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Transcriptomic analysis reveals key early events of narciclasine signaling in Arabidopsis root apex

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Histochemical staining and RNA-seq data demonstrated that the ROS- and plant hormone-regulated stress responses are the key early events of narciclasine signaling in Arabidopsis root cells.

Abstract

Narciclasine, an amaryllidaceae alkaloid isolated from Narcissus tazetta bulbs, employs a broad range of functions on plant development and growth. However, its molecular interactions that modulate these roles in plants are not fully understood. To elucidate the global responses of Arabidopsis roots to short-term narciclasine exposure, we first measured the accumulation of H2O2 and O2 – with histochemical staining, and then profiled the gene expression pattern in Arabidopsis root tips treated with 0.5 µM narciclasine across different exposure times by RNA-seq. Physiological measurements showed a significant increase in H2O2 began at 30–60 min of narciclasine treatment and O2 – accumulated by 120 min. Compared with controls, 236 genes were upregulated and 54 genes were downregulated with 2 h of narciclasine treatment, while 968 genes were upregulated and 835 genes were downregulated with 12 h of treatment. The Gene Ontology analysis revealed that the differentially expressed genes were highly enriched during oxidative stress, including those involved in the “regulation of transcription”, “response to oxidative stress”, “plant–pathogen interaction”, “ribonucleotide binding”, “plant cell wall organization”, and “ribosome biogenesis”. Moreover, Kyoto Encyclopedia of Genes and Genomes pathway enrichment statistics suggested that carbohydrate metabolism, amino acid metabolism, amino sugar and nucleotide sugar metabolism, and biosynthesis of phenylpropanoid and secondary metabolites were significantly inhibited by 12 h of narciclasine exposure. Hence, our results demonstrate that hormones and H2O2 are important regulators of narciclasine signaling and help to uncover the factors involved in the molecular interplay between narciclasine and phytohormones in Arabidopsis root cells.

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Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (31560345, 31260166 and 31360185), the earmarked fund for China Agriculture Research System (CARS-07-13.5) and Doctoral Scientific Fund Project of Shanxi Academy of Agricultural Sciences (YBSJJ1410). We thank Professor Wenyu Liang for valuable advice on ROS measurements and Xiaoran Li for critical reading of the manuscript.

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    1. School of Life Science, Ningxia University, Yinchuan, 750021, People’s Republic of China

      Xiaoning Cao, Fei Ma, Tingting Xu, Gaihong Li, Qian Su & XiaoFan Na

    2. Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Taiyuan, 030000, People’s Republic of China

      Xiaoning Cao, Junjie Wang, Sichen Liu & Zhijun Qiao

    3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan, People’s Republic of China

      Xiaoning Cao, Junjie Wang, Sichen Liu & Zhijun Qiao

    4. New Technology Application, Research and Development Center, Ningxia University, Yinchuan, 750021, People’s Republic of China

      Fei Ma

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    1. Xiaoning Cao
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    Communicated by K. Chong.

    X. Cao and F. Ma contributed equally to this work.

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    Cao, X., Ma, F., Xu, T. et al. Transcriptomic analysis reveals key early events of narciclasine signaling in Arabidopsis root apex. Plant Cell Rep 35, 2381–2401 (2016). https://doi.org/10.1007/s00299-016-2042-7

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