Abstract
Introduction
This animal model was designed to evaluate the effect of intra-articular iron-chelator treatment in the prevention of blood-induced joint disease.
Method
Thirty domestic male rabbits were divided into three equal groups. One milliliter of homologous blood was injected into the left knee of each rabbit in groups 1 and 2. In the second group, 0.1 ml desferroxamine mesilate (DM) was also injected to the blood. In group 3, the animals received injections of 0.1 DM which was mixed with equal amounts of sterile saline solution. The joints were injected three times each week for 12 weeks. At the end of 12 weeks, the knee joints of each rabbit were examined.
Results
The synovium in group 1 was found statistically significantly thicker than in group 2. Obvious erosion and ulceration of the cartilage were seen in all joints in group 1 and absent in group 2. Iron-chelator treatment reduced the formation of blood-induced joint damage in rabbit knees by inhibiting the iron-catalyzed formation of destructive oxygen metabolites that has an effect on joint cartilage and synovium.
Similar content being viewed by others
References
Hooiveld MJ, Roosendaal G, van den Berg HM, Bijlsma JW, Lafeber FP (2003) Haemoglobin-derived iron-dependent hydroxyl radical formation in blood-induced joint damage: an in vitro study. Rheumatology 42(6):784–790 Epub 31 Mar 2003
Hooiveld MJ, Roosendaal G, Jacobs KM, Vianen ME, van den Berg HM, Bijlsma JW, Lafeber FP (2004) Initiation of degenerative joint damage by experimental bleeding combined with loading of the joint: a possible mechanism of hemophilic arthropathy. Arthritis Rheum 50(6):2024–2031
Hooiveld M, Roosendaal G, Vianen M, van den Berg M, Bijlsma J, Lafeber F (2003) Blood-induced joint damage: longterm effects in vitro and in vivo. J Rheumatol 30(2):339–344
Madhok R, Bennett D, Sturrock RD, Forbes CD (1988) Mechanisms of joint damage in an experimental model of hemophilic arthritis. Arthritis Rheum 31(9):1148–1155
Colombo C, Butler M, O’Byrne E, Hickman L, Swartzendruber D, Selwyn M, Steinetz B (1983) A new model of osteoarthritis in rabbits. I. Development of knee joint pathology following lateral meniscectomy and section of the fibular collateral and sesamoid ligaments. Arthritis Rheum 26(7):875–886
Ribbans WJ, Giangrande P, Beeton K (1997) Conservative treatment of hemarthrosis for prevention of hemophilic synovitis. Clin Orthop Relat Res (343):12–18
Britton RS, Leicester KL, Bacon BR (2002) Iron toxicity and chelation therapy. Int J Hematol 76(3):219–228
Brittenham GM, Griffith PM, Nienhuis AW, McLaren CE, Young NS, Tucker EE, Allen CJ, Farrell DE, Harris JW (1994) Efficacy of deferoxamine in preventing complications of iron overload in patients with thalassemia major. N Engl J Med 331(9):567–573
Luchtman-Jones L, Valentino LA, Manno C (2006) Recombinant therapy workshop participants. Considerations in the evaluation of haemophilia patients for short-term prophylactic therapy: a paediatric and adult case study. Haemophilia 12(1):82–86
Valentino LA, Hakobyan N, Kazarian T, Jabbar KJ, Jabbar AA (2004) Experimental haemophilic synovitis: rationale and development of a murine model of human factor VIII deficiency. Haemophilia 10(3):280–287
Fabry G (1990) Ultrastructural changes in synovium and cartilage in experimental hemarthrosis in dogs. Arch Orthop Trauma Surg 109(1):21–29
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kocaoğlu, B., Akgun, U., Erol, B. et al. Preventing blood-induced joint damage with the use of intra-articular iron chelators: an experimental study in rabbits. Arch Orthop Trauma Surg 129, 1571–1575 (2009). https://doi.org/10.1007/s00402-009-0885-x
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00402-009-0885-x