Skip to main content
SpringerLink
Log in

Growth and Fv/Fm in embryos of Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) cultured under different temperature and irradiance conditions

  • Published:
Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

Understanding the embryo physiology of Hizikia fusiformis (Harvey) Okamura will promote the development of artificial seedlings. In this study, H. fusiformis embryos were cultured under different environmental conditions, including different temperatures (15°C, 20°C, and 25°C), sunlight intensities (100%, 50%, and 25% of full solar radiation) and types of UVR (UV-A and UV-B). Then, we determined the lengths, maximal quantum yields ( Fv/Fm ), and survival rates of the embryos. The results showed that the embryos had the highest lengths, Fv/Fm values and survival percentages when cultured under a temperature of 20°C and 25% sunlight intensity. The full level of solar radiation severely reduced the growth and survival ratios of the embryos. Hizikia embryos recovered within 2 h from the damage caused by short-term (30 min) high radiation (about 70% of full solar radiation) and UVR exposure. However, the viabilities of the embryos were significantly decreased after 4 h exposure to UVR. According to these results, we propose that culture conditions with indoor natural light and room temperature would be favorable for the growth, development and physiology of Hizikia embryos.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Al–Talib S M, Al–Ghabsha T S, Al–Sabha T N. 2014. Spectrophotometric determination of adrenaline using methyl thiazolyl diphenyl–tetrazolium–bromide(MTT). AL–Taqani, 27(2): 46–53.

    Google Scholar 

  • Altamirano M, Flores–Moya A, Figueroa F L. 2003a. Effects of UV radiation and temperature on growth of germlings of three species of Fucus(Phaeophyceae). Aquatic Botany, 75(1): 9–20.

    Article  Google Scholar 

  • Altamirano M, Flores–Moya A, Kuhlenkamp R, Figueroa F L. 2003b. Stage–dependent sensitivity to ultraviolet radiation in zygotes of the brown alga Fucus serratus. Zygote, 11(2): 101–106.

    Article  Google Scholar 

  • Beer S, Björk M, Beardall J. 2014. Photosynthesis in the Marine Environment. Wiley Blackwell, Oxford.

    Google Scholar 

  • Bruggisser R, Von Daeniken K, Jundt G, Schaffner W, Tullberg–Reinert H. 2002. Interference of plant extracts, phytoestrogens and antioxidants with the MTT tetrazolium assay. Planta Med ica, 68(5): 445–448.

    Article  Google Scholar 

  • Cruces E, Huovinen P, Gómez I. 2013. Interactive effects of UV radiation and enhanced temperature on photosynthesis, phlorotannin induction and antioxidant activities of two Sub–Antarctic brown algae. Marine Biology, 160(1): 1–13.

    Article  Google Scholar 

  • Dring M J, Makarov V, Schoschina E, Lorenz M, Lüning K. 1996. Influence of ultraviolet–radiation on chlorophyll fluorescence and growth in different life–history stages of three species of Laminaria(Phaeophyta). Marine Biology, 126(2): 183–191.

    Article  Google Scholar 

  • Figueroa F L, Jiménez C, Viñegla B, Péter–Rodígueze E, Aguilera J, Flores–Moya A, Altamirano M, Lebert M, Häder D P. 2002. Effects of solar UV radiation on photosynthesis of the marine angiosperm Posidonia oceanica from southern Spain. Marine Ecology Progress Series, 230: 59–70.

    Article  Google Scholar 

  • Gao G, Wu H Y, Gao K S. 2009. Effects of solar ultraviolet radiation on growth and photosynthesis of Hizikia fusiformis. Acta Hydrobiologica Sinica, 33(2): 284–288. (in Chinese with English abstract)

    Article  Google Scholar 

  • Gao K S. 2014. Algal Carbon Fixation. Science Press, Beijing. 405p.(in Chinese)

    Google Scholar 

  • Häder D P, Kumar H D, Smith R C, Worrest R C. 2007. Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochemical & Photobiological Sciences: Official Journal of the European Photochemistry Association and the European Society for Photobiology, 6(3): 267–285.

    Article  Google Scholar 

  • Han Y S, Han T. 2005. UV–B induction of UV–B protection in Ulva pertusa(Chlorophyta). Journal of Phycology, 41(3): 523–530.

    Article  Google Scholar 

  • Henry B E, Van Alstyne K L. 2004. Effects of UV radiation on growth and phlorotannins in Fucus gardneri (Phaeophyceae) juveniles and embryos. Journal of Phycology, 40(3): 527–533.

    Article  Google Scholar 

  • Hiriart–Baer V P, Arciszewski T J, Malkin S Y, Guildford S J, Hecky R E. 2008. Use of pulse–amplitude–modulated fluorescence to assess the physiological status of Cl adophora sp. along a water quality gradient. Journal of Phycology, 44(6): 1 604–1 613.

    Article  Google Scholar 

  • James C E, Davey M W. 2007. A rapid colorimetric assay for the quantitation of the viability of free–living larvae of nematodes in vitro. Parasitol ogy Res earch, 101(4): 975–980.

