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Comparison of the longissimus muscle proteome between obese and lean pigs at 180 days

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Abstract

Production of high-quality meat is important to satisfy the consumer and make the pig industry competitive. Obese and lean breeds of pig show clear differences in adipogenic capacity and meat quality, but the underlying molecular mechanism remains unclear. We have compared protein expression of the longissimus muscle between Lantang (LT, obese) and Landrace (LR, lean) pigs at the age of 180 days using two-dimensional fluorescence difference gel electrophoresis. Of the 1,400 protein spots detected per gel, 18 were differentially expressed between the two breeds. Using peptide mass fingerprint and tandem mass spectrometry, 17 protein spots were identified, corresponding to ten different proteins that could be divided into four groups: metabolism-related, structure-related, stress-related, and other (unclassified). Among the metabolism-related proteins, COX5A and ATP5B, which participate in oxidative phosphorylation, were highly expressed in LT, whereas ENO3, which is involved in glycolysis, was highly expressed in LR. These results may contribute valuable information to our understanding of the molecular mechanism responsible for differences between obese and lean pigs, such as growth rate and meat quality.

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Acknowledgments

This research was funded by National High Technology Research and Development Program of China (863 Program, Grant No. 2011AA100304), National Key Technology R&D Program of the Ministry of Science and Technology (Grant No. 2011BAD28B01-01) and China Agriculture Research System (CARS-36).

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Authors and Affiliations

  1. State Key Laboratory of Biocontrol, School of Life Science, Sun Yat-Sen University, Guangzhou, 510006, People’s Republic of China

    Anning Li, Delin Mo, Xiao Zhao, Wei Jiang, Peiqing Cong, Zuyong He, Shuqi Xiao, Xiaohong Liu & Yaosheng Chen

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  1. Anning Li
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  2. Delin Mo
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  3. Xiao Zhao
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  4. Wei Jiang
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  5. Peiqing Cong
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  6. Zuyong He
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  7. Shuqi Xiao
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  8. Xiaohong Liu
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  9. Yaosheng Chen
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Corresponding author

Correspondence to Yaosheng Chen.

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Anning Li and Delin Mo contributed equally to this work.

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Li, A., Mo, D., Zhao, X. et al. Comparison of the longissimus muscle proteome between obese and lean pigs at 180 days. Mamm Genome 24, 72–79 (2013). https://doi.org/10.1007/s00335-012-9440-0

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  • DOI: https://doi.org/10.1007/s00335-012-9440-0

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