Abstract
Wild soybean (Glycine soja L. G07256) exhibits a greater adaptability to soil bicarbonate stress than cultivated soybean, and recent discoveries show that TIFY family genes are involved in the response to several abiotic stresses. A genomic and transcriptomic analysis of all TIFY genes in G. soja, compared with G. max, will provide insight into the function of this gene family in plant bicarbonate stress response. This article identified and characterized 34 TIFY genes in G. soja. Sequence analyses indicated that most GsTIFY proteins had two conserved domains: TIFY and Jas. Phylogenetic analyses suggested that these GsTIFY genes could be classified into two groups. A clustering analysis of all GsTIFY transcript expression profiles from bicarbonate stress treated G. soja showed that there were five different transcript patterns in leaves and six different transcript patterns in roots when the GsTIFY family responds to bicarbonate stress. Moreover, the expression level changes of all TIFY genes in cultivated soybean, treated with bicarbonate stress, were also verified. The expression comparison analysis of TIFYs between wild and cultivated soybeans confirmed that, different from the cultivated soybean, GsTIFY (10a, 10b, 10c, 10d, 10e, 10f, 11a, and 11b) were dramatically up-regulated at the early stage of stress, while GsTIFY 1c and 2b were significantly up-regulated at the later period of stress. The frequently stress responsive and diverse expression profiles of the GsTIFY gene family suggests that this family may play important roles in plant environmental stress responses and adaptation.
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Acknowledgments
The authors thank Suk-Ha Lee (Seoul National University) for providing G. soja genome sequences. The authors thank Neil. Hobson (University of Alberta) for edit and suggestions to the manuscript. This study was supported by the National Natural Science Foundation of China (30570990, 30471059), the “863” project (2008AA10Z153), the National Major Project for Cultivation of Transgenic Crops (2008ZX08004), the Key Research Plan of Heilongjiang Province (GA06B103-3), the Innovation Research Group of NEAU (CXT004), and the Basic Research Preliminary Study Foundation of the Ministry of Science and Technology of the PRC (2003CCA03500).
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Communicated by H. Judelson.
Dan Zhu and Xi Bai contributed equally to this study.
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Zhu, D., Bai, X., Luo, X. et al. Identification of wild soybean (Glycine soja) TIFY family genes and their expression profiling analysis under bicarbonate stress. Plant Cell Rep 32, 263–272 (2013). https://doi.org/10.1007/s00299-012-1360-7
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DOI: https://doi.org/10.1007/s00299-012-1360-7