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Food derived carbonyl compounds affect basal and stimulated secretion of interleukin-6 and -8 in Caco-2 cells

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Abstract

Background

The carbonyl compounds methylglyoxal (MG) and glyoxal (G) are reactive intermediates generated in a variety of foods and beverages during processing and prolonged storage.

Aim and methods

We investigated direct effects of these compounds on intestinal cells determining the basal and stimulated secretion of IL-8 and IL-6 in vitro.

Results

MG or G induced a concentration dependent enhancement of IL-8 and IL-6 secretion compared to baseline levels. A co-incubation with pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) or lipopolysaccharides (LPS) and increasing MG concentrations further enhanced IL-8 and IL-6 secretion. For G, however, this additive effect was only observed in TNF-α and IL-1β treated cells, but not after co-incubation with LPS.

Conclusion

These results suggest a pro-inflammatory effect of G and MG at high concentrations in human intestinal cells by stimulating IL-8 and IL-6 cytokine levels. Effects of G and MG in combination with other cytokines may negatively affect inflammatory processes.

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References

  1. Akhand AA, Hossain K, Mitsui H, Kato M, Miyata T, Inagi R, Du J, Takeda K, Kawamoto Y, Suzuki H, Kurokawa K, Nakashima I (2001) Glyoxal and methylglyoxal trigger distinct signals for map family kinases and caspase activation in human endothelial cells. Free Radic Biol Med 31:20–30

    Article  CAS  Google Scholar 

  2. Baskaran S, Balasubramanian KA (1990) Toxicity of methylglyoxal towards rat enterocytes and colonocytes. Biochem Int 21:165–174

    CAS  Google Scholar 

  3. Baskaran S, Balasubramanian KA (1990) Effect of methylglyoxal on protein thiol and amino groups in isolated rat enterocytes and colonocytes and activity of various brush border enzymes. Indian J Biochem Biophys 27:13–17

    CAS  Google Scholar 

  4. Cantero AV, Portero-Otín M, Ayala V, Auge N, Sanson M, Elbaz M, Thiers JC, Pamplona R, Salvayre R, Nègre-Salvayre A (2007) Methylglyoxal induces advanced glycation end product (AGEs) formation and dysfunction of PDGF receptor-{beta}: implications for diabetic atherosclerosis. FASEB J 21:3096–3106

    Article  CAS  Google Scholar 

  5. Daglia M, Papetti A, Grisoli P, Aceti C, Spini V, Dacarro C, Gazzani G (2007) Isolation, identification, and quantification of roasted coffee antibacterial compounds. Agric Food Chem 55:10208–10213

    Article  CAS  Google Scholar 

  6. de Revel G, Bertrand A (1994) Trends in flavour research. In: Maarse H, van der Heij DG (eds) Proceedings of the 7th Weurman flavour research symposium, Elsevier, Zeist, pp 353–361

  7. de Revel G, Martin N, Pripis-Nicolau L, Lonvaud-Funel A, Bertrand A (1999) Contribution to the knowledge of malolactic fermentation influence on wine aroma. J Agric Food Chem 47:4003–4008

    Article  Google Scholar 

  8. Fujioka K, Shibamoto T (2004) Formation of genotoxic dicarbonyl compounds in dietary oils upon oxidation. Lipids 39:481–486

    Article  CAS  Google Scholar 

  9. Hoffmann E, Dittrich-Breiholz O, Holtmann H, Kracht M (2002) Multiple control of interleukin-8 gene expression. J Leukoc Biol. 72:847–855

    CAS  Google Scholar 

  10. Kuntz S, Rudloff S, Kunz C (2007) Oligosaccharides from human milk influence growth-related characteristics of intestinally transformed and non-transformed intestinal cells. Br J Nutr 99:462–471

    Google Scholar 

  11. Lapolla A, Flamini R, Dalla Vedova A, Senesi A, Reitano R, Fedele D, Basso E, Seraglia R, Traldi P (2003) Glyoxal and methylglyoxal levels in diabetic patients: quantitative determination by a new GC/MS method. Clin Chem Lab Med 41:1166–1173

    Article  CAS  Google Scholar 

  12. Liu BF, Miyata S, Hirota Y, Higo S, Miyazaki H, Fukunaga M, Hamada Y, Ueyama S, Muramoto O, Uriuhara A, Kasuga M (2003) Methylglyoxal induces apoptosis through activation of p38 mitogen-activated protein kinase in rat mesangial cells. Kidney Int 63:947–957

    Article  CAS  Google Scholar 

  13. McLellan AC, Thornalley PJ, Benn J, Sonksen PH (1994) Glyoxalase system in clinical diabetes mellitus and correlation with diabetic complications. Clin Sci 87:21–29

