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Influence of trans fatty acids on glucose metabolism in soleus muscle of rats fed diets enriched in or deprived of linoleic acid

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Abstract

Purpose

Industrial trans fatty acid (TFA) intake leads to impaired glucose metabolism. However, the overall effects reported are inconsistent and vary with the dietary FA composition and TFA isomer type and levels. We investigated TFA effects on glucose uptake, incorporation and oxidation, and glycogen synthesis in incubated soleus muscle under basal conditions or after treatment with insulin and/or palmitate.

Methods

Male Wistar rats were fed either linoleic acid (LA)-enriched (+LA) or LA-deprived (−LA) diet, supplemented (+LA + TFA or −LA + TFA) or not with TFA, for 60 days. Soleus muscle glucose metabolism was assessed in the absence or presence of insulin and/or palmitic acid.

Results

Under basal conditions, TFA enhanced glucose uptake and oxidation regardless of the LA status. Both TFA-supplemented groups had lower insulin response to glucose metabolism. Under insulin-stimulated conditions, TFA prevented the palmitate inhibition of muscle glucose uptake and metabolism in the +LA + TFA group.

Conclusion

Dietary TFA enhanced glucose utilization in incubated soleus muscle under basal conditions and prevented the palmitate-induced inhibition in insulin-stimulated conditions. However, TFA reduced the insulin response to glucose uptake and metabolism. The effects mentioned above were influenced by the FA profile modifications induced by the dietary LA levels, suggesting that lipid metabolization and incorporation into plasma membrane are important determining factors of glucose metabolism and insulin sensitivity.

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Acknowledgements

We wish to thank Walter DaRú for his technical assistance. We also thank Universidad Nacional del Litoral-Cursos de Acción para la Investigación y Desarrollo (CAI + D 501 201101 00165 LI)-Secretaría de Ciencia y Técnica-UNL, Consejo Nacional de Investigaciones Científicas y Técnicas (PIP CONICET No. 112-200801-02831), FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) and CONICET Resol. No. 473/11, for their financial support to the collaborative research program between the Universidad Nacional del Litoral and the Universidade de São Paulo.

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Authors and Affiliations

  1. Cátedra Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, C.C. 242, 3000, Santa Fe, Argentina

    Ana C. Fariña, Juliana Sain, Marcela González & Claudio Bernal

  2. Institute of Physical Activity Sciences and Sports, Cruzeiro do Sul University, Sao Paulo, Brazil

    Sandro Hirabara

  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santa Fe, Argentina

    Juliana Sain & Claudio Bernal

  4. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

    Rui Curi

  5. Post-Graduate Program in Human Health Sciences, Biological Sciences and Health Center, Cruzeiro do Sul University, Sao Paulo, Brazil

    Rui Curi

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  1. Ana C. Fariña
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  2. Sandro Hirabara
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  3. Juliana Sain
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  4. Marcela González
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  5. Rui Curi
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  6. Claudio Bernal
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Correspondence to Claudio Bernal.

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There are no conflicts of interest in this study.

Ethical approval

The experimental procedures were approved by the Ethics Committee of the School of Biochemistry and by the Ethics Committee of the Institute of Biomedical Sciences, University of Sao Paulo. Both followed the Guide for the Care and Use of Laboratory Animals.

Additional information

Ana C. Fariña and Sandro Hirabara contributed equally to this work.

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Fariña, A.C., Hirabara, S., Sain, J. et al. Influence of trans fatty acids on glucose metabolism in soleus muscle of rats fed diets enriched in or deprived of linoleic acid. Eur J Nutr 57, 1343–1355 (2018). https://doi.org/10.1007/s00394-017-1413-3

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  • DOI: https://doi.org/10.1007/s00394-017-1413-3

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