pharyngeus (Part 1). J Laryngol Otol 102:152–158

    Article  Google Scholar 

  31. Paterson WG (1997) Extraesophageal complications of gastroesophageal reflux disease. Can J Gastroenterol 11:45B–50B

    Article  PubMed  Google Scholar 

  32. Glupczynski Y (1998) Microbiological and serological diagnostic tests for Helicobacter pylori: an overview. Br Med Bull 54:175–186

    Article  CAS  PubMed  Google Scholar 

  33. Kanda T, Tanaka S, Asato R, Tamaki H, Ito J, Morinaka S (2005) Investigation of Helicobacter pylori in tumor tissue specimens from patients of head and neck tumor. Practica Oto-Rhino-Laryngologica 98:571–575

    Article  Google Scholar 

  34. Burduk PK (2013) Association between infection of virulence cagA gene Helicobacter pylori and laryngeal squamous cell carcinoma. Med Sci Monit 19:584–591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Hatakayema M (2006) The role of Helicobacter pylori CagA in gastric carcinogenesis. Int J Hematol 84(4):301–308

    Article  Google Scholar 

  36. Burduk PK, Kaczmarek A, Budzynska A, Kazmierczak W, Gospodarek E (2011) Detection of Helicobacter pylori and cagA gene in nasal polyps and benign laryngeal diseases. Arch Med Res 42(8):686–689

    Article  CAS  PubMed  Google Scholar 

  37. Calvet X, Ramírez Lázaro MJ, Lehours P, Mégraud F (2013) Diagnosis and epidemiology of Helicobacter pylori infection. Helicobacter 18(Suppl 1):5–11

    Article  PubMed  Google Scholar 

  38. Yilmaz I, Erkul E, Berber U, Kucukodaci Z, Narli G, Haholu A, Demirel D (2016) The presence of Helicobacter pylori in laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 273(3):761–765

    Article  PubMed  Google Scholar 

  39. Ercan I, Cakir BO, Uzel TS, Sakiz D, Karaca C, Turgut S (2006) The role of gastric Helicobacter pylori infection in laryngopharyngeal reflux disease. Otolaryngol Head Neck Surg 135:52–55

    Article  PubMed  Google Scholar 

  40. Kizilay A, Saydam L, Aydin A, Kalcioglu MT, Ozturan O, Aydin NE (2006) Histopathologic examination for Helicobacter pylori as a possible etiopathogenic factor in laryngeal carcinoma. Chemotherapy 52(2):80–82

    Article  CAS  PubMed  Google Scholar 

  41. Islam A, Oguz H, Yucel M, Koca G, Gonultas MA, Arslan N, Demirci M (2013) Does Helicobacter pylori exist in vocal fold pathologies and in the interarytenoid region? Dysphagia 28:382–387

    Article  PubMed  Google Scholar 

  42. Siupsinskiene N, Jurgutaviciute V, Katutiene I, Janciauskas D, Vaitkus S, Damonis K (2013) Helicobacter pylori infection in laryngeal diseases. Eur Arch Otorhinolaryngol 270:2283–2288

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Ghada Barakat, Yasmin Nabiel, Omima Ali & Ghada El-Nady

  2. Otorhinolarngology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Ahmed Musaad & Asser El-Sharkawy

Authors
  1. Ghada Barakat
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  2. Yasmin Nabiel
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  3. Omima Ali
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  4. Ghada El-Nady
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  5. Ahmed Musaad
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  6. Asser El-Sharkawy
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Corresponding author

Correspondence to Ghada Barakat.

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Conflict of interest

Ghada Barakat declares that she has no conflict of interest. Yasmin Nabiel declares that she has no conflict of interest. Omaima Ali declares that she has no conflict of interest. Ghada El-Nady declares that she has no conflict of interest. Ahmed Musaad declares that he has no conflict of interest. Asser El-Sharkawy declares that he has no conflict of interest.

Ethical approval

The protocol of the study was reviewed and approved by our institutional review board. All procedures performed were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Barakat, G., Nabiel, Y., Ali, O. et al. UreA and cagA genes of Helicobacter pylori in Egyptian patients with laryngeal squamous cell carcinoma and benign laryngeal polyps: a cohort study. Eur Arch Otorhinolaryngol 273, 3243–3248 (2016). https://doi.org/10.1007/s00405-016-4114-2

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  • DOI: https://doi.org/10.1007/s00405-016-4114-2

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