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Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis

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Abstract

Laticifers are highly specialized cells present in over 20 plant families. They are well defined in planta. In vitro development of laticifers was also observed in some plants, but uncertain in the callus cultures of rubber tree, one of the most economically important latex producing plants. In the present study, we provide evidence that laticifer cells present in the callus cultures of rubber tree by histochemical and immunohistochemical studies. They present in the callus mainly as separate non-elongated form, a novel morphology different from the morphology of laticifer cells in planta, excluding their origin from explants. The occurring frequency of laticifer cells in the callus was genotype-dependent and negatively correlated with the somatic embryogenetic ability, suggesting that the presence of laticifer cells in the callus inhibit somatic embryogenesis in tissue culture of rubber tree. The genotypes PR107, RRIM600, Reyan8-79, and Reyan7-33-97 with lower embryogenetic ability compared to Haiken 2 had more laticifer cells, and laticifer clusters were only observed in these genotypes.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

KT:

6-Furfurylaminopurine

NAA:

1-Naphthaleneacetic acid

SD:

Standard deviation

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31070637) and National Nonprofit Institute Research Grant of CATAS-ITBB (No. ITBBZD0711).

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Authors and Affiliations

  1. MOA Key Laboratory for Tropical Crops Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China

    Deguan Tan, Xuepiao Sun & Jiaming Zhang

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  1. Deguan Tan
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  2. Xuepiao Sun
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  3. Jiaming Zhang
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Corresponding author

Correspondence to Jiaming Zhang.

Additional information

Communicated by F. Sato.

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299_2011_1019_MOESM1_ESM.doc

Autoinfluorescence of callus tissue of rubber tree. a1 and b1 Hand-made sections under normal light. a2 and b2 The same sections as in a1 and b1 under UV light showing the influorescence (DOC 128 kb)

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Tan, D., Sun, X. & Zhang, J. Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis . Plant Cell Rep 30, 1117–1124 (2011). https://doi.org/10.1007/s00299-011-1019-9

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  • DOI: https://doi.org/10.1007/s00299-011-1019-9

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