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Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis

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Abstract

Key message

Our study is the first to demonstrate that PSK1 , a SKP1 -like gene homologue, is involved in salinity tolerance. Our functional characterization of PSK1 provides new insights into tree peony development.

Abstract

A homologous gene of S-phase kinase-associated protein1 (SKP1) was cloned from tree peony (Paeonia suffruticosa) and denoted as PSK1. The 462-bp open reading frame of PSK1 was predicted to encode a protein comprising 153 amino acids, with a molecular mass of 17 kDa. The full-length gene was 1,634 bp long and included a large 904-bp intron. PSK1 transcription was detected in all tissues, with the highest level observed in sepals, followed by leaves. Under salinity stress, overexpression of PSK1 in Arabidopsis resulted in increased germination percentages, cotyledon greening, and fresh weights relative to wild-type plants. Furthermore, transgenic Arabidopsis lines containing 35S::PSK1 displayed increased expression of genes that would be essential for reproduction and growth under salinity stress: ASK1, LEAFY, FT, and CO involved in flower development and flowering time as well as P5CS, RAB18, DREB, and SOD1-3 contributing to salinity tolerance. Our functional characterization of PSK1 adds to global knowledge of the multiple functions of previously explored SKP1-like genes in plants and sheds light on the molecular mechanism underlying its role in salinity tolerance. Our findings also provide information on the function and molecular mechanism of PSK1 in tree peony flower development, thereby revealing a theoretical basis for regulation of flowering and conferral of salinity tolerance in tree peony.

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Abbreviations

CaMV:

Cauliflower mosaic virus

DREB:

Dehydration-responsive element-binding protein

MDA:

Malondialdehyde

MS:

Murashige and Skoog

ORF:

Open reading frame

P5CS:

Δ1-Pyrroline-5-carboxylate synthase

qPCR:

Quantitative real-time PCR

RAB18:

Ras-related protein

RACE:

Rapid amplification of cDNA ends

ROS:

Reactive oxygen species

RT-PCR:

Reverse transcription-PCR

SOD:

Superoxide dismutase

WT:

Wild type

CO :

CONSTANS

FT :

Flowering locus T

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Acknowledgements

This study was financially supported by the National Natural Science Foundation of China (Grant Nos. 31272201 and 31471909).

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    Authors and Affiliations

    1. Key Laboratory of Plant Resources/Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

      Hongxu Ren, Jin Zhu, Liangsheng Wang, Zheng’an Liu & Qingyan Shu

    2. University of Chinese Academy of Sciences, Beijing, 100049, China

      Jin Zhu

    3. Landscape and Forestry College, Qingdao Agricultural University, Qingdao, 266109, China

      Qing Hao & Shouchen Huang

    4. International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, Beijing, 100102, China

      Zhimin Gao

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    1. Qing Hao
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    Communicated by B. Li.

    Qing Hao and Hongxu Ren contributed equally to this work.

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    299_2016_2066_MOESM3_ESM.jpg

    ESM_S3. Comparison of stress response of PSK1-overexpressing Arabidopsis transgenic plants (lines 7 - 9) and the WT. (A) Germination percentages of transgenic lines and WT plants calculated 1 days after keeping at 22 ºC in medium containing 0-125 mM NaCl. (B) Cotyledon greening rates of transgenic lines and WT plants growing in medium containing 0–125 mM NaCl for 1 week. (C) Root elongation measurement of 7-day-old plants maintained in medium for 1 week. Each column represents the average of three replicates, and the bar indicates SDs. The different characters above each column represent various significant differences at p < 0.05 (JPEG 3118 kb)

    299_2016_2066_MOESM4_ESM.jpg

    ESM_S4. Expression analysis of SOS1-3 in Arabidopsis plants (lines 7-9 and the WT) treated with 150 mM NaCl for 0–12 h. (A) SOS1 (B) SOS2 (C) SOS3. The different characters above each column represent various significant differences at p < 0.05 (JPEG 2450 kb)

    Supplementary material 5 (XLSX 12 kb)

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    Hao, Q., Ren, H., Zhu, J. et al. Overexpression of PSK1, a SKP1-like gene homologue, from Paeonia suffruticosa, confers salinity tolerance in Arabidopsis. Plant Cell Rep 36, 151–162 (2017). https://doi.org/10.1007/s00299-016-2066-z

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