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Catharanthus roseus leaf extract-synthesized chitosan nanoparticles for controlled in vitro release of chloramphenicol and ketoconazole

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Abstract

We report the size-controlled chitosan nanoparticles synthesized by Catharanthus roseus leaf extract-mediated cross-linking of chitosan. Optimization of pH, ratio of chitosan to leaf filtrate concentration, and cross-linking time was performed for designing factorial synthetic strategy, which revealed minimum diameter (45 nm) of chitosan nanoparticles at pH 3 by employing chitosan/leaf extract in the ratio 3:1. The biogenically synthesized chitosan nanoparticles were further explored for encapsulation and controlled release of ketoconazole and chloramphenicol. The resulting drug-loaded chitosan nanoparticles showed size-dependent drug entrapment efficiency of 82.88 and 78.96 % with drug-loading capacity of 41.44 and 39.48 % for chloramphenicol and ketoconazole, respectively. The cumulative in vitro drug release up to 12 h was achieved which suggests enhanced efficacy of green synthesized chitosan nanoparticles for therapeutic and drug delivery applications. So far, there is no report concerning biogenic synthesis of chitosan nanoparticles; hence, it is being reported for the first time.

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Acknowledgments

The authors acknowledge financial support from Department of Science and Technology for providing INSPIRE fellowship and project under “Nano mission Programme” and UGC, New Delhi for financial assistance under SAP program.

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Authors and Affiliations

  1. Nanobiotechnology Laboratory, Department of Biotechnology, SGB Amravati University, Amravati, 444602, Maharashtra, India

    Dipali Nagaonkar, Swapnil Gaikwad & Mahendra Rai

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  1. Dipali Nagaonkar
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  2. Swapnil Gaikwad
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  3. Mahendra Rai
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Correspondence to Mahendra Rai.

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Nagaonkar, D., Gaikwad, S. & Rai, M. Catharanthus roseus leaf extract-synthesized chitosan nanoparticles for controlled in vitro release of chloramphenicol and ketoconazole. Colloid Polym Sci 293, 1465–1473 (2015). https://doi.org/10.1007/s00396-015-3538-3

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  • DOI: https://doi.org/10.1007/s00396-015-3538-3

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