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Nitrite behavior accounts for the nitrous oxide peaks following fertilization in a fluvo-aquic soil

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Abstract

It has been widely demonstrated—both in the field and the laboratory—that N fertilization stimulates peaks in nitrous oxide (N2O) emissions in agricultural soils. However, the mechanisms responsible for this phenomenon remain unclear. In this study, three aerobic incubation experiments were designed to: (1) evaluate the effects of urea and nitrification inhibitors (NIs) on inorganic N concentrations and N2O emissions; (2) establish the relationship between nitrite concentration and N2O emission by adding different amounts of nitrite to soil, and identify the contributions of abiotic processes to N2O production from nitrite by sterilization; and (3) explore the underlying reasons for nitrite accumulation by using 15N tracer methods. Compared with NI treatments, substantial nitrite accumulated in the UREA treatment, mainly attributed to the inhibitory effects of high ammonium from urea hydrolysis on transformation of 15N-nitrite to 15N-nitrate. N2O emission from soil was related to soil nitrite concentration, according to the Michaelis–Menten relationship (R 2 = 0.998; P < 0.01). No significant N2O emission was observed in sterilized soil, indicating that N2O production was a microbial process with the probable dominance of nitrifier denitrification (ND). In conclusion, our results suggest that ammonium fertilizer stimulates nitrite accumulation by inhibiting nitrite transformation to nitrate, thus resulting in an increase in N2O emissions, while NIs reduce N2O emissions by suppressing nitrite formation.

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References

  • Abbasi MK, Shah Z, Adams WA (2003) Effect of the nitrification inhibitor nitrapyrin on the fate of nitrogen applied to a soil incubated under laboratory conditions. J Plant Nutr Soil Sci 166:513–518

    Article  CAS  Google Scholar 

  • Akiyama H, Yan X, Yagi K (2010) Evaluation of effectiveness of enhanced-efficiency fertilizers as mitigation options for N2O and NO emissions from agricultural soils: meta-analysis. Glob Chang Biol 16:1837–1846

    Article  Google Scholar 

  • Azam F, Müller C, Weiske A, Benckiser G, Ottow J (2002) Nitrification and denitrification as sources of atmospheric nitrous oxide—role of oxidizable carbon and applied nitrogen. Biol Fertil Soils 35:54–61

    Article  CAS  Google Scholar 

  • Bateman EJ, Baggs EM (2005) Contributions of nitrification and denitrification to N2O emissions from soils at different water-filled pore space. Biol Fertil Soils 41:379–388

    Article  CAS  Google Scholar 

  • Bouwman A, Boumans L, Batjes N (2002) Emissions of N2O and NO from fertilized fields: summary of available measurement data. Glob Biogeochem Cycles 16:6-1–6-13

    Google Scholar 

  • Bremner JM (1997) Sources of nitrous oxide in soils. Nutr Cycl Agroecosyst 49:7–16

    Article  CAS  Google Scholar 

  • Burns LC, Stevens RJ, Laughlin RJ (1996) Production of nitrite in soil by simultaneous nitrification and denitrification. Soil Biol Biochem 28:609–616

    Article  CAS  Google Scholar 

  • Butterbach-Bahl K, Baggs EM, Dannenmann M, Kiese R, Zechmeister-Boltenstern S (2013) Nitrous oxide emissions from soils: how well do we understand the processes and their controls? Phil Trans R Soc B 368:20130122

    Article  PubMed Central  PubMed  Google Scholar 

  • Cavigelli MA, Del Grosso SJ, Liebig MA, Snyder CS, Fixen PE, Venterea RT, Leytem AB, McLain JE, Watts DB (2012) US agricultural nitrous oxide emissions: context, status, and trends. Front Ecol Environ 10:537–546

    Article  Google Scholar 

  • Chen D, Suter HC, Islam A, Edis R (2010) Influence of nitrification inhibitors on nitrification and nitrous oxide (N2O) emission from a clay loam soil fertilized with urea. Soil Biol Biochem 42:660–664

    Article  CAS  Google Scholar 

  • Cui F, Yan G, Zhou Z, Zheng X, Deng J (2012) Annual emissions of nitrous oxide and nitric oxide from a wheat–maize cropping system on a silt loam calcareous soil in the North China Plain. Soil Biol Biochem 48:10–19

    Article  CAS  Google Scholar 

  • Cui P, Fan F, Yin C, Li Z, Song A, Wan Y, Liang Y (2013) Urea- and nitrapyrin-affected N2O emission is coupled mainly with ammonia oxidizing bacteria growth in microcosms of three typical Chinese arable soils. Soil Biol Biochem 66:214–221

    Article  CAS  Google Scholar 

  • Davidson EA (2009) The contribution of manure and fertilizer nitrogen to atmospheric nitrous oxide since 1860. Nat Geosci 2:659–662

