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Oxidative stress in chronic otitis media

  • Otology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Chronic otitis media usually presents with a benign tumor-like lesion of the temporal bone known as a cholesteatoma. The role of oxidative stress in the pathogenesis of chronic otitis media and cholesteatoma has not yet been fully explored. Therefore, the aim of this study was to investigate the oxidative stress markers and antioxidant enzymes in patients with cholesteatomatous and noncholesteatomatous chronic otitis media and in healthy subjects. A prospective controlled trial was performed on cholesteatomatous and noncholesteatomatous chronic otitis media patients in a tertiary referral center in a university hospital. A total of 75 subjects, including 25 cholesteatomatous and 25 noncholesteatomatous chronic otitis media patients and 25 healthy subjects participated in this study. Serum total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly increased in the patient groups with or without cholesteatoma compared with the control group. Serum total antioxidant status (TAS) levels and Paraoxonase and arylesterase activity were significantly lower in the patient groups with or without cholesteatoma compared with the control group. Serum TOS and OSI levels were lower in the noncholesteatomatous group, whereas serum TAS levels were higher compared with the cholesteatomatous group. Serum arylesterase activity was significantly lower in the noncholesteatomatous group compared with the control group. The results of this study reveal that in cholesteatoma cases, the oxidative stress and antioxidant enzyme imbalance were more significant than in cases of chronic otitis media without cholesteatoma.

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References

  1. Louw L (2010) Acquired cholesteatoma pathogenesis: stepwise explanations. J Laryngol Otol 124:587–593

    Article  PubMed  Google Scholar 

  2. Olszewska E, Wagner M, Bernal-Sprekelsen M, Ebmeyer J, Dazert S, Hildmann H, Sudhoff H (2004) Etiopathogenesis of cholesteatoma. Eur Arch Otorhinolaryngol 261:6–24

    Article  PubMed  Google Scholar 

  3. Huisman MA, deHeer E, Dijke PT, Grote JJ (2007) Transforming growth factor β and wound healing in human cholesteatoma. Laryngoscope 118:94–98

    Article  Google Scholar 

  4. Taysi S, Memisogullari R, Koc M, Yazici AT, Aslankurt M, Gumustekin K, Al B, Ozabacigil F, Yilmaz A, Tahsin Ozder H (2008) Melatonin reduces oxidative stress in the rat lens due to radiation-induced oxidative injury. Int J Radiat Biol 84:803–808

    Article  PubMed  CAS  Google Scholar 

  5. Aktan B, Gundogdu C, Ucuncu H, Unal B, Sutbeyaz Y, Altas S (2004) Anti-inflammatory effect of erythromycin on histamine-induced otitis media with effusion in guinea pigs. J Laryngol Otol 118:97–101

    Article  PubMed  Google Scholar 

  6. Okumus S, Taysi S, Orkmez M, Saricicek E, Demir E, Adli M, Al B (2011) The effects of oral ginkgo biloba supplementation on radiation-induced oxidative injury in the lens of rat. Pharmacogn Mag 7:141–145

    Article  PubMed  Google Scholar 

  7. Koc S, Aksoy N, Bilinc H, Duygu F, Uysal IO, Ekinci A (2011) Paraoxonase and arylesterase activity and total oxidative/anti-oxidative status in patients with chronic adenotonsillitis. Int J Pediatr Otorhinolaryngol 75:1364–1367

    Article  PubMed  Google Scholar 

  8. Seres I, Pragh G, Deschene E, Fulop T Jr, Khalil A (2004) Study of factors influencing the decreased HDL associated PON1 activity with aging. Exp Gerontol 39:59–66

    Article  PubMed  CAS  Google Scholar 

  9. Eckerson HW, Wyte MC, La Du BN (1983) The human serum paroxonase/arylesterase polymorphism. Am J Hum Genet 35:1126–1138

    PubMed  CAS  Google Scholar 

  10. Haagen L, Brock A (1992) A new automated method for phenotyping arylesterase (E.C.3.1.1.2.) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clim Biochem 30:391–395

    CAS  Google Scholar 

  11. Myara I, Charpentier C, Lemonnier A (1982) Optimal conditions for prolidase assay by proline colorimetric determination: application to imminodipeptiduria. Clin Chim Acta 125:193–205