    Article  Google Scholar 

  • Kitajima M, Butler W L. 1975. Quenching of chlorophyll fluorescence and primary photochemistry in chloroplasts by dibromothymoquinone. Biochimica et Biophysica Acta ( BBA )–Bioenergetics, 376(1): 105–115.

    Article  Google Scholar 

  • Li S Y. 2001 Studies on techneque of industrial raising seedlings of Sargass te m fusiforme(Harvey) setch. Journal of Zhejiang Ocean University( Natural Science ), 20(3): 251–255, 265.(in Chinese with English abstract)

    Google Scholar 

  • Liu S X, Zou D H, Xu J T, Gao K S. 2008. Responses of the brown seaweed Hizikia fusiformis cultured at different N levels to the solar radiation. Marine Science Bulletin, 27(6): 44–51.(in Chinese with English abstract)

    Google Scholar 

  • Liu S X, Zou D H, Xu J T. 2010. Response of the young sporophytes of Hizikia fusiformis to different N growth conditions and the solar radiation. Acta Ecologica Sinica, 30(20): 5 562–5 568.(in Chinese with English abstract)

    Google Scholar 

  • Luo Q J, Li W B. 2002. Breed seedling in door of Hizikia fusiform e from cultivation. Journal of Ningbo University ( NSEE ), 15(4): 34–36.(in Chinese with English abstract)

    Google Scholar 

  • Luo Q J. 2001. An experiment on the culturing seedling at sea of Sargassum fusiforme. setch. Transactions of Oceanology and Limnology,(3): 17–21.(in Chinese with English abstract)

    Google Scholar 

  • Pang S J, Chen L T, Zhuang D G, Fei X G, Sun J Z. 2005. Cultivation of the brown alga Hizikia fusiformis(Harvey) Okamura: enhanced seedling production in tumbled culture. Aquaculture, 245(1–4): 321–329.

    Article  Google Scholar 

  • Pang S J, Fei X G, Xiao T, Wang J C. 2000. Suspended culture of the receptacles of Hizikia fusiformis. Marine Science s, 24(3): 1–3.(in Chinese with English abstract)

    Google Scholar 

  • Pang S J, Fei X G, Xiao T, Wang J G. 2001. Mass production of the artificial seeds of Hizikia fusiformis by controling the laying out of the ovules and semens. Marine Science s, 25(4): 53–54.(in Chinese with English abstract)

    Google Scholar 

  • Pang S J, Gao S Q, Sun J Z. 2006. Cultivation of the brown alga Hizikia fusiformis(Harvey) Okamura: controlled fertilization and early development of seedlings in raceway tanks in ambient light and temperature. Journal of Applied Phycology, 18(6): 723–731.

    Article  Google Scholar 

  • Pang S J, Zhang Z H, Zhao H J, Sun J Z. 2007. Cultivation of the brown alga Hizikia fusiformis(Harvey) Okamura: stress resistance of artificially raised young seedlings revealed by chlorophyll fluorescence measurement. Journal of Applied Phycology, 19(5): 557–565.

    Article  Google Scholar 

  • Pattison D I, Rahmanto A S, Davies M J. 2012. Photo–oxidation of proteins. Photochemical & Photobiological Sciences: Official Journal of the European Photochemistry Association and the European Society for Photobiology, 11(1): 38–53.

    Article  Google Scholar 

  • Pavia H, Brock E. 2000. Extrinsic factors influencing phlorotannin production in the brown alga Ascophyllum nodosum. Marine Ecology Progress Series, 193: 285–294.

    Article  Google Scholar 

  • Peinado N K, Díaz R T A, Figueroa F L, Helbling E W. 2004. Ammonium and UV radiation stimulate the accumulation of mycosporine–like amino acids in Porphyra columbina (Rhodophyta) from Patagonia, Argentina1. Journal of Phycology, 40(2): 248–259.

    Article  Google Scholar 

  • Ragen M A, Glombitza K W. 1986. Phlorotannins, brown algal polyphenols. Progress in Phycological Research, 4: 129–241.

    Google Scholar 

  • Raven J A, Hurd C L. 2012. Ecophysiology of photosynthesis in macroalgae. Photosynthesis Research, 113(1–3): 105–125.

    Article  Google Scholar 

  • Roleda M Y, Hanelt D, Kräbs G, Wiencke C. 2004. Morphology, growth, photosynthesis and pigments in Laminaria ochroleuca(Laminariales, Phaeophyta) under ultraviolet radiation. Phycologia, 43(5): 603–613.

    Article  Google Scholar 

  • Smith R A, Pontiggia L, Waterman C, Lichtenwalner M, Wasserman J. 2009. Comparison of motility, recovery, and methyl–thiazolyl–tetrazolium reduction assays for use in screening plant products for anthelmintic activity. Parasitology Research, 105(5): 1 339–1 343.

