Abstract
A microvillus-like structure observed in digestive systems and glands was fabricated by the photopolymerization-induced self-assembly using an amphiphilic random block copolymer consisting of hydrophilic poly(methacrylic acid), PMAA, and hydrophobic poly(methyl methacrylate-random-methacrylic acid), P(MMA-r-MAA). The random block copolymerization of methyl methacrylate and methacrylic acid using a PMAA end-capped with 4-methoxy-2,2,6,6-tetramethylpiperidine-1-oxyl for the photopolymerization-induced self-assembly produced spherical vesicles and micelles during the early stage of the polymerization. These spherical particles transformed into worm-like vesicles, accompanied by the precipitation of a film-like product as the hydrophobic block chain grew according to the polymerization progress. Further extension of the hydrophobic block length produced the microvillus-like structure on the surface of the precipitating film. The finger-like structure in the microvilli had an ca. 8.5-μm height. An inverted cone-like critical packing shape of the copolymer with the hydrophobic block longer than the hydrophilic block contributed to the formation of the microvillus-like structure.
References
Getz GS, Bartley W, Lurie D, Notton BM (1968) Biochim Biophys Acta 152:325
Verkleij AJ, Zwaal RFA, Roelofsen B, Comfurius P, Kastelijn D, Van Deenen LLM (1973) Biochim Biophys Acta 323:178
Tsai KH, Lenard J (1975) Nature 253:554
Rothman JE, Tsai DK, Dawidowicz EA, Lenard J (1976) Biochemistry 15:2361
Israelachvili JN (2011) Intermolecular and surface forces, 3rd edn. Academic, Waltham, p 559
Anderson RGW, Brown MS, Goldstein JL (1977) Cell 10:351
Norris V, Misevic G, Delosme J, Oshima A (2002) J Mol Biol 318:455
Papanikolaou A, Papafotika A, Murphy C, Papamarcaki T, Tsolas O, Drab M, Kurzchalia TV, Kasper M, Christoforidis S (2005) J Biol Chem 280:26406
Kessel RG, Kardon RH (1979) Tissues and organs, a text-atlas of scanning electron microscopy. W. H. Freeman and Company, San Francisco, p 199
Kawai K, Fujita M, Nakao M (1974) Biochim Biophys Acta 369:222
Yoshida E (2013) Colloid Polym Sci 291:2733
Yoshida E (2014) Colloid Polym Sci 292:1463
Yoshida E (2014) Colloid Polym Sci 292:763
Yoshida E (2014) Colloid Polym Sci 292:2555
Yoshida E (2015) Colloid Polym Sci 293:249
Yoshida E (2015) Supramol Chem 27:274
Yoshida E (2015) Colloid Polym Sci 293:649
Yoshida E (2015) Colloid Polym Sci 293:1275
Yoshida E (2012) International Scholarly Research Network (ISRN) Polym Sci, Article 102186. doi:10.5402/2012/102186
Kobayashi S, Uyama H, Yamamoto I, Matsumoto Y (1990) Polym J 22:759
Yoshida E (2009) Colloid Polym Sci 287:767
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The author is thankful for a JSPS Grant-in-Aid for Scientific Research (Grant Number 25390003).
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Yoshida, E. Fabrication of microvillus-like structure by photopolymerization-induced self-assembly of an amphiphilic random block copolymer. Colloid Polym Sci 293, 1841–1845 (2015). https://doi.org/10.1007/s00396-015-3600-1
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DOI: https://doi.org/10.1007/s00396-015-3600-1