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PDV2 has a dosage effect on chloroplast division in Arabidopsis

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PDV2 has a dosage effect on chloroplast division in Arabidopsis thaliana , but this effect may vary in different plants.

Abstract

Chloroplasts have to be divided as plants grow to maintain an optimized number in the cell. Chloroplasts are divided by protein complexes across the double membranes from the stroma side to the cytosolic side. PDV2 is a chloroplast division protein on the chloroplast outer membrane. It recruits the dynamin-related GTPase ARC5 to the division site. The C-terminus of PDV2 and the C-terminus of ARC6 interact in the intermembrane space, which is important for the localization of PDV2. Previously, it was shown that overexpression of PDV2 can increase the division of chloroplasts in Arabidopsis and moss, so the authors concluded that PDV2 determines the rate of chloroplast division in land plants. PDV2 was also shown to inhibit the GTPase activity of ARC5 by in vitro experiment. These results look to be contradictory. Here, we identified a null allele of PDV2 in Arabidopsis and studied plants with different levels of PDV2. Our results suggested that the chloroplast division phenotype in Arabidopsis is sensitive to the level of PDV2, while this is not the case for ARC6. The level of PDV2 protein is reduced sharply in fast-growing leaves, while the level of ARC6 is not. The levels of PDV2 and ARC6 in several other plant species at different developmental stages were also investigated. The results indicated that their expression pattern varies in different species. Thus, PDV2 is an important positive factor of chloroplast division with an apparent dosage effect in Arabidopsis, but this effect for different chloroplast division proteins in different plants may vary.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (31070162, 31501090, and 31400635).

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    Authors and Affiliations

    1. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China

      Ning Chang, Qingqing Sun, Yiqiong Li, Jinglei Hu, Xiaomin Liu & Hongbo Gao

    2. Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

      Yajuan Mu & Yue Feng

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    1. Ning Chang
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    2. Qingqing Sun
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    3. Yiqiong Li
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    7. Xiaomin Liu
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    Correspondence to Xiaomin Liu.

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    The authors claim no competing interests.

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    Communicated by Q. Zhao.

    N. Chang and Q. Sun contributed equally to this work.

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    Chang, N., Sun, Q., Li, Y. et al. PDV2 has a dosage effect on chloroplast division in Arabidopsis . Plant Cell Rep 36, 471–480 (2017). https://doi.org/10.1007/s00299-016-2096-6

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