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Changes in diet, body mass and fatty acid composition during pre-hibernation in a subtropical bat in relation to NPY and AgRP expression

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Abstract

Prior to hibernation, mammals accumulate large amounts of fat in their bodies. In temperate mammalian species, hibernation is improved by increasing the levels of poly-unsaturated fatty acids (PUFA) in the body. The saturation of fatty acids (FA) in both white adipose tissue (WAT) and membrane phospholipids of mammals often reflects their diet composition. We found that the greater mouse-tailed bat (Rhinopoma microphyllum) accumulates large amounts of fat at the end of summer by gradually shifting to a fat-rich diet (queen carpenter ants, Camponotus felah). PUFA are almost absent in this diet (<1 % of total FA), which contains a high fraction of saturated (SFA) and mono-unsaturated (MUFA) fatty acids. We found similar low levels of PUFA in mouse-tailed bat WAT, but not in their heart total lipids. The expression of two appetite-stimulating (orexigenic) hypothalamic neuropeptides, AgRP and NPY, increased in parallel to the shift in diet and with fat gain in these bats. To the best of our knowledge, this is the only documented example of specific pre-hibernation diet in bats, and one which reveals the most saturated FA composition ever documented in a mammal. We suggest that the increase in expression levels of NPY and AgRP may contribute to the observed diet shift and mass gain, and that the FA composition of the bat’s specialized diet is adaptive in the relatively high temperatures we recorded in both their winter and summer roosts.

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Acknowledgments

We would like to thank Yotam Shenaar, Rotem Cohen-Paz, Orly Barak, Osnat Malka, and Tovit Simon for help with the laboratory work; Luzie Braulke for reading and comments on the manuscript; and Naomi Paz for editing the manuscript. This research was supported by The Israel Science Foundation (Grant No. 232/08).

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Authors and Affiliations

  1. Department of Zoology, Tel-Aviv University, 69778, Tel-Aviv, Israel

    Eran Levin, Yoram Yom-Tov, Abraham Hefetz & Noga Kronfeld-Schor

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  1. Eran Levin
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  2. Yoram Yom-Tov
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  3. Abraham Hefetz
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  4. Noga Kronfeld-Schor
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Correspondence to Eran Levin.

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Communicated by G. Heldmaier.

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Levin, E., Yom-Tov, Y., Hefetz, A. et al. Changes in diet, body mass and fatty acid composition during pre-hibernation in a subtropical bat in relation to NPY and AgRP expression. J Comp Physiol B 183, 157–166 (2013). https://doi.org/10.1007/s00360-012-0689-0

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