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Overlap between Signaling Pathways Responsive to Xanthomonas oryzae pv. oryzae Infection and Drought Stress in Rice Introgression Line Revealed by RNA-Seq

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Abstract

Rice bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), and drought stress are common reducers of rice yields in some regions of Asia and Africa. The rice introgression line H471 exhibits broad-spectrum dual resistance to Xoo and drought tolerance. To obtain a deeper understanding of crosstalk between signaling pathways in response to Xoo infection and drought stress, whole-genome transcriptome analyses of H471 and its recurrent parent Huang-Hua-Zhan (HHZ) were performed. A set of significant KEGG pathways involved in dual stress resistance were inferred by comparing H471 and HHZ samples under Xoo infection and drought stress using the gene set enrichment analysis method. We detected 178 genes that were differentially expressed in H471 common in response to the dual stresses when compared with HHZ, including genes for signaling perception and transduction, transcription regulation, and stress response. Putative common co-regulatory networks were constructed using 39 of the differentially expressed genes from the three different functional groups. These results will be a valuable source of information for further investigations on crosstalk between signaling pathways responsive to Xoo infection and drought stress in rice.

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Acknowledgments

This research was supported by the CAAS Innovative Team Award, the National High-tech Program of China (No. 2014AA10A603), the Natural Science Foundation of China (No. 31261140369), the Shenzhen Technology Research & Development (No. JSGG20121026152117750), the Shenzhen Peacock Plan (No. 20130415095710361), and the Bill & Melinda Gates Foundation (No. OPP51587).

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    Authors and Affiliations

    1. Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, 12# South Zhong-Guan-Cun Street, Beijing, 100081, People’s Republic of China

      Fan Zhang, Fan Zhang, Liyu Huang, Jianlong Xu, Yongli Zhou & Zhikang Li

    2. Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, People’s Republic of China

      Jianlong Xu, Yongli Zhou & Zhikang Li

    3. Food Crops Research Institute, Yunnan Academy of Agricultural Sciences, Kunming, 650201, People’s Republic of China

      Liyu Huang

    4. International Rice Research Institute, DAPO Box 7777, Metro Manila, The Philippines

      Casiana Vera Cruz & Jauhar Ali

    5. Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

      Fan Zhang

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    1. Fan Zhang
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    Fan Zhang and Fan Zhang have contributed equally to this work.

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    Zhang, F., Zhang, F., Huang, L. et al. Overlap between Signaling Pathways Responsive to Xanthomonas oryzae pv. oryzae Infection and Drought Stress in Rice Introgression Line Revealed by RNA-Seq. J Plant Growth Regul 35, 345–356 (2016). https://doi.org/10.1007/s00344-015-9538-1

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