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Quantitative trait loci for hip dysplasia in a crossbreed canine pedigree

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Abstract

Canine hip dysplasia is a common developmental inherited trait characterized by hip laxity, subluxation or incongruity of the femoral head and acetabulum in affected hips. The inheritance pattern is complex and the mutations contributing to trait expression are unknown. In the study reported here, 240 microsatellite markers distributed in 38 autosomes and the X chromosome were genotyped on 152 dogs from three generations of a crossbred pedigree based on trait-free Greyhound and dysplastic Labrador Retriever founders. Interval mapping was undertaken to map the QTL underlying the quantitative dysplastic traits of maximum passive hip laxity (the distraction index), the dorsolateral subluxation score, and the Norberg angle. Permutation testing was used to derive the chromosome-wide level of significance at p < 0.05 for each QTL. Chromosomes 4, 9, 10, 11 (p < 0.01), 16, 20, 22, 25, 29 (p < 0.01), 30, 35, and 37 harbor putative QTL for one or more traits. Successful detection of QTL was due to the crossbreed pedigree, multiple-trait measurements, control of environmental background, and marked advancement in canine mapping tools.

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Acknowledgments

This work was supported by the Morris Animal Foundation; Cornell Advanced Technology, Biotechnology; Consolidated Research Grant Program, the Dean’s Fund for Clinical Excellence, College of Veterinary Medicine, Cornell University; NIH grant No. R01 AR47558; Nestle-Purina Inc.; and the NHLBI Mammalian Genotyping Service, Marshfield, WI. The authors thank Jennifer Johnson for assistance with MULTIMAP.

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Authors and Affiliations

  1. Department of Clinical Sciences, Box 32 College of Veterinary Medicine, Cornell University, Ithaca, New York, 14853, USA

    Rory J. Todhunter, Raluca Mateescu, Nathan L. Dykes, Stuart P. Bliss, Margaret Vernier-Singer, Carlos Harjes, Robert O. Gilbert & Dietrich Volkman

  2. James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York, 14853, USA

    George Lust, Nancy I. Burton-Wurster, Alma J. Williams, Elizabeth Corey & Gregory M. Acland

  3. Department of Animal Breeding, College of Agriculture and Life Sciences, Cornell University, Ithaca, New York, 14853, USA

    Richard L. Quaas & Zhiwu Zhang

  4. Department of Statistics, University of Florida, Gainesville, Florida, 32611, USA

    George Casella & Rongling Wu

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  1. Rory J. Todhunter
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  2. Raluca Mateescu
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  3. George Lust
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  9. Elizabeth Corey
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  10. Carlos Harjes
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  11. Richard L. Quaas
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  13. Robert O. Gilbert
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  14. Dietrich Volkman
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  15. George Casella
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  16. Rongling Wu
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  17. Gregory M. Acland
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Correspondence to Rory J. Todhunter.

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Todhunter, R.J., Mateescu, R., Lust, G. et al. Quantitative trait loci for hip dysplasia in a crossbreed canine pedigree. Mamm Genome 16, 720–730 (2005). https://doi.org/10.1007/s00335-005-0004-4

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  • DOI: https://doi.org/10.1007/s00335-005-0004-4

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