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Oxidative DNA damage and β-catenin expression in colorectal cancer evolution

  • Original Article
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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

Oxidative DNA damage is one of the mechanisms associated to initial colorectal carcinogenesis, but how it interacts with β-catenin, an adherence protein related to cancer evolution, is not clear. This study investigates the relationship between oxidative DNA damage and β-catenin expression in normal mucosa and colon tumor tissue (adenoma and adenocarcinoma) in colorectal adenocarcinoma evolution.

Method

One hundred and 13 samples were studied. Hematoxylin–eosin determined histological grade. β-Catenin expression was analyzed by immunohistochemistry. The oxidative DNA damage was evaluated using comet assay technique. The coefficient for rejection of the nullity hypothesis was taken to 5 %. Kruskal–Wallis, Spearman test, and partial correlation were used to analyze the data.

Results

There was oxidative DNA damage increase in colorectal cancer evolution (p < 0.01). Histological grade was correlated with oxidative DNA damage (p < 0.01). There were differences in β-catenin expression among normal, adenoma, and adenocarcinoma tissue with progressive increase of β-catenin expression (p < 0.00). Histological grade was correlated to β-catenin expression (p < 0.00). There was a relationship (p < 0.00) between β-catenin and histological grade while controlling for the effect of oxidative DNA damage.

Conclusion

The findings of this study make it possible to establish a relationship between oxidative DNA damage and β-catenin expression in normal mucosa and colorectal tumor tissue. Additionally, they show a causal relationship between variations of β-catenin in different tissues analyzed while controlling for the effect of oxidative DNA damage.

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Acknowledgments

The authors acknowledge FAPESP—São Paulo Research Foundation, process no. 2007/01196-5.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Postgraduate Program Strictu Senso in Health Science, Sao Francisco University Medical School, Sao Paulo, Brazil

    Denise G. Priolli & Marcelo L. Ribeiro

  2. Sao Francisco University Medical School, Sao Paulo, Brazil

    Thamy P. Canelloi, Camila O. Lopes, Júlio C. M. Valdívia, Natalia P. Martinez & Demetrius P. Açari

  3. Department of Pathology, Sao Francisco University Medical School, Sao Paulo, Brazil

    Izilda A. Cardinalli

  4. Rua São Vicente 614, Jardim Paulista, Atibaia, São Paulo, Brazil, CEP 12947-390

    Denise G. Priolli

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  1. Denise G. Priolli
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  2. Thamy P. Canelloi
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  8. Marcelo L. Ribeiro
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Corresponding author

Correspondence to Denise G. Priolli.

Additional information

This work was performed within the Postgraduate Program Strictu Senso in Health Science, Sao Francisco University Medical School, Sao Paulo, Brazil.

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Priolli, D.G., Canelloi, T.P., Lopes, C.O. et al. Oxidative DNA damage and β-catenin expression in colorectal cancer evolution. Int J Colorectal Dis 28, 713–722 (2013). https://doi.org/10.1007/s00384-013-1688-7

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  • DOI: https://doi.org/10.1007/s00384-013-1688-7

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