    Article  CAS  Google Scholar 

  • Ding WX, Cai Y, Cai ZC, Yagi K, Zheng XH (2007) Nitrous oxide emissions from an intensively cultivated maize-wheat rotation soil in the North China Plain. Sci Total Environ 373:501–511

    Article  CAS  PubMed  Google Scholar 

  • Florio A, Clark IM, Hirsch PR, Jhurreea D, Benedetti A (2014) Effects of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on abundance and activity of ammonia oxidizers in soil. Biol Fertil Soils 50:795–807

    Article  CAS  Google Scholar 

  • Huang T, Gao B, Hu XK, Lu X, Well R, Christie P, Bakken LR, Ju XT (2014) Ammonia-oxidation as an engine to generate nitrous oxide in an intensively managed calcareous fluvo-aquic soil. Sci Rep 4:3950

    PubMed Central  PubMed  Google Scholar 

  • Ju XT, Xing GX, Chen XP, Zhang SL, Zhang LJ, Liu XJ, Cui ZL, Yin B, Christie P, Zhu ZL (2009) Reducing environmental risk by improving N management in intensive Chinese agricultural systems. Proc Natl Acad Sci U S A 106:3041–3046

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kanter D, Mauzerall DL, Ravishankara A, Daniel JS, Portmann RW, Grabiel PM, Moomaw WR, Galloway JN (2013) A post-Kyoto partner: considering the stratospheric ozone regime as a tool to manage nitrous oxide. Proc Natl Acad Sci U S A 110:4451–4457

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Knowles R (1982) Denitrification. Microbiol Rev 46:43

    CAS  PubMed Central  PubMed  Google Scholar 

  • Kool DM, Dolfing J, Wrage N, Van Groenigen JW (2011) Nitrifier denitrification as a distinct and significant source of nitrous oxide from soil. Soil Biol Biochem 43:174–178

    Article  CAS  Google Scholar 

  • Li J, Shi Y, Luo J, Zaman M, Houlbrooke D, Ding W, Ledgard S, Ghani A (2014) Use of nitrogen process inhibitors for reducing gaseous nitrogen losses from land-applied farm effluents. Biol Fertil Soils 50:133–145

    Article  Google Scholar 

  • Ma Y, Sun L, Zhang X, Yang B, Wang J, Yin B, Yan X, Xiong Z (2013) Mitigation of nitrous oxide emissions from paddy soil under conventional and no-till practices using nitrification inhibitors during the winter wheat-growing season. Biol Fertil Soils 49:627–635

    Article  CAS  Google Scholar 

  • Maharjan B, Venterea RT (2013) Nitrite intensity explains N management effects on N2O emissions in maize. Soil Biol Biochem 66:229–238

    Article  CAS  Google Scholar 

  • McCarty GW (1999) Modes of action of nitrification inhibitors. Biol Fertil Soils 29:1–9

    Article  CAS  Google Scholar 

  • Morrill L, Dawson J (1967) Patterns observed for the oxidation of ammonium to nitrate by soil organisms. Soil Sci Soc Am J 31:757–760

    Article  CAS  Google Scholar 

  • Mosier A, Duxbury J, Freney J, Heinemeyer O, Minami K (1996) Nitrous oxide emissions from agricultural fields: assessment, measurement and mitigation. Plant Soil 181:95–108

    Article  CAS  Google Scholar 

  • Müller C, Laughlin RJ, Spott O, Rütting T (2014) Quantification of N2O emission pathways via a 15N tracing model. Soil Biol Biochem 72:44–54

    Article  Google Scholar 

  • Rütting T, Clough TJ, Müller C, Lieffering M, Newton PC (2010) Ten years of elevated atmospheric carbon dioxide alters soil nitrogen transformations in a sheep - grazed pasture. Glob Chang Biol 16:2530–2542

    Google Scholar 

  • Shcherbak I, Millar N, Robertson GP (2014) Global meta-analysis of the nonlinear response of soil nitrous oxide (N2O) emissions to fertilizer nitrogen. Proc Natl Acad Sci U S A 111:9199–9204

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Shen QR, Ran W, Cao ZH (2003) Mechanisms of nitrite accumulation occurring in soil nitrification. Chemosphere 50:747–753

    Article  CAS  PubMed  Google Scholar 

  • Sistani KR, Jn-Baptiste M, Lovanh N, Cook KL (2011) Atmospheric emissions of nitrous oxide, methane, and carbon dioxide from different nitrogen fertilizers. J Environ Qual 40:1797–1805

    Article  CAS  PubMed  Google Scholar 

  • Slangen J, Kerkhoff P (1984) Nitrification inhibitors in agriculture and horticulture: a literature review. Fertil Res 5:1–76

