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A novel elicitor identified from Magnaporthe oryzae triggers defense responses in tobacco and rice

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Abstract

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Our studies indicate a potential important elicitor candidate which can aid in the fight against a worldwide disease, rice blast.

Abstract

In this study, we report the purification, identification, characterization, and gene cloning of a novel hypersensitive response-inducing protein elicitor (MoHrip2) secreted from an important pathogenic fungus, Magnaporthe oryzae. The protein fraction was isolated from the culture filtrate of M. oryzae and identified by de novo sequencing. The elicitor-encoding gene mohrip2 was cloned following sequence comparison and PCR amplification. This 459-bp gene encodes a 152-residue polypeptide that contains an 18-residue signal peptide and exhibits a pI of 4.72 and an apparent molecular mass of 16 kDa. The hypothetical protein, MoHrip2, was expressed in Escherichia coli, and both the recombinant and the endogenous protein caused necrotic lesions in tobacco leaves. In addition to phenolic compound deposition and alkalization of the extracellular medium, MoHrip2 also induced hydrogen peroxide production and nitric oxide accumulation in tobacco cells. Moreover, rice seedlings treated with MoHrip2 exhibited pronounced resistance to M. oryzae compared with control seedlings.

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Acknowledgments

We would like to thank Professor Guo-Liang Wang (Department of Plant Pathology, Ohio State University, Columbus) and Dr. Yuese Ning (IPP, Chinese Academy of Agricultural Sciences) for help of bioassay in rice seedlings. This work was supported by grants from the National Hi-Tech Research and Development Program of China (“863” Projects, Grant Nos. 2012AA101504 and 2011AA10A201).

Conflict of interest

The authors declare that they have no conflict of interest.

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  1. Mingjia Chen

    Present address: Department of Plant Biochemistry, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin-Luise-Str. 12-16, 14195, Berlin, Germany

Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Mingjia Chen, Caizhi Zhang, Qian Zi, Dewen Qiu, Wenxian Liu & Hongmei Zeng

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  1. Mingjia Chen
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  2. Caizhi Zhang
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Corresponding author

Correspondence to Hongmei Zeng.

Additional information

Communicated by Baochun Li.

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299_2014_1663_MOESM1_ESM.tif

Figure S1 Analysis of the MoHrip2 cDNA sequence using the SignalP 4.1 Server. MoHrip2 is predicted to contain an 18-residue signal peptide, which indicates that MoHrip2 is a secreted protein. (TIFF 1375 kb)

299_2014_1663_MOESM2_ESM.tif

Figure S2 Time course of the extracellular medium alkalinization in tobacco cell culture. The kinetics of the extracellular medium alkalinization induced by MoHrip2 (10 μmol l−1) in tobacco cell suspensions. A distinct pH increase in the elicitor-treated cell culture from 5.4 to 6.1 was observed for 30 min, and the pH subsequently slowly decreased to the initial pH value after 90 min. Each data point represents three replicates. The error bars represent ± SD of the mean. (TIFF 382 kb)

Table S1 Visual score rating for disease and plant responses (DOC 32 kb)

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Chen, M., Zhang, C., Zi, Q. et al. A novel elicitor identified from Magnaporthe oryzae triggers defense responses in tobacco and rice. Plant Cell Rep 33, 1865–1879 (2014). https://doi.org/10.1007/s00299-014-1663-y

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