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TNF-α gene polymorphisms in Iranian Azeri Turkish patients with Behcet’s Disease

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Abstract

Genetic factors that predispose individuals to Behcet’s disease (BD) are considered to play an important role in the development of the disease. The serum level of tumor necrosis factor (TNF) is elevated in patients with BD, and a dramatic response to anti-TNF-α antibody treatment further supports the role of TNF in BD. We investigated the distribution of TNF-α promoter −1031T/C and −308G/A polymorphisms in 53 BD patients of Iranian Azeri Turks and 79 matched healthy controls, via the PCR–RFLP technique. The frequency of the TNF-α −1031C allele was significantly higher in Behcet’s patients than in healthy controls (p < 0.0001, OR = 3.08; 95% CI = 1.73–5.47), whereas the frequency of the TNF-α −308A allele was similar in the two compared groups. The frequency of CG haplotype was significantly higher (p < 0.0001, OR = 3.42; 95% CI = 1.89–6.18), and that of the TA haplotype was significantly lower in BD patients than in healthy controls. These results suggest that TNF-α is a susceptibility gene for BD in patients from Iranian Azeri Turk ethnic group.

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Abbreviations

BD:

Behcet’s disease

TNF-α:

Tumor necrosis factor-alpha

HLA:

Human leukocyte antigen

PCR–RFLP:

Polymerase chain reaction–restriction fragment length polymorphism

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Acknowledgments

The authors would like to thank all participating patients. This project was financially supported by Center of Excellence for Biodiversity (University of Tabriz).

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Correspondence to Mortaza Bonyadi.

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Bonyadi, M., Jahanafrooz, Z., Esmaeili, M. et al. TNF-α gene polymorphisms in Iranian Azeri Turkish patients with Behcet’s Disease. Rheumatol Int 30, 285–289 (2009). https://doi.org/10.1007/s00296-009-1134-x

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  • DOI: https://doi.org/10.1007/s00296-009-1134-x

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