    Article  Google Scholar 

  • Swanson A K, Fox C H. 2007. Altered kelp(Laminariales) phlorotannins and growth under elevated carbon dioxide and ultraviolet–B treatments can influence associated intertidal food webs. Global Change Biology, 13(8): 1 696–1 709.

    Article  Google Scholar 

  • Van De Poll W H, Eggert A, Buma A G J, Breeman A M. 2001. Effects of UV–B–induced DNA damage and photoinhibition on growth of temperate marine red macrophytes: habitatrelated differences in UV–B tolerance. Journal of Phycology, 37(1): 30–38.

    Article  Google Scholar 

  • Van De Poll W H, Eggert A, Buma A G J, Breeman A M. 2002. Temperature dependence of UV radiation effects in arctic and temperate isolates of three red macrophytes. European Journal of Phycology, 37(1): 59–68.

    Article  Google Scholar 

  • Véliz K, Edding M, Tala F, Gómez I. 2006. Effects of ultraviolet radiation on different life cycle stages of the South Pacific kelps, Lessonia nigrescens and Lessonia trabeculata (Laminariales, Phaeophyceae). Marine Biology, 149(5): 1 015–1 024.

    Article  Google Scholar 

  • Yan L W, Huang H J, Chen J T, Yang X G. 2011. Estimation of carbon sink capacity of algal mariculture in the coastal areas of China. Advances in Marine Science, 29(4): 537–545.(in Chinese with English abstract)

    Google Scholar 

  • Zeng C K. 2000. Flora Algarum Marinarum Sinicarum. Science Press, Beijing. 238p.(in Chinese)

    Google Scholar 

  • Zhang L N, Luo Q J, Lin S Z, Yan X J. 2012. Seedling culturing of Sargassum fusiforme setch in summer in North Sea. Journal of Ningbo University( NSEE ), 25(4): 6–9.(in Chinese with English abstract)

    Google Scholar 

  • Zhang Z, Liu J G, Liu J D. 2002. Study review of Hizikia fusiform is. Marine Fisheries Research, 23(3): 67–74.(in Chinese with English abstract)

    Google Scholar 

  • Zhao S F, Li H J, Sun H Q, Li J P, Li G R. 2013. Effects of light intensity on sexual reproduction and early development of Sargassum cinereum(Fucales, Phaeophyta) germlings. Journal of Shanghai Ocean University, 22(4): 563–570. (in Chinese with English abstract)

    Google Scholar 

  • Zhao S F, Yao W L, Guo X Z, He T T, Sun H Q, Guo S D, Huang G H. 2015. Combined effects of temperature and light on the growth rate of Hizikia fusiformis young sporophyte. Journal of Aquaculture, 36(10): 42–47.(in Chinese with English abstract)

    Google Scholar 

  • Zhu Z J, Chen P M. 1997. The relationship between water temperature, light intensity and the photosynthetic rates of Sargassum fusiform e. Journal of Fisheries of China, 21(2): 165–170.(in Chinese with English abstract)

    Google Scholar 

  • Zou D H, Gao K S, Chen W Z. 2011. Photosynthetic carbon acquisition in S argassum henslowianum(Fucales, Phaeophyta), with special reference to the comparison between the vegetative and reproductive tissues. Photosynthesis Research, 107(2): 159–168.

    Article  Google Scholar 

  • Zou D H, Gao K S. 2004. Comparative mechanisms of photosynthetic carbon acquisition in Hizikia fusiforme under submersed and emersed conditions. Acta Botanica Sinica, 46(10): 1 178–1 185.

    Google Scholar 

  • Zou D H, Liu S X, Du H, Xu J T. 2012. Growth and photosynthesis in seedlings of Hizikia fusiformis(Harvey) Okamura(Sargassaceae, Phaeophyta) cultured at two different temperatures. Journal of Applied Phycology, 24(5): 1 321–1 327.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China

    Longle Wang  (王龙乐), Dinghui Zou  (邹定辉) & Heng Jiang  (姜恒)

  2. The Science and Technology Division, South China University of Technology, Guangzhou, 510006, China

    Xin Zhang  (张鑫)

  3. The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou, 510006, China

    Dinghui Zou  (邹定辉)

  4. Marine Biology Institute, Science Center, Shantou University, Shantou, 515063, China

    Weizhou Chen  (陈伟洲)

Authors
  1. Longle Wang  (王龙乐)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin Zhang  (张鑫)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dinghui Zou  (邹定辉)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Weizhou Chen  (陈伟洲)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Heng Jiang  (姜恒)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dinghui Zou  (邹定辉).

Additional information

Supported by the Science and Technology Planning Project of Guangdong, China (No. 2016A020222001)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, L., Zhang, X., Zou, D. et al. Growth and Fv/Fm in embryos of Hizikia fusiformis (Harvey) Okamura (Sargassaceae, Phaeophyta) cultured under different temperature and irradiance conditions. J. Ocean. Limnol. 36, 1798–1805 (2018). https://doi.org/10.1007/s00343-018-7055-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00343-018-7055-3

Keyword

Search

Navigation