Rheumatology International

, Volume 18, Issue 1, pp 5–10

Detection of anti-recombinant ββ2-glycoprotein 1 and anti-recombinant ββ2-glycoprotein 1 fifth-domain antibodies in sera from patients with systemic lupus erythematosus

  • Yang Cheng-De
  • Chen Shun-Le
  • Shen Nan
  • Qi Ming
  • Xue Feng
ORIGINAL ARTICLE

DOI: 10.1007/s002960050046

Cite this article as:
Cheng-De, Y., Shun-Le, C., Nan, S. et al. Rheumatology International (1998) 18: 5. doi:10.1007/s002960050046

Abstract

Two kinds of plasmid expression vectors which expressed β2-glycoprotein 1(β2GP1) and the fifth domain of β2-glycoprotein 1 (β2GP1-D5) were constructed respectively in this study. The antigenicity of recombinant β2GP1 (rβ2GP1) and β2GP1-D5 (rβ2GP1-D5) was identified by immunoblots using rabbit anti-β2GP1 antibodies, and the recombinant proteins were purified. Both anti-rβ2GP1 and anti-β2GP1-D5 antibodies in 112 patients were detected by ELISA using rβ2GP1 and rβ2GP1-D5 as coating antigens. A significant statistical correlation (r=0.667, P<0.01) between the levels of anti-β2GP1 and anticardiolipin (ACL) antibodies was found. The presence of anti-rβ2GP1 antibodies was associated with an increased frequency of history of thrombosis and/or recurrent abortion; hence anti-rβ2GP1 assay provided better specificity than conventional ACL assay. Detection of anti-rβ2GP1 antibodies may be of potential value in evaluating the risk of thrombosis and/or symptoms associated with other antiphospholipid syndromes (APS). The binding of anti-rβ2GP1 from the sera of patients with APS to rβ2GP1 was inhibited by rβ2GP1-D5. Meanwhile, of 28 patients who had positive anti-rβ2GP1 antibodies in sera, 27 (96.4%) had positive anti-rβ2GP1-D1 antibodies. This indicated that the antigenic epitope of β2GP1 may be located in its fifth domain.

Key wordsβ2-Glycoprotein 1Anticardiolipin antibodySystemic lupus erythematosus

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Yang Cheng-De
    • 1
  • Chen Shun-Le
    • 2
  • Shen Nan
    • 2
  • Qi Ming
    • 1
  • Xue Feng
    • 2
  1. 1.Department of Clinical Immunology, Jin-ling Hospital, School of Medicine, Nanjing University, 305 Zhong-Shan Dong Road, Nanjing, China 210002CN
  2. 2.Department of Clinical Immunology and Rheumatology, Ren-ji Hospital, Shanghai Second Medical University, 145 Shan-Dong Zhong Road, Shanghai, China 200001CN