Plant Cell Reports

, Volume 26, Issue 7, pp 977–987

Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization

  • Andrew E. Newhouse
  • Franziska Schrodt
  • Haiying Liang
  • Charles A. Maynard
  • William A. Powell
Genetic Transformation and Hybridization

DOI: 10.1007/s00299-007-0313-z

Cite this article as:
Newhouse, A.E., Schrodt, F., Liang, H. et al. Plant Cell Rep (2007) 26: 977. doi:10.1007/s00299-007-0313-z

Abstract

The American elm (Ulmus americana L.) was once one of the most common urban trees in eastern North America until Dutch-elm disease (DED), caused by the fungus Ophiostoma novo-ulmi, eliminated most of the mature trees. To enhance DED resistance, Agrobacterium was used to transform American elm with a transgene encoding the synthetic antimicrobial peptide ESF39A, driven by a vascular promoter from American chestnut. Four unique, single-copy transgenic lines were produced and regenerated into whole plants. These lines showed less wilting and significantly less sapwood staining than non-transformed controls after O. novo-ulmi inoculation. Preliminary observations indicated that mycorrhizal colonization was not significantly different between transgenic and wild-type trees. Although the trees tested were too young to ensure stable resistance was achieved, these results indicate that transgenes encoding antimicrobial peptides reduce DED symptoms and therefore hold promise for enhancing pathogen resistance in American elm.

Keywords

Ulmus americanaOphiostoma novo-ulmiDutch elm disease resistanceTransgenicMycorrhizae

Abbreviations

DED

Dutch-elm disease

GUS

Beta-glucuronidase

SSC

Saline sodium citrate

SDS

Sodium dodecyl sulfate (also known as sodium lauryl sulfate)

PDB

Potato dextrose broth

PDA

Potato dextrose agar

MIC

Minimum inhibitory concentration

AMP

Anti-microbial peptide

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Andrew E. Newhouse
    • 1
  • Franziska Schrodt
    • 1
  • Haiying Liang
    • 2
  • Charles A. Maynard
    • 3
  • William A. Powell
    • 1
  1. 1.Faculty of Environmental and Forest BiologySUNY College of Environmental Science and ForestrySyracuseUSA
  2. 2.Department of Genetics and BiochemistryClemson UniversityClemsonUSA
  3. 3.Faculty of Forest and Natural Resources ManagementSUNY College of Environmental Science and ForestrySyracuseUSA