    Article  PubMed  CAS  Google Scholar 

  12. Chinard FP (1952) Photometric estimation of proline and ornithine. J Biol Chem 199:91–95

    PubMed  CAS  Google Scholar 

  13. Erel O (2004) A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 37:112–119

    Article  PubMed  CAS  Google Scholar 

  14. Erel O (2005) A new automated colorimetric method for measuring total oxidant status. Clin Biochem 38:1103–1111

    Article  PubMed  CAS  Google Scholar 

  15. Harma M, Harma M, Erel O (2003) Increased oxidative stress in patients with hydatidiform mole. Swiss Med Wkly 133:563–566

    PubMed  CAS  Google Scholar 

  16. Yumru M, Savas HA, Kalenderoglu A, Bulut M, Celik H, Erel O (2009) Oxidative imbalance in bipolar disorder subtypes: a comparative study. Prog Neuropsychopharmacol Biol Psychiatry 33:1070–1074

    Article  PubMed  CAS  Google Scholar 

  17. Kosecik M, Erel O, Sevinc E, Selek S (2005) Increased oxidative stress in children exposed to passive smoking. Int J Cardiol 100:61–64

    Article  PubMed  Google Scholar 

  18. Aktan B, Taysi S, Gumustekin K, Uçüncü H, Memişoğullari R, Save K, Bakan N (2003) Effect of macrolide antibiotics on nitric oxide synthase and xanthine oxidase activities, and malondialdehyde level in erythrocyte of the guinea pigs with experimental otitis media with effusion. Pol J Pharmacol 55:1105–1110

    PubMed  CAS  Google Scholar 

  19. Ece A, Atamer Y, Gürkan F, Davutoğlu M, Bilici M, Tutanç M (2006) Paraoxonase, anti-oxidant response and oxidative stress in children with chronic renal failure. Pediatr Nephrol 21:239–245

    Article  PubMed  Google Scholar 

  20. Yılmaz T, Koçan EG, Besler HT (2004) The role of antioxidants in chronic tonsillitis and adenoid hypertrophy in children. Int J Pediatr Otorhinolaryngol 68:1053–1058

    Article  PubMed  Google Scholar 

  21. Schulpis KH, Barzelitou M, Papadakis M, Rodolakis A, Antsaklis A, Papassotiriou I, Vlachos GD (2008) Maternal chronic hepatitis B virus is implicated with low neonatal paraoxonase/arylesterase activities. Clin Biochem 41:282–287

    Article  PubMed  CAS  Google Scholar 

  22. Selek S, Coşar N, Koçyiğit A, Erel O, Aksoy N, Gencer M, Gunak F, Aslan M (2008) PON1 activity and total oxidant status in patients with active pulmonary tuberculosis. Clin Biochem 41:140–144

    Article  PubMed  CAS  Google Scholar 

  23. Çakmak A, Zeyrek D, Atas A, Selek S, Erel O (2009) Oxidative status and paraoxonase activity in children with asthma. Clin Invest Med 32:327–334

    Google Scholar 

  24. Shih DM, Lusis AJ (2009) The roles of PON1 and PON2 in cardiovascular disease and innate immunity. Curr Opin Lipidol 20:288–292

    Article  PubMed  CAS  Google Scholar 

  25. Tanimoto N, Kumon Y, Suehiro T, Ohkubo S, Ikeda Y, Nishiya K, Hashimoto K (2003) Serum paraoxonase activity decreases in rheumatoid arthritis. Life Sci 72:2877–2885

    Article  PubMed  CAS  Google Scholar 

  26. Kiss E, Seres I, Tarr T, Kocsis Z, Szegedi G, Paragh G (2007) Reduced paraoxonase1 activity is a risk for atherosclerosis in patients with systemic lupus erythematosus. Ann NY Acad Sci 1108:83–91

    Article  PubMed  CAS  Google Scholar 

  27. Toker A, Kadi M, Yildirim AK, Aksoy H, Akcay F (2009) Serum lipid profile paraoxonase and arylesterase activities in psoriasis. Cell Biochem Funct 27:176–180

