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Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells

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Abstract

The differentiation rate of adipose-derived mesenchymal stem cells (Ad-MSCs) into endothelial cells is always lower under normal condition, which limits further clinical application of Ad-MSCs for angiogenesis regenerative medicine and needs to be enhanced. In the present study, the tissue-specific-derived decellularized ovine arteries matrix (DCS) was used as scaffold to investigate the pro-endothelial differentiation ability of decellularized ovine arteries matrix as well as the underlying mechanisms. The prepared decellularized ovine arteries matrix by the combination of enzymatic and chemical decellularization approaches preserved macroscopic 3D architecture, native composition and ultrastructure of natural ovine arteries. The RT-PCR, histopathological and immunofluorescence assay results suggested that DCS could increase the proliferation ability of MSC. What’s more, the DCS could also induce the endothelial differentiation of MSC, which was further enhanced by adding VEGF. Our results showed that natural 3D matrix from decellularized ovine arteries could induce the endothelial differentiation of AD-MSCs alone or with the combination of VEGF. Our results indicated that the decellularized ovine arteries matrix would serve as an efficient culture system for promoting endothelial differentiation of Ad-MSCs.

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Authors and Affiliations

  1. Department of Nephrology, The General Hospital of Beijing Military District, Beijing, 100700, People’s Republic of China

    Wenbo Zhang, Yanhong Huo, Yingmin Jia, Li Su, Caixia Wang, Ying Li, Yonghong Yang & Yuanyuan Liu

  2. Department of Information, The General Hospital of Beijing Military District, Beijing, 100700, People’s Republic of China

    Xinling Wang

Authors
  1. Wenbo Zhang
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  2. Yanhong Huo
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  3. Xinling Wang
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  4. Yingmin Jia
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  5. Li Su
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  6. Caixia Wang
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  7. Ying Li
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  8. Yonghong Yang
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  9. Yuanyuan Liu
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Correspondence to Wenbo Zhang.

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This research was supported in part by the Young Scientist Project program (Grant No. 13QNP011).

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The authors have no conflict of interest to disclose.

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Zhang, W., Huo, Y., Wang, X. et al. Decellularized ovine arteries as biomatrix scaffold support endothelial of mesenchymal stem cells. Heart Vessels 31, 1874–1881 (2016). https://doi.org/10.1007/s00380-016-0834-x

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  • DOI: https://doi.org/10.1007/s00380-016-0834-x

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