Abstract
Kiwifruit was transformed with a soybean β-1,3-endoglucanase (EC 3.2.1.39) cDNA under the control of the cauliflower mosaic virus (CaMV) 35S RNA promoter. The introduced gene was expressed in young leaves of the transformants. Assays of protein extracts from young leaves showed an increase in enzyme activity in many transformants, the transformant with the highest level of enzyme activity having an about sixfold increase over the control plants. When leaves from control and three transformants were inoculated with Botrytis cinerea, which causes gray mold disease, the disease lesion areas for two transformants were smaller than on control plants.
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Received: 5 March 1998 / Revision received: 19 October 1998 / Accepted: 27 October 1998
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Nakamura, Y., Sawada, H., Kobayashi, S. et al. Expression of soybean b-1,3-endoglucanase cDNA and effect on disease tolerance in kiwifruit plants. Plant Cell Reports 18, 527–532 (1999). https://doi.org/10.1007/s002990050616
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DOI: https://doi.org/10.1007/s002990050616