    Article  PubMed  CAS  Google Scholar 

  28. Karakucuk S, Baskol G, Oner AO, Baskol M, Mirza E, Ustdal M (2004) Serum paraoxonase activity is decreased in the active stage of behcet’s disease. Br J Ophthalmol 88:1256–1258

    Article  PubMed  CAS  Google Scholar 

  29. Shigemi H, Egashira T, Kurono Y, Mogi G (1998) Role of superoxide dismutase in otitis media with effusion. Ann Otol Rhinol Laryngol 107:327–331

    PubMed  CAS  Google Scholar 

  30. Yariktas M, Doner F, Dogru H, Yasan H, Delibas N (2004) The role of free oxygen radicals on the development of otitis media with effusion. Int J Pediatr Otorhinolaryngol 68:889–894

    Article  PubMed  Google Scholar 

  31. Hamzei M, Ventriglia G, Hagnia M, Antonopolous A, Bernal-Sprekelsen M, Dazert S, Hildmann H, Sudhoff H (2003) Osteoclast stimulating and differentiating factors in human cholesteatoma. Laryngoscope 113:436–442

    Article  PubMed  CAS  Google Scholar 

  32. Chole RA, Faddis BT (2002) Evidence for microbial biofilms in cholesteatomas. Arch Otolaryngol Head Neck Surg 128:1129–1133

    PubMed  Google Scholar 

  33. Jung JY, Pashia ME, Nishimoto SY, Faddis BT, Chole RA (2004) A possible role for nitric oxide in osteoclastogenesis associated with cholesteatoma. Otol Neurotol 25:661–668

    Article  PubMed  Google Scholar 

  34. Camps J, Pujol I, Ballester F, Joven J, Simó JM (2011) Paraoxonases as potential antibiofilm agents: their relationship with quorum-sensing signals in gram-negative bacteria. Antimicrob Agents Chemother 55:1325–1331

    Article  PubMed  CAS  Google Scholar 

  35. Cakmak A, Zeyrek D, Atas A, Celik H, Aksoy N, Erel O (2009) Serum prolidase activity and oxidative status in patients with bronchial asthma. J Clin Lab Anal 23:132–138

    Article  PubMed  CAS  Google Scholar 

  36. Powell GF, Kurosky A, Maniscalco RM (1977) Prolidase deficiency: report of a second case with quantitation of the excessively excreted amino acids. J Pediatr 91:242–246

    Google Scholar 

  37. Amar MS, Wishahi HF, Zakhary MM (1996) Clinical and biochemical studies of bone destruction in cholesteatoma. J Laryngol Otol 110:534–539

    Article  PubMed  CAS  Google Scholar 

  38. Aberg B, Bagger-Sjöbäck D, Edström S, Heyden G, Jacobsson M, Kindblom LG (1990) A comparative study of enzyme histochemical features in the gerbilline and human cholesteatoma. Acta Otolaryngol 109:130–146

    Article  PubMed  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Otolaryngology, Medical Faculty, Gaziantep University, Sahinbey, Gaziantep, 27070, Turkey

    Elif Baysal, Fatih Celenk & Muzaffer Kanlıkama

  2. Department of Biochemistry, Medical Faculty, Harran University, Sanliurfa, Turkey

    Nurten Aksoy, Abdullah Taşkın & Hasan Bilinç

  3. Department of Otolaryngology, Kahta State Hospital, Adiyaman, Turkey

    Ferit Kara

  4. Department of Biochemistry, Medical Faculty, Gaziantep University, Gaziantep, Turkey

    Seyithan Taysi

  5. Department of Otolaryngology, Medical Faculty, Mustafa Kemal University, Hatay, Turkey

    Cengiz Cevik

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  1. Elif Baysal
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  2. Nurten Aksoy
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  5. Abdullah Taşkın
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Correspondence to Elif Baysal.

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Baysal, E., Aksoy, N., Kara, F. et al. Oxidative stress in chronic otitis media. Eur Arch Otorhinolaryngol 270, 1203–1208 (2013). https://doi.org/10.1007/s00405-012-2070-z

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  • DOI: https://doi.org/10.1007/s00405-012-2070-z

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