Plant Cell Reports

, Volume 31, Issue 11, pp 2057–2063

Dramatic genotypic difference in, and effect of genetic crossing on, tissue culture-induced mobility of retrotransposon Tos17 in rice

Authors

  • Chunjing Lin
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
    • Jilin Academy of Agricultural Sciences
  • Xiuyun Lin
    • Jilin Academy of Agricultural Sciences
  • Lanjuan Hu
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
  • Jingjing Yang
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
  • Tianqi Zhou
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
  • Likun Long
    • Jilin Academy of Agricultural Sciences
  • Chunming Xu
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
  • Shaochen Xing
    • Jilin Academy of Agricultural Sciences
  • Bao Qi
    • College of AgronomyJilin Agricultural University
    • Jilin Academy of Agricultural Sciences
    • Key Laboratory of Molecular Epigenetics of MOENortheast Normal University
Original Paper

DOI: 10.1007/s00299-012-1316-y

Cite this article as:
Lin, C., Lin, X., Hu, L. et al. Plant Cell Rep (2012) 31: 2057. doi:10.1007/s00299-012-1316-y

Abstract

Key message

We show for the first time that intraspecific crossing may impact mobility of the prominent endogenous retrotransposonTos17under tissue culture conditions in rice.

Abstract

Tos17, an endogenous copia retrotransposon of rice, is transpositionally active in tissue culture. To study whether there exists fundamental genotypic difference in the tissue culture-induced mobility of Tos17, and if so, whether the difference is under genetic and/or epigenetic control, we conducted this investigation. We show that dramatic difference in tissue culture-induced Tos17 mobility exists among different rice pure-line cultivars sharing the same maternal parent: of the three lines studied that harbor Tos17, two showed mobilization of Tos17, which accrued in proportion to subculture duration, while the third line showed total quiescence (immobility) of the element and the fourth line did not contain the element. In reciprocal F1 hybrids between Tos17-mobile and -immobile (or absence) parental lines, immobility was dominant over mobility. In reciprocal F1 hybrids between both Tos17-mobile parental lines, an additive or synergistic effect on mobility of the element was noticed. In both types of reciprocal F1 hybrids, clear difference in the extent of Tos17 mobility was noted between crossing directions. Given that all lines share the same maternal parent, this observation indicates the existence of epigenetic parent-of-origin effect. We conclude that the tissue culture-induced mobility of Tos17 in rice is under complex genetic and epigenetic control, which can be either enhanced or repressed by intraspecific genetic crossing.

Keywords

Tos17 mobilityTissue cultureGenetic crossingEpigenetic parent-of-origin effectOryza sativa L.

Copyright information

© Springer-Verlag 2012