Plant Cell Reports

, Volume 24, Issue 12, pp 743–749

AFLP analysis of nephthytis (Syngonium podophyllum Schott) selected from somaclonal variants

  • J. Chen
  • R. J. Henny
  • P. S. Devanand
  • C. T. Chao
Genetics and Genomics

DOI: 10.1007/s00299-005-0032-2

Cite this article as:
Chen, J., Henny, R.J., Devanand, P.S. et al. Plant Cell Rep (2006) 24: 743. doi:10.1007/s00299-005-0032-2

Abstract

This study analyzed genetic differences of 19 cultivars selected from somaclonal variants of Syngonium podophyllum Schott along with their parents as well as seven additional Syngonium species and six other aroids using amplified fragment length polymorphism (AFLP) markers generated by 12 primer sets. Among the 19 somaclonal cultivars, ‘Pink Allusion’ was selected from ‘White Butterfly’. Tissue culture of ‘Pink Allusion’ through organogenesis resulted in the development of 13 additional cultivars. Self-pollination of ‘Pink Allusion’ obtained a cultivar, ‘Regina Red Allusion’, and tissue culture propagation of ‘Regina Red Allusion’ led to the release of five other cultivars. The 12 primer sets generated a total of 1,583 scorable fragments from all accessions, of which 1,284 were polymorphic (81.9%). The percentages of polymorphic fragments within ‘White Butterfly’ and ‘Regina Red Allusion’ groups, however, were only 1.2% and 0.4%, respectively. Jaccard's similarity coefficients among somaclonal cultivars derived from ‘White Butterfly’ and ‘Regina Red Allusion’, on average, were 0.98 and 0.99, respectively. Seven out of the 15 cultivars from the ‘White Butterfly’ group and three out of six from the ‘Regina Red Allusion’ group were clearly distinguished by AFLP analysis as unique fragments were associated with respective cultivars. The unsuccessful attempt to distinguish the remaining eight cultivars from the ‘White Butterfly’ group and three from the ‘Regina Red Allusion’ group was not attributed to experimental errors or the number of primer sets used; rather it is hypothesized to be caused by DNA methylation and/or some rare mutations. This study also calls for increased genetic diversity of cultivated Syngonium as they are largely derived from somaclonal variants.

Keywords

AFLP analysis Aroids Ornamental foliage plants Somaclonal variants Syngonium 

Abbreviations

AFLP: Amplified fragment length polymorphism PCOA: Principal coordinate analysis PCR: Polymerase chain reaction UPGMA: Unweighted pair group method with arithmetic averages 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • J. Chen
    • 1
  • R. J. Henny
    • 1
  • P. S. Devanand
    • 2
  • C. T. Chao
    • 2
  1. 1.Environmental Horticulture Department and Mid-Florida Research and Education CenterUniversity of Florida, IFASApopkaUSA
  2. 2.Department of Botany and Plant SciencesUniversity of California – RiversideRiversideUSA

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