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Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death

  • Genetic Transformation and Hybridization
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An Erratum to this article was published on 13 June 2009

Abstract

The voltage-dependent anion channel (VDAC) is a major outer mitochondrial membrane protein. It is well documented that VDAC plays an important role in apoptosis, a kind of programmed cell death, in mammalian systems. However, little is known about the role of the plant counterpart during the process of plant-specific cell death such as pathogen-induced hypersensitive response. To address this issue, we isolated three VDAC full-length cDNAs (NtVDAC1–3) from Nicotiana tabacum. The deduced products, NtVDACs, share 78–85% identity and retain the conserved eukaryotic mitochondrial porin signature distal to their C-terminal regions. Mitochondrial localization of three NtVDACs in plant cells was confirmed via a green fluorescent protein fusion method. Then, we addressed the main issue concerning pathogenesis relation. The N. benthamiana orthologues of NtVDACs were upregulated by challenge with the non-host pathogen Pseudomonas cichorii, but not after challenge with the virulent pathogen P. syringae pv. tabaci. Both the pharmaceutical inhibition of VDAC and silencing of NbVDACs genes compromised the non-host resistance against P. cichorii, suggesting the involvement of VDACs in defense against non-host pathogen. Involvement of NbVDACs in Bax-mediated cell death was also verified using a similar approach.

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Abbreviations

ANT:

Adenine nucleotide translocator

CsA:

Cyclosporine A

DCFH-DA:

2′,7′-Dichlorodihydrofluorescein diacetate

DEX:

Dexamethasone

DIDS:

Dihydro-4,4′ diisothiocyanostilbene-2,2′-disulfonic acid

GFP:

Green fluorescent protein

HA:

Hemagglutinin

HR:

Hypersensitive response

MPS:

Mitochondrial porin signature

MPT:

Mitochondrial permeability transition

PCD:

Programmed cell death

PCR:

Polymerase chain reaction

PR:

Pathogenesis-related

PTP:

Permeability transition pore

ROS:

Reactive oxygen species

TRV:

Tobacco rattle virus

VDAC:

Voltage-dependent anion channel

VIGS:

Virus-induced gene silencing

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Acknowledgments

We acknowledge Drs I. Mitsuhara, Y. Niwa, Y. Ichinose, T. Shirakawa, H. Takahashi, D. Baulcombe, N.-H. Chua, and S. P. Dinesh-Kumar for providing vectors and bacterial strains. Dr. T. Berberich is acknowledged for critically reading the manuscript. This work was supported in part by Grant-in-Aid for Scientific Research for Plant Graduate Student from the Nara Institute of Science and Technology (supported by the Ministry of Education, Culture, Sports, Science, and Technology of Japan) to C. Tateda and Grant-in-Aids from the Japan Society for the Promotion of Science to T. Kusano (19658039) and Y. Takahashi (19780002), the Saito Gratitude Foundation to C. Tateda and the Sumitomo Foundation to Y. Takahashi.

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Authors and Affiliations

  1. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan

    Chika Tateda, Koji Yamashita, Fumio Takahashi, Tomonobu Kusano & Yoshihiro Takahashi

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  1. Chika Tateda
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  2. Koji Yamashita
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  3. Fumio Takahashi
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  4. Tomonobu Kusano
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  5. Yoshihiro Takahashi
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Corresponding author

Correspondence to Tomonobu Kusano.

Additional information

Communicated by R. Reski.

The nucleotide sequence reported in this paper has been submitted to DDBJ under the following accession numbers: NtVDAC1 (AB286176), NtVDAC2 (AB286177), and NtVDAC3 (AB286178).

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-009-0727-x

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Tateda, C., Yamashita, K., Takahashi, F. et al. Plant voltage-dependent anion channels are involved in host defense against Pseudomonas cichorii and in Bax-induced cell death. Plant Cell Rep 28, 41–51 (2009). https://doi.org/10.1007/s00299-008-0630-x

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