Plant Cell Reports

, Volume 33, Issue 1, pp 143–151

Oryza sativa polyamine oxidase 1 back-converts tetraamines, spermine and thermospermine, to spermidine

  • Taibo Liu
  • Dong Wook Kim
  • Masaru Niitsu
  • Thomas Berberich
  • Tomonobu Kusano
Original Paper

DOI: 10.1007/s00299-013-1518-y

Cite this article as:
Liu, T., Kim, D.W., Niitsu, M. et al. Plant Cell Rep (2014) 33: 143. doi:10.1007/s00299-013-1518-y

Abstract

Key message

Oryza sativa polyamine oxidase 1 back-converts spermine (or thermospermine) to spermidine. Considering the previous work, major path of polyamine catabolism in rice plant is suggestive to be back-conversion but not terminal catabolism.

Abstract

Rice (Oryza sativa) contains seven genes encoding polyamine oxidases (PAOs), termed OsPAO1 to OsPAO7, based on their chromosomal number and gene ID number. We previously showed that three of these members, OsPAO3, OsPAO4 and OsPAO5, are abundantly expressed, that their products localize to peroxisomes and that they catalyze the polyamine back-conversion reaction. Here, we have focused on OsPAO1. The OsPAO1 gene product shares a high level of identity with those of Arabidopsis PAO5 and Brassica juncea PAO. Expression of OsPAO1 appears to be quite low under physiological conditions, but is markedly induced in rice roots by spermine (Spm) or T-Spm treatment. Consistent with the above finding, the recombinant OsPAO1 prefers T-Spm as a substrate at pH 6.0 and Spm at pH 8.5 and, in both cases, back-converts these tetraamines to spermidine, but not to putrescine. OsPAO1 localizes to the cytoplasm of onion epidermal cells. Differing in subcellular localization, four out of seven rice PAOs, OsPAO1, OsPAO3, OsPAO4 and OsPAO5, catalyze back-conversion reactions of PAs. Based on the results, we discuss the catabolic path(s) of PAs in rice plant.

Keywords

Back-conversion Oryza sativa OsPAO1 Polyamine Polyamine oxidase Thermospermine 

Supplementary material

299_2013_1518_MOESM1_ESM.docx (4.9 mb)
Supplementary material 1 (DOCX 5053 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Taibo Liu
    • 1
  • Dong Wook Kim
    • 1
  • Masaru Niitsu
    • 2
  • Thomas Berberich
    • 3
  • Tomonobu Kusano
    • 1
  1. 1.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  2. 2.Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan
  3. 3.Biodiversity and Climate Research Center, Laboratory CentreFrankfurt am MainGermany