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Increased expression of podoplanin in malignant astrocytic tumors as a novel molecular marker of malignant progression

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Abstract

Podoplanin (aggrus) is a mucin-like transmembrane sialoglycoprotein that is expressed on lymphatic endothelial cells. Podoplanin is putatively involved in cancer cell migration, invasion, metastasis, and malignant progression and may be involved in platelet aggregation. Previously, we showed upregulated expression of podoplanin in central nervous system (CNS) germinomas, but not in non-germinomatous germ cell tumors, except for parts of immature teratomas in limited numbers. However, little information exists about its role in CNS astrocytic tumors. In this study, 188 astrocytic tumors (30 diffuse astrocytomas, 43 anaplastic astrocytomas, and 115 glioblastomas) were investigated using immunohistochemistry with an anti-podoplanin antibody, YM-1. In 11 of 43 anaplastic astrocytomas (25.6%) and in 54 of 115 glioblastomas (47.0%), podoplanin was expressed on the surface of anaplastic astrocytoma cells and glioblastoma cells, especially around necrotic areas and proliferating endothelial cells. However, the surrounding brain parenchyma was not stained by YM-1. On the other hand, podoplanin expression was not observed in diffuse astrocytoma (0/30: 0%). Furthermore, we investigated the expression of podoplanin using quantitative real-time PCR and Western blot analysis in 54 frozen astrocytic tumors (6 diffuse astrocytomas, 14 anaplastic astrocytomas, and 34 glioblastomas). Podoplanin mRNA and protein expression were markedly higher in glioblastomas than in anaplastic astrocytomas. These data suggest that podoplanin expression might be associated with malignancy of astrocytic tumors.

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Acknowledgments

This study was supported in part by Kanae Foundation for Life and Socio-medical Science (to Y.K.) and by Osaka Cancer Research Foundation (to Y.K.). We thank Drs Fujita and Tsuruo (University of Tokyo) for their great help, and Ms Kunita, Mr Nakazawa (University of Tokyo), Ms Totake, and Ms Kobo (Saitama Medical School) for their kind assistance. We thank Dr Sugiyama (Hiroshima University) for providing us clinical samples.

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Authors and Affiliations

  1. Department of Neurosurgery, Saitama Medical School, 38 Morohongo Moroyama-machi Iruma-gun, Saitama, 350-0495, Japan

    Kazuhiko Mishima, Ryo Nishikawa & Masao Matsutani

  2. Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Open Space Laboratory C-2, 1-1-1 Umezono, Tsukuba, Ibaraki, 305-8568, Japan

    Yukinari Kato & Mika Kato Kaneko

  3. Department of Pathology, Saitama Medical School, 38 Morohongo Moroyama-machi Iruma-gun, Saitama, 350-0495, Japan

    Takanori Hirose

Authors
  1. Kazuhiko Mishima
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  2. Yukinari Kato
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  3. Mika Kato Kaneko
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  4. Ryo Nishikawa
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  5. Takanori Hirose
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  6. Masao Matsutani
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Correspondence to Yukinari Kato.

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Mishima, K., Kato, Y., Kaneko, M.K. et al. Increased expression of podoplanin in malignant astrocytic tumors as a novel molecular marker of malignant progression. Acta Neuropathol 111, 483–488 (2006). https://doi.org/10.1007/s00401-006-0063-y

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  • DOI: https://doi.org/10.1007/s00401-006-0063-y

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