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Bacterial alkaline phosphomonoesterase in the rhizospheres of plants grown in Chilean extreme environments

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Abstract

Bacterial alkaline phosphomonoesterases (APases) are relevant for organic phosphorus (Po) recycling in many soils. However, the abundance and diversity of bacterial APase in the rhizospheres of native plants are poorly known, particularly in extreme environments. In this research work, we studied the composition of total and APase-harboring bacterial communities, abundances of selected APase genes (phoD and phoX), and APase activities in rhizosphere soils from native plants grown in extreme environments of northern (Atacama Desert), central (Andes volcano; Quetrupillan and Mamuil Malal) and hot spring (Liquiñe), and southern polar (Patagonia and Antarctic) regions of Chile. Differences in the composition of bacterial communities in the rhizosphere soils were revealed by denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) of 16S ribosomal RNA (rRNA), phoD, and phoX genes. In general, the significant lowest bacterial diversities, APase gene abundances, and APase activities were observed in rhizosphere soils from Atacama Desert, whereas the highest values were observed in rhizosphere soils of Patagonia. In addition, APase gene abundances were positively correlated among them and with APase activity of rhizosphere soils, but negatively correlated with phosphorus (P) availability in rhizosphere soils. Although bacterial APases were observed in all studied rhizosphere soils, their relevance to soil Po recycling in soils of extreme environments remains unclear and further studies are needed.

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Acknowledgments

This study was financed by the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT), FONDECYT Postdoctoral Project No. 3140620, FONDECYT Regular Project No. 1120505, and International Cooperation Project Chile-USA code USA2013-0010 from the Chilean Government.

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Authors and Affiliations

  1. Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Ave. Francisco Salazar, 01145, Temuco, Chile

    Jacquelinne J. Acuña, Paola Durán, María de la Luz Mora & Milko A. Jorquera

  2. Programa de Doctorado en Ciencias de Recursos Naturales, Universidad de La Frontera, Ave. Francisco Salazar, 01145, Temuco, Chile

    Lorena M. Lagos

  3. Soil and Water Science Department, University of Florida, 2181 McCarty Hall, PO Box 110290, Gainesville, FL, 32611, USA

    Andrew Ogram

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  1. Jacquelinne J. Acuña
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  2. Paola Durán
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Correspondence to Jacquelinne J. Acuña.

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Acuña, J.J., Durán, P., Lagos, L.M. et al. Bacterial alkaline phosphomonoesterase in the rhizospheres of plants grown in Chilean extreme environments. Biol Fertil Soils 52, 763–773 (2016). https://doi.org/10.1007/s00374-016-1113-9

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