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Serum progranulin irrelated with Breg cell levels, but elevated in RA patients, reflecting high disease activity

  • Original Article - Observational Research
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Abstract

Soluble progranulin (PGRN) is known to directly regulate regulatory T cells; however, whether PGRN levels are elevated in patients with rheumatoid arthritis and affect the regulatory subsets of B cells remain unknown. In this study, a total of 80 RA patients and 60 healthy controls were studied. Serum progranulin levels were determined using enzyme-linked immune-sorbent assay. A receiver operating characteristic (ROC) curve was used to evaluate the feasibility of serum PGRN as a biomarker for distinguishing patients with RA. CD19+CD5+GrB+ B cells were analyzed by flow cytometry in peripheral blood mononuclear cells (PBMCs). Serum progranulin levels in RA patients (median, 59.4 ng/mL) and in RA patients DAS28 > 5.1 (median, 71.98 ng/mL) were much higher than those in normal controls (median, 46.3 ng/mL; P < 0.001). The area under the ROC curve for progranulin levels was 0.705 for RA versus normal controls and the area under the ROC curve for progranulin levels in RA patients DAS28 > 5.1 was 0.977 versus normal controls (P < 0.001). Interestingly, serum progranulin and DAS28, CRP, ESR were all positively correlated in RA patients (P < 0.001). The number of CD19+CD5+GrB+ B cells was significantly higher in RA patients (P < 0.05); however, the level of Breg cells was not related to PGRN (P > 0.05). Our findings indicated that induction of PGRN expression may play a role in RA immune reaction and PGRN levels could be a useful biomarker in RA inflammatory response, but irrelated with Breg cell levels.

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Acknowledgments

Supported by Grant 15EZC2 from the Enze medical center scientific research funds and Grant 1401ky05 from Taizhou science and technology plan.

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Authors and Affiliations

  1. Department of Clinical Laboratory, Taizhou Hospital of Zhejiang Province, Affiliated Hospital of Wenzhou Medical University, Taizhou, Zhejiang Province, China

    Jiaxi Chen, Jun Li, Shiyong Chen, Chunlong Wu & Bo Shen

  2. Wenzhou Medical University, Wenzhou, Zhejiang Province, China

    Shuang Li, Jianfeng Shi & Lili Zhang

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  1. Jiaxi Chen
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  2. Shuang Li
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  3. Jianfeng Shi
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  4. Lili Zhang
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  5. Jun Li
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  7. Chunlong Wu
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  8. Bo Shen
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Correspondence to Bo Shen.

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Chen, J., Li, S., Shi, J. et al. Serum progranulin irrelated with Breg cell levels, but elevated in RA patients, reflecting high disease activity. Rheumatol Int 36, 359–364 (2016). https://doi.org/10.1007/s00296-015-3372-4

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  • DOI: https://doi.org/10.1007/s00296-015-3372-4

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