    Article  CAS  Google Scholar 

  • Stange CF, Spott O, Müller C (2009) An inverse abundance approach to separate soil nitrogen pools and gaseous nitrogen fluxes into fractions related to ammonium, nitrate and soil organic nitrogen. Eur J Soil Sci 60:907–915

    Article  CAS  Google Scholar 

  • Stange CF, Spott O, Russow R (2013) Analysis of the coexisting pathways for NO and N2O formation in Chernozem using the 15N-tracer SimKIM-Advanced model. Isot Environ Health Stud 49:503–519

    Article  CAS  Google Scholar 

  • Stevens R, Laughlin R (1994) Determining nitrogen-15 in nitrite or nitrate by producing nitrous oxide. Soil Sci Soc Am J 58:1108–1116

    Article  CAS  Google Scholar 

  • Syakila A, Kroeze C (2011) The global nitrous oxide budget revisited. Greenh Gas Meas Manag 1:17–26

    Article  CAS  Google Scholar 

  • USDA (1994) Keys to soil taxonomy. United States Department of Agriculture, Soil Conservation Service, 6th ed, pp. 306

  • VanCleemput O, Samater AH (1996) Nitrite in soils: accumulation and role in the formation of gaseous N compounds. Fertil Res 45:81–89

    Article  Google Scholar 

  • Venterea RT (2007) Nitrite-driven nitrous oxide production under aerobic soil conditions: kinetics and biochemical controls. Glob Chang Biol 13:1798–1809

    Article  Google Scholar 

  • Venterea RT, Rolston DE (2000) Mechanisms and kinetics of nitric and nitrous oxide production during nitrification in agricultural soil. Glob Chang Biol 6:303–316

    Article  Google Scholar 

  • Wan Y, Ju X, Ingwersen J, Schwarz U, Stange CF, Zhang F, Streck T (2009) Gross nitrogen transformations and related nitrous oxide emissions in an intensively used calcareous. Soil Sci Soc Am J 73:102

    Article  CAS  Google Scholar 

  • World Meteorological Organization (2012) WMO Greenhouse gas bulletin: the state of greenhouse gases in the atmosphere based on Chinese and global observations through 2011. See http://www.wmo.int/gaw/

  • Wrage N, Velthof GL, van Beusichem ML, Oenema O (2001) Role of nitrifier denitrification in the production of nitrous oxide. Soil Biol Biochem 33:1723–1732

    Article  CAS  Google Scholar 

  • Wuebbles DJ (2009) Atmosphere. Nitrous oxide: no laughing matter. Science 326:56–57

    Article  CAS  PubMed  Google Scholar 

  • Yan X, Akimoto H, Ohara T (2003) Estimation of nitrous oxide, nitric oxide and ammonia emissions from croplands in East, Southeast and South Asia. Glob Chang Biol 9:1080–1096

    Article  Google Scholar 

  • Yan X, Hosen Y, Yagi K (2001) Nitrous oxide and nitric oxide emissions from maize field plots as affected by N fertilizer type and application method. Biol Fertil Soils 34:297–303

    Article  CAS  Google Scholar 

  • Yang J, Li X, Xu L, Hu F, Li H, Liu M (2013) Influence of the nitrification inhibitor DMPP on the community composition of ammonia-oxidizing bacteria at microsites with increasing distance from the fertilizer zone. Biol Fertil Soils 49:23–30

    Article  CAS  Google Scholar 

  • Zhang J, Cai Z, Zhu T (2011) N2O production pathways in the subtropical acid forest soils in China. Environ Res 111:643–649

    Article  CAS  PubMed  Google Scholar 

  • Zhu X, Burger M, Doane TA, Horwath WR (2013) Ammonia oxidation pathways and nitrifier denitrification are significant sources of N2O and NO under low oxygen availability. Proc Natl Acad Sci U S A 110:6328–6333

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Professor Paolo Nannipieri and two anonymous reviewers for their valuable comments and helpful suggestions on the original manuscript. This study was financially supported by the “Strategic Priority Research Program—Climate Change: Carbon Budget and Relevant Issues” of the Chinese Academy of Sciences (No. XDA05020200), and the National Natural Science Foundation of China (No. 41201221). The authors declare no competing financial interests.

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Authors and Affiliations

  1. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China

    Lan Ma, Jun Shan & Xiaoyuan Yan

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Lan Ma

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  1. Lan Ma
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Correspondence to Xiaoyuan Yan.

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Ma, L., Shan, J. & Yan, X. Nitrite behavior accounts for the nitrous oxide peaks following fertilization in a fluvo-aquic soil. Biol Fertil Soils 51, 563–572 (2015). https://doi.org/10.1007/s00374-015-1001-8

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