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Acceleration of osteogenesis by platelet-rich plasma with acellular dermal matrix in a calvarial defect model

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Abstract

Purpose

The effects of platelet-rich plasma (PRP) on improvement of bone regeneration have been widely investigated. However, use of a proper scaffold is also important to the delivery of bioactive materials. The aim of this study was to analyze the effects of PRP with acellular dermal matrix (ADM) as a bone substitute in a cranial defect model.

Methods

Twenty-nine New Zealand white rabbits were randomly divided into four groups including control, PRP, ADM, and PRP with ADM. A 15 × 15-mm2 bony defect was carefully created in the cranium. The experimental materials were applied in each group. After 16 weeks, the volume and surface area of new bone were measured using three-dimensional computed tomography and digital photography, and the new bone was analyzed histologically.

Results

The PRP with ADM group exhibited a statistically significant increase in volume and surface area of newly formed bone compared with the other groups (p < 0.05). Histological findings revealed compact lamellar bone in the PRP with ADM group. In contrast, scattered bone islands were detected within the fibrous connective tissue in the other groups.

Conclusions

The regeneration of mature and augmented bone was achieved following the application of PRP mixed with ADM. The results indicated that the PRP combined with ADM could be a potentially useful substitute for bone and may aid in the elimination of additional donor-site harvest procedures.

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Acknowledgments

This work was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (No. NRF-2012R1A1A1006572, IS Yun).

Author contributions

IS Yun performed the experiment, analyzed the data, and wrote a draft of the manuscript. WH Jeong analyzed the data, wrote sections of the manuscript, and edited the figures; these contributions were commensurate with those of IS Yun. BK Jung assisted with the experiment. EH Kang assisted with the experiment and analyzed the data. YS Kim, WJ Lee, TS Roh, and DH Lew supervised the progress of the manuscript.

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Authors and Affiliations

  1. Department of Plastic and Reconstructive Surgery, Keimyung University School of Medicine, Dongsan Medical Center, Daegu, Korea

    Woon Hyeok Jeong

  2. Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University Health System, Gangnam Severance Hospital, 211 Eonju-ro, Gangnam-gu, Seoul, 135-720, Korea

    Tai Suk Roh, Young Seok Kim & In Sik Yun

  3. Department of Plastic and Reconstructive Surgery, Institute for Human Tissue Restoration, Yonsei University Health System, Severance Hospital, Seoul, Korea

    Eun Hye Kang, Bok Ki Jung, Won Jai Lee & Dae Hyun Lew

Authors
  1. Woon Hyeok Jeong
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  2. Tai Suk Roh
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  3. Young Seok Kim
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  4. Eun Hye Kang
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  5. Bok Ki Jung
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  6. Won Jai Lee
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  7. Dae Hyun Lew
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  8. In Sik Yun
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Corresponding author

Correspondence to In Sik Yun.

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Funding

This study was funded by the National Research Foundation of Korea (NRF) funded by the Korean government (No. NRF-2012R1A1A1006572, IS Yun).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

The experimental protocol was approved by the Yonsei University Animal Care and Experiment Committee (2013-0408).

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Cite this article

Jeong, W.H., Roh, T.S., Kim, Y.S. et al. Acceleration of osteogenesis by platelet-rich plasma with acellular dermal matrix in a calvarial defect model. Childs Nerv Syst 32, 1653–1659 (2016). https://doi.org/10.1007/s00381-016-3150-y

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  • DOI: https://doi.org/10.1007/s00381-016-3150-y

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