Polar Biology

, Volume 37, Issue 3, pp 403–416

Characterization and comparison of potential denitrifiers in microbial mats from King George Island, Maritime Antarctica

  • Rocio J. Alcántara-Hernández
  • Carla M. Centeno
  • Alejandro Ponce-Mendoza
  • Silvia Batista
  • Martin Merino-Ibarra
  • Julio Campo
  • Luisa I. Falcón
Original Paper

DOI: 10.1007/s00300-013-1440-3

Cite this article as:
Alcántara-Hernández, R.J., Centeno, C.M., Ponce-Mendoza, A. et al. Polar Biol (2014) 37: 403. doi:10.1007/s00300-013-1440-3

Abstract

Cyanobacterial microbial mats are highly structured communities commonly found in Antarctic inland waters including melt streams. These benthic microbial associations comprise a large number of microorganisms with different metabolic capacities, impacting nutrient dynamics where established. The denitrification process is a feasible nitrogen loss pathway and a biological source of nitrous oxide, a potent greenhouse gas that also promotes ozone depletion. Potential denitrifiers from five microbial mats were characterized using a PCR-DGGE (denaturing gradient gel electrophoresis) approach. Molecular markers encoding for key enzymes in the denitrification process (nirK, nirS and nosZ) were used. Fingerprints were obtained for the five sampled mats and compared for two successive years. Distance analysis showed that despite the sampled year, the denitrifying genetic potential was similar between most of the sites when represented in Euclidean space. The number of dominant denitrifiers detected for each sample ranged between 6 and 18 for nirK, 4–10 for nirS and 6–17 for nosZ. The seventy-two sequenced phylotypes showed 80–98 % identity to previously reported environmental sequences from water column, sediments and soil samples. These results suggest that Antarctic microbial mats have a large denitrification potential, previously uncharacterized and composed by both site-specific and common phylotypes belonging mainly to Alpha-, Beta- and Gammaproteobacteria.

Keywords

Cyanobacterial microbial mat Denitrifiers PCR-DGGE Maritime Antarctica 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Rocio J. Alcántara-Hernández
    • 1
  • Carla M. Centeno
    • 1
  • Alejandro Ponce-Mendoza
    • 2
  • Silvia Batista
    • 3
  • Martin Merino-Ibarra
    • 4
  • Julio Campo
    • 5
  • Luisa I. Falcón
    • 1
  1. 1.Laboratorio de Ecología Bacteriana y Epigenética, Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexicoMexico
  2. 2.Centro Nacional de Investigación Disciplinaria en Conservación y Mejoramiento de Ecosistemas Forestales, INIFAPMexicoMexico
  3. 3.Unidad de Microbiología MolecularInstituto de Investigaciones Biológicas Clemente Estable (MEC)MontevideoUruguay
  4. 4.Unidad Académica de Ecología Marina, Instituto de Ciencias del Mar y LimnologíaUniversidad Nacional Autónoma de MéxicoMexicoMexico
  5. 5.Laboratorio de Biogeoquímica Terrestre y Clima, Instituto de EcologíaUniversidad Nacional Autónoma de MéxicoMexicoMexico