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Na+/K+-ATPase response to salinity change and its correlation with FXYD11 expression in Anguilla marmorata

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Abstract

The Na+/K+-ATPase (NKA) is a primary electrogenic protein that promotes ion transport in teleosts. FXYD11 is a putative regulatory subunit of the NKA pump. The regulation of Na +/K + -ATPase and FXYD11 is of critical importance for osmotic homeostasis. To investigate the changes of the two genes under different salinity environments, we first identified NKA (AmNKAα1) and FXYD11 (AmFXYD11) in Anguilla marmorata, and then evaluated the mRNA levels of NKA and FXYD11 as well as the activity of NKA in the gill and kidney at different timepoints (0, 1, 3, 6, 12, 24, 48, 72, 96, and 360 h) under three salinity conditions-0‰ (fresh water: FW), 10‰ (brackish water: BW), and 25‰ (seawater: SW). In the gill, the mRNA levels of AmNKAα1 and AmFXYD11 and the enzyme activity of AmNKAα1 were higher in BW and SW than in FW; the protein abundance was positively correlated with the specific activity of NKA in BW/SW. However, in the kidney, the mRNA level of AmNKAα1 in the BW group was higher than that in the FW group. In addition, AmFXYD mRNA levels in both BW and SW groups were significantly lower than that in the FW control group. These results suggested that AmFXYD11 was tissue specific in response to different salinity environment. Our results clearly demonstrated the important roles of AmNKAα1 and AmFXYD11 in osmotic homeostasis of juvenile A. marmorata under saline environment.

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Abbreviations

NKAα1 :

Na+/K+-ATPase α1

FW:

Fresh water

BW:

Brackish water

SW:

Seawater

cDNA:

Complementary deoxyribonucleic acid

RACE:

Rapid amplification of cDNA ends

BLAST:

Basic local alignment search tool

NCBI:

National Center Manuscript for Biotechnology Information

ORF:

Open reading frame

UTR:

Untranslated region(s)

qPCR:

Fluorescent real-time quantitative polymerase chain reaction

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Acknowledgements

This study was supported by the National Natural Science of Jiangsu Province (BK20141450), the National Natural Science Foundation of China (30770283), Project Foundation of the Academic Program Development of Jiangsu Higher Education Institution (PAPD), and the Innovation of Graduate Student Training Project of Jiangsu Province (CXLX13-381).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China

    Fenfei Liang, Li Li, Guosong Zhang, Shaowu Yin, Xiaolu Wang, Peng Li, Yihe Jia, Yayuan Wang, Li Wang & Xiaojun Wang

  2. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, 222005, China

    Fenfei Liang, Li Li, Guosong Zhang, Shaowu Yin, Xiaolu Wang, Peng Li, Yihe Jia, Yayuan Wang, Li Wang & Xiaojun Wang

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  1. Fenfei Liang
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  9. Li Wang
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Correspondence to Shaowu Yin.

Additional information

Communicated by G. Heldmaier.

F. Liang and L. Li contributed equally to this work.

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Liang, F., Li, L., Zhang, G. et al. Na+/K+-ATPase response to salinity change and its correlation with FXYD11 expression in Anguilla marmorata . J Comp Physiol B 187, 973–984 (2017). https://doi.org/10.1007/s00360-017-1059-8

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