Abstract
Purpose
In this work, the absorption and/or bioavailability of iron from two chemical species, 57Fe-Lf (apo-lactoferrin) complex and 57FeSO4 at low and high dose, and in Lf excess were investigated in lactating wistar rats.
Methods
The methodology used is based on the use of stable isotopes in combination with the approach “isotope pattern deconvolution” and ICP-MS for detection. This approach provides quantitative information about exogenous (57Fe) and endogenous iron (natFe) distribution in fluids and tissues in the iron-supplemented rat groups.
Results
The observed results with supplemented rats were compared with those found in rats receiving maternal feeding. Interestingly, differences were found between groups in iron for transport and storage compartments, but not in the functional one, depending upon the dose of iron administered and the chemical species.
Conclusion
Considering the results obtained, supplementation with iron salts in excess of Lf appears to be the best way of iron supplementation of formula milk.
Similar content being viewed by others
References
McLean E, Cogswell M, Egli I, Wojdyla D, de Benoist B (2009) Worldwide prevalence of anaemia, WHO vitamin and mineral nutrition information system, 1993–2005. Public Health Nutr 12(4):444–454. doi:10.1017/S1368980008002401
Gisbert JP, Gomollón F (2009) An update on iron physiology. World J Gastroenterol 15:4617–4626
Mertz W, Underwood EJ (1986) Trace elements in human and animal nutrition. Academic Press Inc., Nueva York (EEUU). ISBN 0-1249-1252-4
Moy RJD (2000) Iron fortification of infant formula. Nutr Res Rev 13:215–227. doi:10.1079/095442200108729070
Brätter P, Blasco IN, Negretti de Brätter VE, Raab A (1998) Speciation as an analytical aid in trace element research in infant nutrition. Analyst 123:821–826
Fernández-Sánchez ML, de la Flor St. Remy R, González-Iglesias H, López-Sastre JB, Fernández-Colomer B, Pérez-Solís D, Sanz-Medel A (2012) Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: a comparison with commercial infant milk formulas. Microchem J 105:108–114. doi:10.1016/j.microc.2012.03.016
Davidsson L, Kastenmayer P, Yuen M, Lonnerdal B, Huirrell RF (1994) Influence of lactoferrin on iron absorption from human milk in infants. Pediatr Res 35:117–124
Brock JH (1980) Lactoferrin in human milk: its role in iron absorption and protection against enteric infection in the newborn infant. Arch Dis Child 55:417–421
Lönnerdal B, Bryant A (2006) Absorption of iron from recombinant human lactoferrin in young US women. Am J Clin Nutr 83:305–309
García-Alonso JI, Rodriguez-González P (2013) Isotope dilution mass spectrometry. The Royal Society of Chemistry Publishing, London. ISBN 978-1-84973-333-5
González-Iglesias H, Fernández-Sánchez ML, López-Sastre JB, Sanz-Medel A (2012) Nutritional iron supplementation studies based on enriched 57Fe, added to milk in rats, and isotope pattern deconvolution-ICP-MS analysis. Electrophoresis 33:2407–2415. doi:10.1002/elps.201100334
Feng M, Van der Does L, Bantjes A (1995) Preparation of apolactoferrin with a very low iron saturation. J Dairy Sci 78:2352–2357
Messerschmidt A, Huber R, Poulos T, Wieghardt K (2011) Handbook of metalloproteins, vol 2. Wiley, New York. ISBN 9780000000298
Yamauchi K, Toida T, Nishimura S, Nagano E, Kusuoka O, Teraguchi S, Hayasawa H, Shimamura S, Tomita T (2000) 13-week oral repeated administration toxicity study of bovine lactoferrin in rats. Food Chem Toxicol 38:503–512
Codex Stand, Standard for Infant Formula and Formulas for Special Medical Purposes Intended for Infants (1981; Amendment 2011) Ref. 72-1981. Food and Agriculture Organization of the United Nations, World Health Organization. http://www.codexalimentarius.org/standards/list-of-standards/en/?provide=standards&orderField=fullReference&sort=asc&num1=CODEX (visited on 14th December 2015)
Frazer DM, Wilkins SJ, Anderson GJ (2007) Elevated iron absorption in the neonatal rat reflects high expression of iron transport genes in the distal alimentary tract. Am J Physiol Gastrointest Liver Physiol 293:525–531
Domellöf M, Lönnerdal B, Abrams SA, Hernell O (2002) Iron absorption in breast-fed infants: effects of age, iron status, iron supplements, and complementary foods. Am J Clin Nutr 76:198–204
Lee HB, Balaufox MD (1985) Blood volume in the rat. J Nucl Med 26:72–76
Scott BJ, Bradwell AR (1983) Identification of the serum binding proteins for iron, zinc, cadmium, nickel, and calcium. Clin Chem 29:629–633
Gürsel FE, Ates A, Bilal T, Altiner A (2012) Effect of dietary garcinia cambogia extract on serum essential minerals (calcium, phosphorus, magnesium and trace elements (iron, copper, zinc) in rats fed with high-lipid diet. Biol Trace Elem Res 148:378–382. doi:10.1007/s12011-012-9385-x
Crichton RR, Charloteaux-Wauters MC (1987) Iron transport and storage. Eur J Biochem 164:485–506
Takahashi S, Takahashi I, Sato H, Kubota Y, Yoshida S, Muramatsu Y (2000) Determination of major and trace elements in the liver of Wistar rats by inductively coupled plasma-atomic emission spectrometry and mass spectrometry. Lab Anim 34:97–105
Sherman AR, Tissue NT (1981) Tissue iron, copper and zinc levels in offspring of iron-sufficient and iron-deficient rats. J Nutr 111:266–275
Konz T, Montes-Bayón M, Bettmer J, Sanz-Medel A (2011) Analysis of hepcidin, a key peptide for Fe homeostasis, via sulfur detection by capillary liquid chromatography-inductively coupled plasma mass spectrometry. J Anal At Spectrom 26:334–340. doi:10.1039/C0JA00053A
Liu Y, Templeton DM (2015) Iron-dependent turnover of IRP-1/c-acotinase in kidney cells. Metallomics 7:766–775
Acknowledgments
M. L. F. S. and A. S. M. designed research; S. F. M. conducted research; B. F. C., J. L. S, H. G. I. and S. F. M. analysed data; S. F. M., M. L. F. S. and A. S. M. wrote the paper. All authors have read and approved the final manuscript. On behalf of all authors, the corresponding author states that there is no conflict of interest. Authors are grateful to “Fundación para la Investigación Científica Aplicada y la Tecnología, Principado de Asturias” (FICYT. FC-11-PC10-46) and to “Fondo Europeo de Desarrollo Regional” (FEDER). Also financial support from “Laboratorios Ordesa” (Barcelona, Spain) and “Fundación Grupo Castrillo” (Spain) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding authors
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Fernández-Menéndez, S., Fernández-Sánchez, M.L., González-Iglesias, H. et al. Iron bioavailability from supplemented formula milk: effect of lactoferrin addition. Eur J Nutr 56, 2611–2620 (2017). https://doi.org/10.1007/s00394-016-1325-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00394-016-1325-7