    CAS  Google Scholar 

  14. Migliore L, Barale R, Bosco E, Giorgelli F, Minunni M, Scarpato R, Loprieno N (1990) Genotoxicity of methylglyoxal: cytogenetic damage in human lymphocytes in vitro and in intestinal cells of mice. Carcinogenesis 11:1503–1507

    Article  CAS  Google Scholar 

  15. Miller AG, Gerrard JA (2005) Assessment of protein function following cross-linking by alpha-dicarbonyls. Ann NY Acad Sci 1043:195–200

    Article  CAS  Google Scholar 

  16. Ndengele MM, Muscoli C, Wang ZQ, Doyle TM, Matuschak GM, Salvemini D (2005) Superoxide potentiates NF-kappaB activation and modulates endotoxin-induced cytokine production in alveolar macrophages. Shock 23:186–193

    Article  CAS  Google Scholar 

  17. Nagao M, Fujita Y, Wakabayashi K, Nukaya H, Kosuge T, Sugimura T (1986) Mutagens in coffee and other beverages. Environ Health Perspect 67:89–91

    Article  CAS  Google Scholar 

  18. Nemet I, Varga-Defterdarović L, Turk Z (2006) Methylglyoxal in food and living organisms. Mol Nutr Food Res 50:1105–1117

    Article  CAS  Google Scholar 

  19. Niyati-Shirkhodaee F, Shibamoto T (1986) Gas chromatographic analysis of glyoxal and methylglyoxal formed from lipids and related compounds upon ultraviolet irridation. J Agric Food Chem 41:227–230

    Article  Google Scholar 

  20. Pizarro TT, De La Rue SA, Cominelli F (2006) Role of interleukin 6 in a murine model of Crohn’s ileitis: are cytokine/anticytokine strategies the future for IBD therapies? Gut 55:1226–1227

    Article  CAS  Google Scholar 

  21. Randell EW, Vasdev S, Gill V (2005) Measurement of methylglyoxal in rat tissues by electrospray ionization mass spectrometry and liquid chromatography. J Pharmacol Toxicol Methods 51:153–157

    Article  CAS  Google Scholar 

  22. Roebuck KA (1999) Regulation of interleukin-8 gene expression. J Interferon Cytokine Res 19:429–438

    Article  CAS  Google Scholar 

  23. Shangari N, Depeint F, Furrer R, Bruce WR, Popovic M, Zheng F, O’Brien PJ (2007) A thermolyzed diet increases oxidative stress, plasma alpha-aldehydes and colonic inflammation in the rat. Chem Biol Interact 169:100–109

    Article  CAS  Google Scholar 

  24. Tan D, Wang Y, Lo CY, Sang S, Ho CT (2008) Methylglyoxal: its presence in beverages and potential scavengers. Ann NY Acad Sci 1126:72–75

    Article  CAS  Google Scholar 

  25. Uribarri J, Cai W, Peppa M, Goodman S, Ferrucci L, Striker G, Vlassara H (2007) Circulating glycotoxins and dietary advanced glycation endproducts: two links to inflammatory response, oxidative stress, and aging. J Gerontol A Biol Sci Med Sci 62:427–433

    Google Scholar 

  26. Wang H, Meng QH, Gordon JR, Khandwala H, Wu L (2007) Proinflammatory and proapoptotic effects of methylglyoxal on neutrophils from patients with type 2 diabetes mellitus. Clin Biochem 40:1232–1239

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Nadine Metz for her excellent technical assistance. This work was supported by the Hessian Ministry of Higher Education, Research, and the Arts (HMWK).

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Authors and Affiliations

  1. Institute of Nutritional Science, University of Giessen, Wilhelmstrasse 20, 35392, Giessen, Germany

    Sabine Kuntz, Julia Ehl & Clemens Kunz

  2. Department of Pediatrics, University of Giessen, Feulgenstrasse 12, 35392, Giessen, Germany

    Silvia Rudloff

  3. Medical Clinic III and Policlinic, University of Giessen, Rodthohl 6, 35392, Giessen, Germany

    Reinhard G. Bretzel

Authors
  1. Sabine Kuntz
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  2. Silvia Rudloff
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  3. Julia Ehl
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  4. Reinhard G. Bretzel
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  5. Clemens Kunz
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Correspondence to Sabine Kuntz.

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Kuntz, S., Rudloff, S., Ehl, J. et al. Food derived carbonyl compounds affect basal and stimulated secretion of interleukin-6 and -8 in Caco-2 cells. Eur J Nutr 48, 499–503 (2009). https://doi.org/10.1007/s00394-009-0035-9

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  • DOI: https://doi.org/10.1007/s00394-009-0035-9

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