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Gene expression profiles in the liver of mice irradiated with 60Co gamma rays and treated with soybean isoflavone

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Abstract

Purpose

To better understand the molecular mechanisms underlying the radio-protective effect of soybean isoflavone that we observed in our recent animal experiments.

Materials and methods

We utilized a cDNA microarray to investigate the expression profiles of 4,096 known genes in the livers of irradiated-mice with or without soybean isoflavone treatment. Dye swap approach was employed to control for gene-specific dye bias and quantitative real-time RT-PCR was performed on several genes to validate the cDNA microarray data.

Results

Compared with the control group, 68 genes were up-regulated and 28 genes were down-regulated in mice treated with irradiation alone, whereas only 6 genes were down-regulated and 35 genes were up-regulated in mice treated with soybean isoflavone. Interestingly, some of the down-regulated genes in the irradiated group, such as DNA repair and stress response genes and cytoskeleton-associated genes, which are markers of cellular damage after irradiation, were maintained at close to normal expression levels after soybean isoflavone treatment.

Conclusions

Comparison of gene expression profiles in the livers of irradiated-mice treated with or without soybean isoflavone suggested that soybean isoflavone may be an efficient tool to reverse irradiation damage of the liver through multiple-pathways and also provides important clues to further pursue the molecular mechanisms underlying the radio-protective activity of soybean isoflavone.

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Abbreviations

SI::

soybean isoflavone

MAPK::

mitogen-activate protein kinase

MUK/DLK/ZPK::

Mitogen-activated protein kinase upstream kinase/dual leucine zipper-bearing kinase/leucine-zipper protein kinase

JNK::

c-Jun N-terminal kinase

MBIP::

MUK binding Inhibitory Protein

HERPUD1::

homocysteine-induced endoplasmic reticulum ubiquitin-like domain member 1

HSPA5 (GRP78)::

heat shock 70 kD protein 5 (glucose-regulated protein)

ER::

endoplasmic reticulum

Bok::

Bcl-2-related ovarian killer protein

Dusp16::

dual specificity phosphatase 16

Fdft1::

farnesyl diphosphate farnesyl transferase 1

Nfyb::

nuclear transcription factor-Y beta

Btg2::

B-cell translocation gene 2, anti-proliferative

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Acknowledgement

This work was supported by grants from Natural Science Foundation of Shanghai (No.04ZR14083) and the National Natural Science Foundation of China (No. 30271167)

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Authors and Affiliations

  1. Dept. of food science and engineering, Shanghai JiaoTong University, Shanghai, China

    Li-hua Song

  2. Institute of molecular genetics and engineering, Second Military Medical University, Shanghai, China

    Hong-li Yan

  3. Clinical Nutriology Laboratory, Changhai Hospital, Second Military Medical University, 174 ChangHai Road, 200433, Shanghai, China

    Li-hua Song & Dong-lian Cai

Authors
  1. Li-hua Song
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  2. Hong-li Yan
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  3. Dong-lian Cai
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Corresponding author

Correspondence to Dong-lian Cai.

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Li-hua Song and Hong-li Yan contributed equally to this study

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Song, Lh., Yan, Hl. & Cai, Dl. Gene expression profiles in the liver of mice irradiated with 60Co gamma rays and treated with soybean isoflavone. Eur J Nutr 45, 406–417 (2006). https://doi.org/10.1007/s00394-006-0614-y

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  • DOI: https://doi.org/10.1007/s00394-006-0614-y

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