Abstract
Objectives
To investigate the expression level of testis-specific calcium-binding protein CBP86-IV in normal and asthenozoospermic human sperm.
Methods
The total RNA was extracted from human sperm, and target cDNA was obtained by reverse transcription–polymerase chain reaction. Then the cDNA was used for quantitative PCR analysis and cloned into the prokaryotic expression vector pET-28a, respectively. The fusion protein was induced and expressed as inclusion body which was used to produce the polyclonal antibody against TSCBP86-IV. The protein expression level of TSCBP86-IV from normal human sperm and idiopathic asthenozoospermic samples was detected by the purified antibody.
Results
The experimental results showed that the protein expression of TSCBP86-IV was reduced in idiopathic asthenozoospermia and consistent with the transcriptional changing tendency which was detected by quantitative PCR analysis.
Conclusions
The stable and reliable change of TSCBP86-IV may be taken as a new molecular marker for clinical diagnosis of idiopathic asthenozoospermia.
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Acknowledgments
The work was supported by National Natural Science Foundation of China (No. 81260123) and Nature Science Foundation of the Guangxi Zhuang Autonomous Region to Shu-lin Shen (No. 012GXNSFAA053168).
Conflict of interest
The authors declare no conflict of interest.
Ethical standard
The experimental data in this study have been approved by the appropriate ethics committee and performed in accordance with the ethical standards laid down in the Declaration of Helsinki 1964 and its later amendments. All persons gave their informed consent prior to their inclusion. The corresponding authors take on the above responsibility.
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Shen, S., Wang, J., Liang, J. et al. Low-expressed testis-specific calcium-binding protein CBP86-IV (CABYR) is observed in idiopathic asthenozoospermia. World J Urol 33, 633–638 (2015). https://doi.org/10.1007/s00345-015-1516-5
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DOI: https://doi.org/10.1007/s00345-015-1516-5