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Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts

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Abstract

Cardiac function in fish acclimates to long-term temperature shifts by generating compensatory changes in structure and function of sarcoplasmic reticulum (SR) including the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA2). The current study compares temperature responses of the cardiac SERCA in two fish species, burbot (Lota lota) and crucian carp (Carassius carassius), which differ in regard to thermal tolerance and activity pattern. Burbot are cold stenothermal and cold-active, while crucian carp are eurythermal and cold-dormant. The fish were acclimated at 4 °C (cold-acclimation, CA) or 18 °C (warm-acclimation, WA) and expression of SERCA proteins and transcript was measured from atrium and ventricle. Burbot heart expresses one major isoform of SERCA (110 kDa), while crucian carp heart expresses two isoforms (110 and 93 kDa). Expression of SERCA proteins was about four times higher (P < 0.05) in the heart of CA burbot than WA burbot, in both cardiac chambers. In the heart of crucian carp, thermal acclimation did not affect SERCA proteins, in either chamber (P > 0.05). The expression of SERCA transcripts did not follow the expression pattern of SERCA protein in either species, suggesting that SERCA expression is mainly regulated posttranscriptionally. These findings show that the stenothermal and cold-active burbot compensates for the decrease in ambient temperature by increasing the expression of SERCA. In the eurythermal and cold-dormant crucian carp SERCA expression is independent of temperature, while the presence of two SERCA isoforms may provide some thermal independence in SR Ca2+ pumping.

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Acknowledgments

Skillful technical assistance of Anita Kervinen and Riitta Pietarinen is gratefully acknowledged.

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There is no personal or financial conflict of interest in the present study.

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Correspondence to Hanna Korajoki.

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Communicated by G. Heldmaier.

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360_2012_724_MOESM1_ESM.ai

Supplementary Figure 1. The sequence of the reference gene DnaJA2 mRNA (Gen Bank number JX193859) cloned from the cold-acclimated (+4°C) burbot (ll, Lota lota L.) atrium. Sequences of DnaJA2 from zebra fish (dr, Danio rerio L., NM_213493.1) and crucian carp (cc, Carassius carassius L., EU191947.1) are shown for comparison. Degenerative primer binding sites (Hassinen et al. 2008) were excluded from the burbot sequence (AI 165 kb)

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Korajoki, H., Vornanen, M. Temperature dependence of sarco(endo)plasmic reticulum Ca2+ ATPase expression in fish hearts. J Comp Physiol B 183, 467–476 (2013). https://doi.org/10.1007/s00360-012-0724-1

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