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In vivo regeneration of bladder muscular wall using decellularized colon matrix: an experimental study

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Abstract

Background

Finding a proper scaffold for augmentation is a serious challenge in bladder tissue engineering. We hereby aimed to determine the histological aspects of a decellularized colon graft for bladder augmentation in healthy rats.

Methods

Rat colon tissues were decellularized using perfusion-based method. After partial cystectomy, bladders were grafted with a patch of decellularized colon. Bladder specimens were investigated in 12 rats at 1, 3, and 9 months postoperatively for further histological changes and immunohistochemistry analyses were also performed.

Results

One month after implantation, partial seeding of new cells was observed. After 3 months continuity of transitional epithelium of natural bladder on the decellularized grafted colon tissue was confirmed with histological and immunohistochemical examinations. All augmented bladders demonstrated a spherical shape without stone formation, necrosis or graft rejection. The presence of urothelium with similar morphology to the natural urothelium and visible blood vessels were found within 3 months of operation. All immunohistochemical markers (except markers of colonic stem cells) were expressed in biopsies obtained 3 months after surgery demonstrating a progressive vascular and smooth muscle cell infiltration into the graft after implantation.

Conclusion

This study suggests that decellularized colon may provide a viable material for bladder augmentation in rats to pave the road for future applications of this natural collagen scaffold.

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Abbreviations

α-SMA:

α-Smooth muscle actin

BAMG:

Bladder acellular matrix graft

ECM:

Extra cellular matrix

H&E:

Hematoxylin and eosin

IHC:

Immunohistochemical

PBS:

Phosphate buffered saline

SDS:

Sodium dodecyl sulfate

sGAG:

Sulfated glycosaminoglycan

SIS:

Small intestinal submucosa

RQ-TRAP:

Real-time quantitative telomerase repeat amplification protocol

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Authors and Affiliations

  1. Pediatric Urology Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children’s Hospital Medical Center, Pediatrics Center of Excellence, Tehran University of Medical Sciences, No. 62, Dr. Gharib’s Street, Keshavarz Blvd, Tehran, 1419433151, Iran

    Abdol-Mohammad Kajbafzadeh, Reza Khorramirouz, Shabnam Sabetkish, Mahba Ataei Talebi, Aram Akbarzadeh & Sorena Keihani

Authors
  1. Abdol-Mohammad Kajbafzadeh
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  2. Reza Khorramirouz
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  3. Shabnam Sabetkish
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  4. Mahba Ataei Talebi
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  5. Aram Akbarzadeh
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  6. Sorena Keihani
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Corresponding author

Correspondence to Abdol-Mohammad Kajbafzadeh.

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The authors declare no conflict of interest.

Funding

This work received no funding from any extra-institutional or commercial source.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed. Principles of laboratory animal care were followed and the Animal Ethics Committee of the Tehran University of Medical Sciences, School of Medicine and Education Section of Basic Sciences, approved the animal selection, managements and surgical protocols.

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Kajbafzadeh, AM., Khorramirouz, R., Sabetkish, S. et al. In vivo regeneration of bladder muscular wall using decellularized colon matrix: an experimental study. Pediatr Surg Int 32, 615–622 (2016). https://doi.org/10.1007/s00383-016-3871-8

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  • DOI: https://doi.org/10.1007/s00383-016-3871-8

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